Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from Sargassum fusiforme

Abstract

Abstract:In order to investigate the antioxidant properties of the polysaccharides from the brown alga Sargassum fusiforme, the crude polysaccharides from S. fusiforme (SFPS) were extracted in hot water, and the lipid peroxidation inhibition assay exhibited that SFPS possessed a potential antioxidant activity. Hence, two purely polymeric fractions, SFPS-1 and SFPS-2 were isolated by the column of DEAE (2-diethylaminoethanol)-Sepharose Fast Flow, with their molecular weights of 51.4 and 30.3 kDa determined by high performance gel permeation chromatography (HPGPC). They were preliminarily characterized using chemical analysis in combination of infrared (IR) and nuclear magnetic resonance (NMR) spectroscopies and found to contain large amounts of uronic acids and β-glycosidical linkages. The antioxidant activities of these two SFPS fractions were evaluated using superoxide and hydroxyl radical-scavenging assays. The results show that the antioxidant ability of SFPS-2 was higher than that of SFPS-1, probably correlating with the molecular weight and uronic acid content.

Aqueous fractionation yields chemically stable lupin protein isolates

“…Polysaccharides can have anti-oxidative properties as well, as reported for oil-in-water emulsions (Chen, Zhang, & Xie, 2004;Mateos-Aparicio, Mateos-Peinado, Jiménez-Escrig, & Rupérez, 2010;Wang et al, 2013;Waraho et al, 2011;Zhou, Hu, Wu, Pan, & Sun, 2008). The anti-oxidative mechanism is due to their metal-chelating activity, free radical scavenging activity, and additionally by the increase in viscosity (Waraho et al, 2011).…”

“…Since lupin has a higher total dietary fibre content and a higher soluble dietary fibre percentage than soybean (Bähr et al, 2014), the fibre-rich pellet may exhibit even higher antioxidative activity than the fibre-rich material from soybean. Additionally, uronic acids, present in pectin, are positively related with an improved oxidative stability of polysaccharides in tea and brown algae through radical-scavenging (Chen et al, 2004;Zhou et al, 2008). The cell walls of the lupin cotyledon contain a substantial amount of pectins (Guillon & Champ, 2002), and thus an improved oxidative stability of the fibre-rich pellet was to be expected.…”

Application of Celluclast 1.5L in apple pectin extraction

“…(2010); Zhou, Hu, Wu, Pan & Sun (2008) and Dou, Meng, Liu, Ren & Guo (2015). These researchers proved that there was a positive correlation between uronic acids content in pectin and its antioxidant activities.…”

In vitro and in vivo antioxidative and radioprotective capacities of polysaccharide isolated from Mesona Blumes gum

“…This indicated that the existence of uronic acids was the directive factor for the radical scavenging effects of tea polysaccharide conjugates. It also showed a positive correlation between uronic acids and antioxidant capacities in brown alga polysaccharides. The scavenging of the hydroxyl radical was important for antioxidant defence in food systems.…”