Termiticidal activity of wood vinegar from Toona sinensis (TS) sawdust has been evaluated against Coptotermes curvignathus. TS wood vinegar was produced at 250-300°C pyrolysis temperature, and a no-choice test was applied for evaluating termiticidal activity with thirty-three active termites. The concentrations of TS wood vinegar in the dried filter paper was prepared to 0% (solvent only), 2%, 4%, 6%, and 8%. The TS wood vinegar showed strong termiticidal activity and that concentrations of wood vinegar significantly influenced mortality of termite. The termite mortality increased when the concentration of wood vinegar increased, at a concentration of 8% all termites dead after 3 days tested. Forty constituents were characteristic on the basis of GC-MS data, and 33 components were identified. The organic compounds in TS wood vinegar were classified into carboxylic acids, phenols, ketones, amides, aldehydes, furans, esters, alcohols, sugar derivative, and ether, and among of this components, acetic acid comprised 57.43%. The contents of organic fraction of TS wood vinegar and largely acetic acid might be responsible for the termiticidal activity. Base on physicochemical properties, TS wood vinegar has a good quality as well.