Preliminary Phytochemical analysis and In vitro Antioxidant, FTIR Spectroscopy, Anti-diabetic activity of Acacia catechu ethanolic seed extract

Abstract

Objective: To evaluate the preliminary phytochemical analysis and in vitro antioxidant activity, anti-diabetic effect of ethanolic seed extract of Acacia catechu against the alpha amylase and alpha glucosidase digestive enzymes in the pancreas and small intestine. Methods: Preliminary phytochemical analysis was done by adopting the method of Evans. Antioxidant assay is performed by DPPH, ABTS and FRAP assay, Anti diabetic activity was determined by modified method of miller, the extract at different concentrations was tested for mammalian alpha amylase and alpha glucosidase enzyme inhibitory assay under the controlled experimental conditions and subjected to determination of absorbance. Results: The present study reveals the presence of few secondary metabolites and the extract exhibits potent Antioxidant activity and a concentration dependent inhibition of Alpha amylase and Alpha glucosidase. Conclusion: From the present study it can be concluded that ethanolic seed extract of Acacia catechu possessed marked in vitro antioxidant and anti-diabetic effect. The effect was plausibly due to the presence of phenolic contents of Acacia catechu.

LC-HRMS Profiling and Antidiabetic, Antioxidant, and Antibacterial Activities of Acacia catechu (L.f.) Willd

“…Phytochemicals such as phenolic and flavonoid isolated from various parts of the plants are used as antioxidant, antimicrobial, antiulcer, antidiabetic, anticancer, antihyperlipidemic, antidiabetic, and hepatoprotective candidates [79,80]. Previously, the IC 50 value for inhibition of α-amylase activity by A. catechu ethanol extract was reported as 341:20 ± 15:30 μg/mL by Lakshmi et al [2], while ethanol extract showed an inhibition of 67:8 ± 1:8 μg/mL in this study. Likewise, the methanolic extract of the A. catechu bark showed an IC 50 of 115 ± 4:0 mg/mL and 23:7 ± 0:7 mg/mL against α-glucosidase and α-amylase, respectively [34], whereas leaf extracts showed an IC 50 of 0.4977 mg/mL against α-glucosidase [81].…”

“…Acacia catechu (L.f.) Willd is a deciduous and gregarious tree with a light feathery crown native to Nepal, India, and Myanmar and one of the most promising medicinal plants of the family Fabaceae [1]. A. catechu has received attention as a potential source of bioactive secondary metabolites to be used for the formulation of pharmaceutical products [2,3]. Various parts of plant extracts were known to have strong antioxidant [2], antimicrobial [4], anti-inflammatory [5], antihyperglycemic [6], and immunomodulatory [7] activities.…”

“…A. catechu has received attention as a potential source of bioactive secondary metabolites to be used for the formulation of pharmaceutical products [2,3]. Various parts of plant extracts were known to have strong antioxidant [2], antimicrobial [4], anti-inflammatory [5], antihyperglycemic [6], and immunomodulatory [7] activities. Although several secondary metabolites have been identified from A. catechu, the molecules, catechin, epicatechin, and quercetin, are the principal contributor to therapeutical properties [8].…”

Study of the Effect of Lampeni (Ardisia humilis Vahl.) Planting Condition toward the Alpha-glucosidase Inhibition Activity in vitro

“…Inhibition of these hydrolytic enzymes is believed to be an attempt to control postprandial blood sugar levels. 2 Conventional DM management using chemical drugs such as biguanides, sulfonylureas, meglitinide, thiazolidinedione (TZD), dipeptidyl peptidase 4 (DPP-4) inhibitors, sodium-glucose cotransporter (SGLT2) inhibitors, and α-glucosidase inhibitors are known to cause some side effects. Some evidence of the side effects of synthetic hypoglycemic drugs such as hypoglycemia, digestive, and central nervous system disorders had been reported.…”

Antibacterial Activity of Acacia Catechu Seed Against Urinary Tract Pathogens

“…Pseudomonas predominantly causes urinary tract contamination, wound or burn infection, chronic otitis media, septicemia and so forth in people 6 . Work has been done which go for knowing the diverse antimicrobial and phyto-chemical constituents of medicinal plants and utilizing them for the treatment of microbial contaminations as conceivable adjust locals to chemically manufactured medications to which numerous irresistible microorganisms have turned out to be resistant 7 Acacia catechu is a moderate size deciduous, thorny tree common to Southern Asia and widely distributed in India.It is commonly known as "khair" and its various parts have been used since ancient times in Ayurvedic medicine 8,24,26 .In Ayurveda, is used in the treatment of cough, dysentery, throat infections, chronic ulcers and wounds 9,25 . Catechu is also reported for its anti-inflammatory,antimicrobial immunomodulatory, antipyretic, antidiarrhoeal and hypoglycemic [10][11][12][13] properties.…”