Preliminary Evaluation of Noninvasive Microscopic Imaging Techniques for the Study of Vocal Fold Development

Boudoux, Caroline; Leuin, Shelby; Oh, Wang‐Yuhl; Suter, Melissa J.; Desjardins, Adrien E.; Vakoc, Benjamin J.; Bouma, Brett E.; Hartnick, Christopher J.; Tearney, G. J.

doi:10.1016/j.jvoice.2007.10.003

Cited by 16 publications

(10 citation statements)

References 44 publications

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“…Such a strong contrast may be better explained by the large difference between the epithelium, which exhibits a relatively low scattering, and the matrix-rich tissue beneath it, an effect also observed on porcine models. 32 Below the epithelium is a dark layer about 150 µm thick consistent with the fibrosis artifact observed on histologic images and possibly resulting from prolonged intubation. The dashed arrow points at a signal-poor layer.…”

Section: Spectrally Encoded Confocal Microscopymentioning

confidence: 77%

“…Our group 32 recently performed a comparative study of 4 optical microscopy techniques that were shown either to fit the dimensional criteria or to have the potential to be miniaturized: optical frequency domain imaging (OFDI), angle-resolved OFDI (AR-OFDI), full-field optical coherence microscopy (FF-OCM), and spectrally encoded confocal microscopy (SECM). Preliminary imaging of the porcine vocal fold allowed visualization of architectural, cellular, and subcellular features of the vocal apparatus at depths up to 2 mm (OFDI and AR-OFDI) and 400 µm (FF-OCM and SECM).…”

Section: Discussionmentioning

confidence: 99%

“…Preliminary imaging of the porcine vocal fold allowed visualization of architectural, cellular, and subcellular features of the vocal apparatus at depths up to 2 mm (OFDI and AR-OFDI) and 400 µm (FF-OCM and SECM). 32 While the preliminary results showed great promise, 2 questions remained. First, can these technologies be used to delineate the different layers of the lamina propria?…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Optical Microscopy of the Pediatric Vocal Fold

Boudoux¹,

Leuin²,

Oh³

et al. 2009

Arch Otolaryngol Head Neck Surg

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show abstract

Section: Spectrally Encoded Confocal Microscopymentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Optical Microscopy of the Pediatric Vocal Fold

Boudoux¹,

Leuin²,

Oh³

et al. 2009

Arch Otolaryngol Head Neck Surg

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show abstract

“…7 Preliminary imaging results with spectrally encoded confocal microscopy in porcine tissue demonstrate the potential for endomicroscopic imaging of the vocal folds. Images portraying epithelial cells, the basement membrane, and the lamina propria were obtained down to 375 μm deep in the tissue.…”

Section: Medical Applications Of Confocal Endomicroscopymentioning

confidence: 91%

Confocal Endomicroscopy: Instrumentation and Medical Applications

et al. 2011

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Advances in fiber optic technology and miniaturized optics and mechanics have propelled confocal endomicroscopy into the clinical realm. This high resolution, non-invasive imaging technology provides the ability to microscopically evaluate cellular and sub-cellular features in tissue in vivo by optical sectioning. Because many cancers originate in epithelial tissues accessible by endoscopes, confocal endomicroscopy has been explored to detect regions of possible neoplasia at an earlier stage by imaging morphological features in vivo that are significant in histopathologic evaluation. This technique allows real-time assessment of tissue which may improve diagnostic yield by guiding biopsy. Research and development continues to reduce the overall size of the imaging probe, increase the image acquisition speed, and improve resolution and field of view of confocal endomicroscopes. Technical advances will continue to enable application to less accessible organs and more complex systems in the body. Lateral and axial resolutions down to 0.5 μm and 3 μm, respectively, field of view as large as 800×450 μm, and objective lens and total probe outer diameters down to 350 μm and 1.25 mm, respectively, have been achieved. We provide a review of the historical developments of confocal imaging in vivo, the evolution of endomicroscope instrumentation, and the medical applications of confocal endomicroscopy.

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“…Previous experimental demonstrations of spectrally encoded imaging probes have utilized either 10 mm diameter grating within a prism-GRISM configuration [26] or approximately 7 mm x 10 mm rectangular gratings [27–29]. A sub-millimeter diameter SEE probe has been also demonstrated [18,22] utilizing a 350 μm diameter transmission grating which was fabricated by imprinting a linear grating pattern on an epoxy resin (Holographix LLC) at the angled tip of a large core fiber.…”

Section: Methods and Experimental Setupmentioning

confidence: 99%

Dual-channel spectrally encoded endoscopic probe

Engel

Genish

Rosenbluh

et al. 2012

Biomed. Opt. Express

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High quality imaging through sub-millimeter endoscopic probes provides clinicians with valuable diagnostics capabilities in hard to reach locations within the body. Spectrally encoded endoscopy (SEE) has been shown promising for such task; however, challenging probe fabrication and high speckle noise had prevented its testing in in vivo studies. Here we demonstrate a novel miniature SEE probe which incorporates some of the recent progress in spectrally encoded technology into a compact and robust endoscopic system. A high-quality miniature diffraction grating was fabricated using automated femtosecond laser cutting from a large bulk grating. Using one spectrally encoded channel for imaging and a separate channel for incoherent illumination, the new system has large depth of field, negligible back reflections and well controlled speckle noise which depends on the core diameter of the illumination fiber. Moreover, by using a larger imaging channel, higher groove density grating, shorter wavelength and broader spectrum, the new endoscopic system now allow significant improvements in almost all imaging parameter compared to previous systems, through an ultra-miniature endoscopic probe.

show abstract

Preliminary Evaluation of Noninvasive Microscopic Imaging Techniques for the Study of Vocal Fold Development

Cited by 16 publications

References 44 publications

Optical Microscopy of the Pediatric Vocal Fold

Optical Microscopy of the Pediatric Vocal Fold

Confocal Endomicroscopy: Instrumentation and Medical Applications

Dual-channel spectrally encoded endoscopic probe

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