Preliminary studies of enhanced contrast radiography in anatomy and embryology of insects with Elettra synchrotron light

Hönnicke, Marcelo Gonçalves; Foerster, Luís A.; Navarro-Silva, Mário Antônio; Menk, R.H.; Rigon, Luigi; Cusatis, C.

doi:10.1016/j.nima.2005.03.091

Cited by 6 publications

(1 citation statement)

References 28 publications

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“…The use of MacroCT and MicroCT imaging for the non-invasive study of insects, collectively termed ‘diagnostic radioentomology’ (DR), is increasing (Hornschemeyer et al 2002; Johnson et al 2004; Hönnickea et al 2005; Greco et al 2005; Greco et al 2006; Greco et al 2008; Greco et al 2009, Greco et al 2011). Results from this study indicate that it is feasible to observe plasticity of the honey bee brain ‘in vivo’ using DR, and that progressive, real-time observations of these changes can be followed in individual live bees in association with environmental stimuli.…”

Section: Discussionmentioning

confidence: 99%

Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology

Greco

Tong

Soleimani

et al. 2012

Journal of Insect Science

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The sensitivity of the honey bee, Apis mellifera L. (Hymeonoptera: Apidae), brain volume and density to behavior (plasticity) makes it a great model for exploring the interactions between experience, behavior, and brain structure. Plasticity in the adult bee brain has been demonstrated in previous experiments. This experiment was conducted to identify the potentials and limitations of MicroCT (micro computed tomograpy) scanning “live” bees as a more comprehensive, non-invasive method for brain morphology and physiology. Bench-top and synchrotron MicroCT were used to scan live bees. For improved tissue differentiation, bees were fed and injected with radiographic contrast. Images of optic lobes, ocelli, antennal lobes, and mushroom bodies were visualized in 2D and 3D rendering modes. Scanning of live bees (for the first time) enabled minimally-invasive imaging of physiological processes such as passage of contrast from gut to haemolymph, and preliminary brain perfusion studies. The use of microCT scanning for studying insects (collectively termed ‘diagnostic radioentomology’, or DR) is increasing. Our results indicate that it is feasible to observe plasticity of the honey bee brain in vivo using diagnostic radioentomology, and that progressive, real-time observations of these changes can be followed in individual live bees. Limitations of live bee scanning, such as movement errors and poor tissue differentiation, were identified; however, there is great potential for in-vivo, non-invasive diagnostic radioentomology imaging of the honey bee for brain morphology and physiology.

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Section: Discussionmentioning

confidence: 99%

Imaging Live Bee Brains using Minimally-Invasive Diagnostic Radioentomology

Greco

Tong

Soleimani

et al. 2012

Journal of Insect Science

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Diffraction enhanced imaging and x‐ray fluorescence microtomography for analyzing biological samples

Rocha¹,

Pereira²,

Anjos³

et al. 2007

X-Ray Spectrometry

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In this work, breast tissue samples were investigated in order to verify the distribution of certain elements by x‐ray fluorescence computed tomography (XRFCT) correlated with the characteristics and pathology of each tissue observed by diffraction enhanced imaging (DEI). The DEI system can show details in low attenuation tissues. It is based on the contrast imaging obtained by extinction, diffraction and refraction characteristics and can improve reduction in false positive and false negative diagnoses. XRFCT allows mapping of all elements within the sample, since even a minute fluorescence signal can be detected. DEI imaging techniques revealed the complex structure of the disease, confirmed by the histological section, and showed microstructures in all planes of the sample. The XRFCT showed the distribution of Zn, Cu and Fe at higher concentration. Copyright © 2007 John Wiley & Sons, Ltd.

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