Preliminary studies on development of a novel subunit vaccine targeting Clostridium perfringens mucolytic enzymes for the control of necrotic enteritis in broilers

Avian Pathogenic Escherichia coli and Clostridium perfringens: Challenges in No Antibiotics Ever Broiler Production and Potential Solutions

“…Prevalence of cpb2 , netB , and tpeL was high in NE-producing isolates than non-NE producing isolates [ 152 ]. Genes netB and tpeL were present in human isolated C. perfringens , but presence may not correlate with the virulence of NE [ 154 ]. The gene cpb2 has not been associated with virulence in broiler chickens as cpb2 -positive isolates from diseased birds failed to produce the CPB2 toxin [ 150 , 155 ].…”

“…Recombinant protein vaccination in combination with Montanide™ ISA 71 VG adjuvant had significantly higher weight gain, and increased antibody titers when compared to the control challenged and adjuvant alone groups [ 153 ]. A preliminary study conducted by Duff et al identified five C. perfringens mucinase peptides that inhibited C. perfringens growth in vivo [ 154 ]. The peptides were then conjugated to an agonistic, CD40-targeting antibody and administered to live broilers challenged with C. perfringens and E. maxima .…”

“…Vaccination can be a promising alternative tool, especially if combinations of select antigens are pooled into a single vector. Future developments in NE reduction should include anti-clostridial vaccines focused on C. perfringens toxin peptides and Eimeria antigens into a single vector [ 154 ].…”

Avian Pathogenic Escherichia coli and Clostridium perfringens: Challenges in ‘No Antibiotics Ever’ Broiler Production and Potential Solutions

“…Prevalence of cpb2, netB, and tpeL was high in NE-producing isolates than non-NE producing isolates [159]. Genes netB and tpeL were present in human isolated C. perfringens, but presence may not correlate with the virulence of NE [177]. The gene cpb2 has not been associated with virulence in broiler chickens as cpb2-positive isolates from diseased birds failed to produce the CPB2 toxin [151,178].…”

“…Recombinant protein vaccination in combination with Montanide™ ISA 71 VG adjuvant had significantly higher weight gain, and increased antibody titers when compared to the control challenged and adjuvant alone groups [176]. A preliminary study conducted by Duff et al identified five C. perfringens mucinase peptides that inhibited C. perfringens growth in vivo [177]. The peptides were then conjugated to an agonistic, CD40-targeting antibody and administered to live broilers challenged with C. perfringens and Eimeria maxima.…”