Preliminary responses of 2, 4-D and BAP on callus initiation of an important medicinal-ornamental Hymenocallis littoralis plants

Abstract

Hymenocallis littoralis locally known as Melong kecil is an ornamental plant traditionally believed to have medicinal properties. This study was done to identify the best concentration and combinations of plant growth regulators (PGRs) for the initiation and production of friable callus. Four (4) weeks old plant was used in this study. Bulb was dissected and cleaned (sterilized) using standard surface sterilization technique and then cultured in semi-solid Murashige and Skoog (MS) media with combinations of 6-Benzylaminopurine (BAP) and 2, 4-D hormones at different concentrationsranging from 2.25 to 22.50 µM. The best concentration for callus induction was determined by using percentage of callus induction. The optimum concentration for callus initiation was obtained by using 13.50 µM of 2, 4-D and 4.50 µM of BAP with 93.75% of callus induction rate. However, concentration 22.50 µM of 2, 4-D and 9.50 µM of BAP produced the lowest callus induction at 12.50%. The earliest time period, which is needed to initiate callus from the meristematic tissue was obtained at 15 days on semi-solid MS media with 13.50 µM of 2,4-D and 4.50 µM of BAP. In contrast, culture medium with 18.00 µM of 2, 4-D and 9.00 µM of BAP took 40 days from inoculation to produce callus. Culture medium containing 9.00, 13.50 and 18.00 µM of 2,4-D together with 4.50 µM of BAP produced yellow friable callus whereas the other concentrations produced yellow compact calluses. Hence, 13.50 µM of 2,4-D and 4.50 µM of BAP concentration is the best medium for callus induction for H. littoralis.

Direct regeneration and Genetic transformation studies in Hemidesmus indicus (L.) R. Br. (Indian Sarasaprilla)

“…1). In contrary to this similar combination with 13.50 µM 2, 4-D and 4.50 µM BAP resulted in 93.75% of callus induction percentage with yellow friable callus and was an ideal medium for callus induction in Hymenocallis littoralis (Jeevandran et al 2012). Similarly Manisha and Rajesh, (2013) also obtained maximum callus response of 91.2% on MS medium supplemented with 3.0 mg L -1 BAP and 0.5 mg L -1 2,4-D from leaf explants of Tecomella undulate (Sm.)…”

Effect of Abiotic and Biotic Elicitors on Callus and Suspension from Piper Betle L. Var. Nigra

“…However, a concentration of 22.50 M 2, 4-D, and 9.50 M BAP gave a percentage of callus induction of 12.50%. Callus induction time was 15 days, and longer when compared to treatment with a CoCl2 elicitor (Sundarasekar et al, 2012).…”