Preimplantation genetic diagnosis (PGD): The Gothenburg experience

Hanson, Charles; Jakobsson, Ann-Helene; Sjögren, Anita; Lundin, Kersti; Nilsson, Johan; Wahlström, Jan; Hardarson, Thorir; Stevic, Justina; Darnfors, Catarina; Janson, Per Olof; Wikland, Matts; Hamberger, Lars

doi:10.1080/j.1600-0412.2001.080004331.x

Cited by 10 publications

(12 citation statements)

References 21 publications

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“…The cell samples were analysed by fluorescent in situ hybridization (FISH) of the sex chromosomes. This technique has been described in detail elsewhere (Hanson et al 2001b) and is briefly outlined below.…”

Section: Methodsmentioning

confidence: 99%

Longterm survival of transplanted human corneal epithelial cells and corneal stem cells

Egarth

Hellkvist

Claesson

et al. 2005

Acta Ophthalmologica Scandinavica

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Harvesting cells from removed sutures in combination with FISH enables the clinical study of cell survival in corneal transplants without jeopardizing functioning grafts. From the limited sample investigated, the following tentative conclusions can be made. Donor-derived epithelial cells can remain in conventional PKP for over 1 year. In combined stem cell and corneal grafts (HPCLK), donor-derived epithelial cells may also be retrieved at 1 year or beyond following surgery but the correlation between their presence and a remaining clear graft is uncertain.

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Section: Methodsmentioning

confidence: 99%

Longterm survival of transplanted human corneal epithelial cells and corneal stem cells

Egarth

Hellkvist

Claesson

et al. 2005

Acta Ophthalmologica Scandinavica

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“…The FISH procedure was performed mainly as described previously (H anson et al 2001a; H anson et al 2001b).…”

Section: Methodsmentioning

confidence: 99%

Fluorescence in situ hybridisation analysis and ovarian histology of women with Turner syndrome presenting with Y-chromosomal material: a correlation between oral epithelial cells, lymphocytes and ovarian tissue

et al. 2002

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The early detection of Y-chromosomal material in women with Turner syndrome (TS) is of great importance due to a relatively high risk of gonadal tumour development. Using fluorescence in situ hybridisation (FISH) analysis, we studied the presence of three different Y-specific sequences (SRY, Ycen and Yq12) in three different tissues (oral epithelial cells, lymphocytes and ovarian tissue) of twelve TS women. We have also described their ovarian histology. Two of the women (17%) had gonadal tumours. In five women where ovarian tissue was available, the presence of Y-chromosomal material in oral epithelial cells and lymphocytes correlated to the presence of Y-chromosomal material in the gonads. We therefore conclude that FISH analysis of oral epithelial cells and/or lymphocytes is a valuable complement to karyotyping for the early detection of Y-chromosomal material in TS women.

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“…Compared to conventional prenatal diagnosis, chorion villi sampling (CVS) and amniocentesis, PGD enables couples to avoid the termination of affected pregnancies, because only healthy embryos will be transferred. PGD may be used in order to study chromosome disorders and single‐gene defects, when the mutation in the family is known, as well as for sexing in X‐linked diseases (54). In order to perform PGD, one to two cells (blastomeres) are biopsied usually from a six‐ to eight‐cell embryo 3 days after oocyte retrieval (Fig.…”

Section: Art In Families With An Inherited Diseasementioning

confidence: 99%

Genetics and assisted reproduction technology

Aittomäki

Bergh

Hazekamp³

et al. 2005

Acta Obstet Gynecol Scand

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In the past 20 years, a significant improvement has been shown in the treatment for infertility in both women and men through the development of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Only donated sperm could be previously used for treatment; now oocytes can also be donated. Furthermore, the combination of IVF and ICSI with advanced genetic methods has made preimplantation genetic diagnosis possible for many genetic conditions. These methods enable genetic testing of the early human embryo by using only a single cell, one blastomere biopsied from the embryo, as the sample from which the diagnosis of many chromosome rearrangements and other inherited diseases can be made. It has also been established that a considerable proportion of infertility is caused by genetic defects, which have several implications for infertility treatment. The purpose of this review is to give a concise introduction on how genetics is involved in assisted reproduction technology to specialists who may not be working in this particular field of gynecology, but who would need some knowledge of this for proper care of their patients.

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Preimplantation genetic diagnosis (PGD): The Gothenburg experience

Cited by 10 publications

References 21 publications

Longterm survival of transplanted human corneal epithelial cells and corneal stem cells

Longterm survival of transplanted human corneal epithelial cells and corneal stem cells

Fluorescence in situ hybridisation analysis and ovarian histology of women with Turner syndrome presenting with Y-chromosomal material: a correlation between oral epithelial cells, lymphocytes and ovarian tissue

Genetics and assisted reproduction technology

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