Pregnancy rate and pregnancy loss after transfer of in vivo or in vitro derived equine embryos

McCue, Patrick M.; Ferris, Ryan A.; Stokes, J.E.; Hatzel, J.N.; Trundell, David A.; Carnevale, E.M.

doi:10.1016/j.jevs.2016.04.058

Cited by 6 publications

(4 citation statements)

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“…The breeding technique that resulted in the highest EEL was OPU-ICSI-ET. This is in agreement with other studies in horses [40,41] and cattle, suggesting that IVP embryos are of lower quality than in vivo derived embryos/pregnancies [42].…”

Section: Discussionsupporting

confidence: 93%

A retrospective comparison of the efficiency of different assisted reproductive techniques in the horse, emphasizing the impact of maternal age

2019

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Section: Discussionsupporting

confidence: 93%

A retrospective comparison of the efficiency of different assisted reproductive techniques in the horse, emphasizing the impact of maternal age

2019

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“…In this respect, McCue et al . reported that the likelihood of pregnancy is almost 20% lower for freshly transferred equine IVP embryos than for in vivo developed, flushed embryos . IVP embryos are probably less susceptible to cryopreservation‐induced damage because they are small and do not have a confluent embryonic capsule at the time of blastocyst formation .…”

Section: Discussionmentioning

confidence: 99%

“…<3 mm 3 days n = 1 (2) 3-5.8 mm 4 days n = 6 (12) 5.8-8.6 mm 5 days n = 16 (33) 8.6-13.4 mm 6 days n = 24 (49) >13.4 mm 7 days n = 2 (4) [19]. IVP embryos are probably less susceptible to cryopreservation-induced damage because they are small and do not have a confluent embryonic capsule at the time of blastocyst formation [2,11].…”

Section: Number Of Pregnancies (Frequency In %)mentioning

confidence: 99%

Factors affecting the likelihood of pregnancy and embryonic loss after transfer of cryopreserved in vitro produced equine embryos

Claes

Cuervo‐Arango

Broek

et al. 2018

Equine Veterinary Journal

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Summary Background In vitro embryo production (IVEP) is increasingly popular but data assessing the outcome of transferred embryos are scarce. Objectives To determine the likelihood of pregnancy and embryonic loss after transfer of frozen‐thawed IVP embryos and identify factors influencing success. Study design Retrospective clinical study. Methods Blastocysts (n = 261) were produced from immature oocytes of Warmblood mares (n = 116) by Intracytoplasmic Sperm Injection (ICSI) and in vitro culture, and cryopreserved. Thawed IVP embryos were transferred into recipient mares on day 4, 5 or 6 after ovulation. The influence of donor mare (age, reproductive history), recipient mare (age, reproductive status, management; in‐house vs. outpatient, day post‐ovulation), embryo (interval from ICSI to blastocyst formation) and management factors (season when ovum pickup was performed, year and method of transfer) on likelihood of pregnancy and embryonic loss was examined, and the developmental stage of the IVP embryo at the time of transfer was estimated. Results The percentage of mares pregnant 7–10, 23 and 37 days after transfer was 56% (147/261), 49% (129/261), and 48% (124/261), respectively. Development of IVP embryos after transfer equated to day 5 or 6 in vivo embryos. With the exception of year of transfer, none of the factors had an impact on the likelihood of pregnancy or embryonic loss. Nevertheless, the likelihood of pregnancy tended to be lower for IVP embryos from infertile mares or when embryos were transferred into recipient mares on day 6 after ovulation rather than on day 4 or 5. Finally, the diameter of the embryonic vesicle 7 days post transfer was lower for pregnancies that were lost compared to those that were maintained. Main limitations Small sample size in some of the donor and recipient mare categories. Conclusions Cryopreserved IVP embryos should be transferred into recipient mares on day 4 or 5 after ovulation and a slower rate of post transfer vesicle expansion indicates a higher risk of subsequent embryonic loss The Summary is available in Portuguese ‐ see Supporting Information

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“…The likelihood of pregnancy and foaling after transfer of frozen-thawed IVP embryos was 62% and 49% respectively, which is roughly comparable to fresh IVP embryos (McCue et al 2016) but still lower than reported for an ET program using freshly transferred in vivo-derived horse embryos (Cuervo-Arango et al 2018a). The two most important factors affecting foaling outcome were the speed of in vitro embryo development and the day of ovulation of the recipient mare at the time of ET.…”

Section: Discussionmentioning

confidence: 66%

Speed of in vitro embryo development affects the likelihood of foaling and the foal sex ratio

Claes

Cuervo‐Arango

Colleoni

et al. 2020

Reprod. Fertil. Dev.

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The success of invitro embryo production (IVEP) in horses has increased considerably during recent years, but little is known about the effect of the speed of invitro embryo development. Blastocysts (n=390) were produced by intracytoplasmic sperm injection of IVM oocytes from warmblood mares, cryopreserved, thawed and transferred into recipient mares on Days 3, 4, 5 or 6 after ovulation. The time required for invitro-produced (IVP) embryos to reach the blastocyst stage was recorded (Day 7 vs Day 8). The likelihood of foaling was affected by the speed of invitro embryo development and recipient day after ovulation at transfer. The odds ratio for foaling was ~0.63 for transfer of Day 8 (46%) compared with Day 7 (56%) IVP blastocysts. The highest likelihood of pregnancy (72%) and foaling (60%) was observed when IVP blastocysts were transferred to recipient mares on Day 4 after ovulation. Finally, the sex (colt:filly) ratio was higher after transfer of Day 7 (71%:29%) than Day 8 (54%:46%) IVP blastocysts, suggesting that the speed of embryo development is sex dependent. In conclusion, the speed of invitro embryo development in our IVEP system affects the likelihood of foaling and the sex of the foal.

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Pregnancy rate and pregnancy loss after transfer of in vivo or in vitro derived equine embryos

Cited by 6 publications

References 0 publications

A retrospective comparison of the efficiency of different assisted reproductive techniques in the horse, emphasizing the impact of maternal age

A retrospective comparison of the efficiency of different assisted reproductive techniques in the horse, emphasizing the impact of maternal age

Factors affecting the likelihood of pregnancy and embryonic loss after transfer of cryopreserved in vitro produced equine embryos

Speed of in vitro embryo development affects the likelihood of foaling and the foal sex ratio

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