Preliminary crystallographic studies of a protein from Pachyrrhizus erosus

Abstract

Crystals of a protein extracted from the seeds of Pachyrrhizus erosus have been obtained by vapor-phase diffusion. The crystal belongs to the space group P4~2j2 or P432~2 with cell parameters a ----b ----62.52, c ----147.42 A. There is one protein molecule of 33 kDa in an asymmetric unit. A data set at 3.1 A has been collected on an area detector.

Macromolecular crystal twinning, lattice disorders and multiple crystals1

“…Lin et al (47) used dioxane in their study of a protein from Pachyrrhizus erosus: the droplet consisted of an equal volume of protein solution and the solution from the well. The crystals grew as octagonal plates with twinning in the direction of the c-axis within 10 days.…”