Preliminary studies of lipids of the trematodes Eurytrema pancreaticum, Calicophoron erschowi and the turbellarian Penecurva sibirica

Vykhrestyuk, Nadezhda P.; Yarygina, Galina V.

doi:10.1016/0166-6851(82)90031-7

Cited by 8 publications

(4 citation statements)

References 19 publications

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“…According to Barrett (1981), free sterols are predominant in helminths and probably one kind of steroid fraction is being esterified. The level of sterol component of P. sibirica, E. pancreaticum, and C. erschowi are 19.2, 43.6 and 68.6 % respectively (Vykhrestyuk and Yarygina 1982). This shows that the percentage of total sterol among the trematodes is not comparable.…”

Section: Discussionmentioning

confidence: 90%

“…It is known that helminths' eggs contain substantial amount of sterols (Smith et al 1977). Vykhrestyuk and Yarygina (1982) opined that the reason for high percentage of sterol accounts for the presence of higher number of eggs. In the present study, the trematodes were pooled from ten hosts and all the parasites were adults and were engorged with eggs.…”

Section: Discussionmentioning

confidence: 99%

“…Trematodes are unable to synthesize most essential fatty acids and they have to acquire exogenous lipids from their host. The amount of total lipids, phospholipids, neutral lipids and fatty acids was determined in cattle parasites, namely the trematodes Eurytrema pancreaticum, Calicophoron erschowi, and in the freeliving turbellarian Penecurva sibirica (Vykhrestyuk and Yarygina 1982). They observed neutral lipids of these flatworms contained sterols, sterol esters, triacylglycerol, and free fatty acids.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of fatty acid contents of the neutral and phospholipids of the trematode Paramphistomum cervi and liver of its host, Capra hircus

Ghosh¹,

Misra²

2013

J Parasit Dis

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The present observation records the lipid classes and their fatty acid composition of the neutral lipid and phospholipid of the trematode Paramphistomum cervi and liver of its host Capra hircus, the common Indian goat. Thin Layer Chromatography and Gas Liquid Chromatography were used to identify different neutral lipid and phospholipid components. The results show that among the neutral lipid fractions, the amount of combined hydrocarbon, wax ester and steryl ester is more or less equal in parasite and its host, but the percent of triacylglycerol is more in host liver than the parasite and the percent of total sterol is more in parasite than that of its host liver. Among the phospholipid fractions, phosphatidylinositol and phosphatidylethanolamine in the trematode parasite P. cervi and phosphatidylcholine and cardiolipin in the host's liver are the major components. The predominant fatty acids in the neutral lipid and phospholipid fractions of both the host liver and the trematode P. cervi include C16:0, C18:0, C18:1, and C18:2. The results reveal that the parasite P. cervi take up almost all the lipid and fatty acids from their host, which is required for their life cycle.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of fatty acid contents of the neutral and phospholipids of the trematode Paramphistomum cervi and liver of its host, Capra hircus

Ghosh¹,

Misra²

2013

J Parasit Dis

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“…The difference in lipid and their fractions in the developmental stages under study can be correlated with the metabolic and physiological differences of their microhabitat which are influenced by the effects of various environmental factors. Further, Vykhrestyuk and Yarygina [65] have suggested that the inconsistency in the lipid composition of parasites may depend on factors like parasite species, sex, age and environmental factors like habitat and diet of the host. Among the major lipid fractions in G. crumenifer developmental stages the phospholipids concentration was observed higher followed by cholesterol and free fatty acids.…”

Section: Gr Up Smmentioning

confidence: 99%

Biochemical Changes in the Rumen Infecting Paramphistome, Gastrothylax crumenifer during Miracidial and Intramolluscan Developmental Stages

Saifullah¹,

Ahmad²,

Abidi³

et al. 2016

SM Trop Med J

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We hypothesize that the level of various biomolecules is variable and remain in a dynamic state during the course of development in Gastrothylax crumenifer. The aim of the present study is to determine whether these molecules are in dynamic state or their levels are constant in all the developmental stages. The present study was designed to investigate level of various macromolecules e.g. protein, glycogen, lipid, lipid fractions and nucleic acids by standard Spectrophotometric methods. All the biomacromolecules are expressed in terms of mg/g wet weight. A minimum of three separate replicates were run for each experiment. To investigate similarities/ differences in polypeptides of different developmental stages namely fresh eggs, eggs containing mature miracidia (Em), cercariae, metacercariae, immature and adult stages, SDS-gradient PAGE was performed. In the present study we have investigated biochemical alterations during the larval stages of Gastrothylax crumenifer (a rumen infecting paramphistome) which revealed marked differences during the miracidial development as well as in the intra-molluscan larval stages. We observed that the protein, glycogen and lipid of freshly laid eggs were utilized during the development. Concentration of protein, glycogen and lipid contents significantly elevated in the cercariae as compared to the non-feeding metacercarial stage, indicating an adaptation of the parasite to build up the nutrients for the metacercariae which is a dormant stage. Very little amount of lipid was detected in the fresh eggs (E0) which further decreased in the eggs containing mature miracidia (Em) and then increased sharply in the cercarial and metacercarial stages indicating the substantial buildup of lipid reserves. Nucleic acid contents decreased from E0 to the subsequent developmental stages. Such changes could be of intrinsic significance for the cellular differentiation and organogenesis in larval paramphistomes. Further, we analyzed polypeptide profile of developmental stages which revealed heterogeneous mixture of polypeptides. A total of 27, 15, 14, 15, 14 and 34 polypeptides in E0, Em, cercariae, metacercariae, immature and adult G. crumenifer, respectively were resolved. Eight and nine characteristic polypeptides (17-90 kDa) were observed in E0 and adult stages respectively.

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Fatty acids of phospholipids from three digenetic trematodes of buffalo

Siddiqui

Siddiqi

1986

Z. Parasitenkd.

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Preliminary studies of lipids of the trematodes Eurytrema pancreaticum, Calicophoron erschowi and the turbellarian Penecurva sibirica

Cited by 8 publications

References 19 publications

Comparison of fatty acid contents of the neutral and phospholipids of the trematode Paramphistomum cervi and liver of its host, Capra hircus

Comparison of fatty acid contents of the neutral and phospholipids of the trematode Paramphistomum cervi and liver of its host, Capra hircus

Biochemical Changes in the Rumen Infecting Paramphistome, Gastrothylax crumenifer during Miracidial and Intramolluscan Developmental Stages

Fatty acids of phospholipids from three digenetic trematodes of buffalo

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