Preliminary characterization of genetic strains in clariid species, Clarias gariepinus and Heterobranchus bidorsalis using microsatellite markers

Agbebi, O. T.; Ilaboya, D. E.; Adebambo, A.O.

doi:10.5897/ajb12.2465

“…Analysis across populations revealed a higher mean number of alleles in the cultured population (3.000 ± 0.724) than the wild (2.714 ± 0.286) with effective number of alleles at 1.705 ± 0.205 and 1.733 ± 0.230 respectively ( Table 5). The mean heterozygosity of 40.3% observed in this study is similar to the 44.3% observed by Agbebi et al [22] in a study comparing C. gariepinus and Heterobranchus bidorsalis, while the expected heterozygosity (0.361 ± 0.053) is lower than the 0.896 ± 0.111 reported by the same author. The lower expected heterozygosity may suggest reduced evolutionary potentials in these populations.…”

Section: Microsatellite Dnasupporting

confidence: 87%

“…The lower expected heterozygosity may suggest reduced evolutionary potentials in these populations. The Shannon's Information index (I=0.629 ± 0.143 and 0.591 ± 0.115) observed in this study, which are lower than previously reported Figures from similar studies [22,28] may suggest a lower or loss of genetic diversity in the studied populations.…”

Section: Microsatellite Dnacontrasting

confidence: 84%

“…Popoola et al [16,[21][22][23] reported percentage polymorphic loci of 68.5, 100, 89.9 and 74.7 for cultured), and ~93% (and 100% for cultured) respectively, which are higher than the 47.9% and 60.8% observed in this study for the wild and cultured populations respectively. In this present study, the cultured population showed a higher level of inherent genetic diversity and allele richness than the wild as revealed by indicators such as the percentage of polymorphic loci (%P), Number of Alleles (Na), Number of effective alleles (Ne), Shannon Information index (I), and expected heterozygosity (Nei's gene diversity).…”

Section: Resultscontrasting

confidence: 65%

See 1 more Smart Citation

Genetic Diversity in Cultured and Wild Population of Clarias gariepinus (Burchell, 1822) in Nigeria Using Random Amplified Polymorphic DNA (RAPD) and Microsatellite DNA

Awodiran¹,

Afolabi²

2018

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The population structure and genetic diversities of Clarias gariepinus from the cultured population at Chi Farm (Ajanla) and wild population at Asejire Reservoir (Asejire) were analysed using Random Amplified Polymorphic DNA (RAPD) and Microsatellite DNA markers. Using a CTAB protocol, genomic DNA was extracted from the caudal fins of 20 samples of live specimen collected from each population. Seven RAPD primers and seven pairs of microsatellite DNA primers were used to amplify different loci on the extracted genomic DNA by Polymerase Chain Reaction and the resultant DNA fragments were analysed on agarose gel. The RAPD primers amplified a total of 474 loci with 697 bands in all samples for the seven primers studied. The cultured population from Chi farm showed a total of 366 bands, while the wild population from Asejire Reservoir displayed 331 bands. The cultured population showed a negative inbreeding coefficient (F) of-0.173 ± 0.209, which statistically suggests excess heterozygosity, while a positive but low inbreeding coefficient of 0.042 ± 0.243 was estimated for the wild population. The Analysis of Molecular Variance (AMOVA) for both genetic markers indicated significant difference (p=0.01) between the two populations. The result of the study suggests a loss or ongoing loss of genetic variability, which needs conservation intervention in the two populations studied.

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“…Global ranking has placed Nigeria as the highest producer of catfish with total production of 1.2 million tonnes (Malomo, 2023) [11] . Catfish species have become a model fish for aquaculture due to its commercial value and favourable characteristic such as ability to withstand hash weather condition, omnivorous feeding, high reproduction, resistance to diseases and high growth rate (Agbebe et al, 2013) [1] . The two species of catfish that was investigated on are Clarias gariepinus and Heterobranchus bidorsalis and the genus Clarias can easily be differentiated from genus Heterobranchus by the presence of adipose fin in Heterobranchus and no adipose fin in Clarias (Moses & Olufeagba 2005) [12] .…”

Section: Introductionmentioning

confidence: 99%

Evolutionary relationship among two catfish species by Single Nucleotide Polymorphisms (SNPs) DNA markers

NO,

SM,

AT

et al. 2023

Int. J. Fish. Aquat. Stud.

0

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The three dams in Niger State, Nigeria, that were investigated for evolutionary relationship among two catfish species (Clarias gariepinus and Heterobrachus bidosalis) are Agaie/Lapai dam, Shiroro dam and Tagwai dam. Ten samples of each species were collected from the study dams and they were subjected to single nucleotide polymorphic genotyping. Four SNPs primers, which consist of growth hormone and early growth response gene were utilized to amplified the DNA. Bands were generated from all the samples from the study dams in all the primers used for the amplification, which gives strong indications that, all the samples been genotyped are rich in both growth hormone and early growth response gene. Further-more, two samples from each species from the study dams were sequenced, then subjected to Bio-edit software to draw the evolutionary relationship tree. The samples were grouped into two main branches by the dendrogram in Bio edit software that draws the evolutionary relationship tree, the first branch are made up of all the C. gariepinus samples and the second branch comprises of all the H. bidosalis samples from the three dams, the two main groups show by the dendrogram reveals that both species are evolutionarily not very close to each other and this also give the reassurance of the two species belonging to different species and genera.

show abstract

“…Global ranking has placed Nigeria as the highest producer of catfish with total production of 1.2 million tonnes (Malomo, 2023) [11] . Catfish species have become a model fish for aquaculture due to its commercial value and favourable characteristic such as ability to withstand hash weather condition, omnivorous feeding, high reproduction, resistance to diseases and high growth rate (Agbebe et al, 2013) [1] . The two species of catfish that was investigated on are Clarias gariepinus and Heterobranchus bidorsalis and the genus Clarias can easily be differentiated from genus Heterobranchus by the presence of adipose fin in Heterobranchus and no adipose fin in Clarias (Moses & Olufeagba 2005) [12] .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2023

Int. J. Fish. Aquat. Stud.

0

View full text Add to dashboard Cite

The three dams in Niger State, Nigeria, that were investigated for evolutionary relationship among two catfish species (Clarias gariepinus and Heterobrachus bidosalis) are Agaie/Lapai dam, Shiroro dam and Tagwai dam. Ten samples of each species were collected from the study dams and they were subjected to single nucleotide polymorphic genotyping. Four SNPs primers, which consist of growth hormone and early growth response gene were utilized to amplified the DNA. Bands were generated from all the samples from the study dams in all the primers used for the amplification, which gives strong indications that, all the samples been genotyped are rich in both growth hormone and early growth response gene. Further-more, two samples from each species from the study dams were sequenced, then subjected to Bio-edit software to draw the evolutionary relationship tree. The samples were grouped into two main branches by the dendrogram in Bio edit software that draws the evolutionary relationship tree, the first branch are made up of all the C. gariepinus samples and the second branch comprises of all the H. bidosalis samples from the three dams, the two main groups show by the dendrogram reveals that both species are evolutionarily not very close to each other and this also give the reassurance of the two species belonging to different species and genera.

show abstract

Preliminary characterization of genetic strains in clariid species, Clarias gariepinus and Heterobranchus bidorsalis using microsatellite markers

Cited by 9 publications

References 37 publications

Genetic Diversity in Cultured and Wild Population of Clarias gariepinus (Burchell, 1822) in Nigeria Using Random Amplified Polymorphic DNA (RAPD) and Microsatellite DNA

Genetic Diversity in Cultured and Wild Population of Clarias gariepinus (Burchell, 1822) in Nigeria Using Random Amplified Polymorphic DNA (RAPD) and Microsatellite DNA

Evolutionary relationship among two catfish species by Single Nucleotide Polymorphisms (SNPs) DNA markers

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