Preliminary studies on the production of high and low viscosity dextran by Leuconostoc spp.

Shamala, T. R.; Prasad, M.

doi:10.1016/0032-9592(95)85004-x

Cited by 39 publications

(10 citation statements)

References 10 publications

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“…With yields of extracted dextran comprising between 8 and 20 g/L [11,12], immobilization of beads was first studied with different concentrations of dextran. A concentration of 4 g/L corresponds to a BFI of 11.5 and to a BFI of 8.5 at a concentration of 50 g/L.…”

Section: Resultsmentioning

confidence: 99%

New Method Showing the Influence of Matrix Components in Leuconostoc mesenteroides Biofilm Formation

Badel

Laroche

Gardarin

et al. 2008

Appl Biochem Biotechnol

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Studying biofilm formation and influence of the matrix composition was heavy because only old and long methods were employed up to now: confocal microscopy, fluorescent chemical markers, and/or dying techniques. In this context, an innovative tool, the BioFilm Ring Test, was here employed to explore the role of exopolysaccharides, proteins, and nucleic acids in the formation of biofilm by Leuconostoc mesenteroides. The principle is to add magnetic particles in the culture medium. When a biofilm is formed, particles are unable to migrate in the media to form a ring when a magnet is brought nearer to the well. Therefore, culture media supplemented with proteases, glycanases, and/or nucleases allowed us to identify the involvement of these substances in L. mesenteroides biofilm formation. The results permitted to demonstrate that dextran, proteins, and nucleic acids are implied in biofilm formation.

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Section: Resultsmentioning

confidence: 99%

New Method Showing the Influence of Matrix Components in Leuconostoc mesenteroides Biofilm Formation

Badel

Laroche

Gardarin

et al. 2008

Appl Biochem Biotechnol

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“…Dextran solution (5%) was used as a stock solution for viscosity measurement at 25ºC using an Ostwald Viscometer 17.…”

Section: Methodsmentioning

confidence: 99%

Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713

Sarwat

Qader²,

Aman³

et al. 2008

Int. J. Biol. Sci.

141

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On the basis of high enzyme activity a newly isolated strain of L. mesenteroides CMG713 was selected for dextran production. For maximum yield of dextran, effects of various parameters such as pH, temperature, sucrose concentration and incubation period were studied. L. mesenteroides CMG713 produced maximum dextran after 20 hours of incubation at 30°C with 15% sucrose at pH 7.0. The molecular mass distribution of dextran produced by this strain showed that its molecular mass was about 2.0 million Da. Dextran analysis by 13 C-NMR spectrometry showed no signals corresponding to any other linkages except α-(1→6) glycosidic linkage in the main chain, which has not been reported before. Physico-chemical properties of this unique dextran were also studied. These optimised conditions could be used for the commercial production of this unique high molecular weight dextran, which have significant industrial perspectives.

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“…From sucrose, this enzyme catalyses the synthesis of a high molecular weight (HMW) polymer called dextran, in which more than 95% of the d ‐glucosyl units are α‐1,6 linked. Owing to its nonionic behaviour, biocompatibility and biodegradability, this dextran has found many commercial applications in the pharmaceutical field (plasma substitute, ironcarrier, anticoagulant) or in bioseparations, where it is used as chromatographic support (Sephadex ® gels) (Shamala & Prasa, 1995). Mainly because of its industrial applications, DSR‐S has become the most intensively studied enzyme from the GH family 70.…”

Section: Introductionmentioning

confidence: 99%

High-level production and purification of a fully active recombinant dextransucrase fromLeuconostoc mesenteroidesNRRL B-512F

Moulis

Arcache

Escalier

et al. 2006

FEMS Microbiology Letters

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Recombinant expression of the dextransucrase dsrS gene by Escherichia coli was optimized to produce 5850 U L(-1) (culture) of DSR-S, corresponding to a 30-fold increase compared with previous studies. Rational deletions of the signal peptide, the beginning of the variable region and the last four repeats of the C-terminal end caused no loss of activity. This new variant successfully purified was remarkably stable. With a k(cat) of 584 s(-1), it is the most efficient recombinant glucansucrase described to date. The synthesized polymer possesses more than 95% of alpha-1,6 links, like the dextran produced by the native enzyme, and innovative gel properties were obtained.

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Preliminary studies on the production of high and low viscosity dextran by Leuconostoc spp.

Cited by 39 publications

References 10 publications

New Method Showing the Influence of Matrix Components in Leuconostoc mesenteroides Biofilm Formation

New Method Showing the Influence of Matrix Components in Leuconostoc mesenteroides Biofilm Formation

Production & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713

High-level production and purification of a fully active recombinant dextransucrase fromLeuconostoc mesenteroidesNRRL B-512F

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