Preliminary study of the antioxidant properties of flowers and roots of Pyrostegia venusta (Ker Gawl) Miers

Abstract

BackgroundFree radical stress leads to tissue injury and can eventually to arthritis, atherosclerosis, diabetes mellitus, neurodegenerative diseases and carcinogenesis. Several studies are ongoing worldwide to find natural antioxidants of plant origin. We assessed the in-vitro antioxidant activities and screened the phytochemical constituents of methanolic extracts of Pyrostegia venusta (Ker Gawl) Miers.MethodsWe evaluated the antioxidant potential and phytochemical constituents of P. venusta using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2, 2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays. Gas chromatography-mass spectroscopy (GC-MS) studies were also undertaken to assess the phytochemical composition of the flower extracts.ResultsPhytochemical analyses revealed the presence of terpenoids, alkaloids, tannins, steroids, and saponins. The reducing ability of both extracts was in the range (in μm Fe(II)/g) of 112.49-3046.98 compared with butylated hydroxytoluene (BHT; 63.56 ± 2.62), catechin (972.02 ± 0.72 μm) and quercetin 3208.27 ± 31.29. A significant inhibitory effect of extracts of flowers (IC50 = 0.018 ± 0.69 mg/ml) and roots (IC50 = 0.026 ± 0.94 mg/ml) on ABTS free radicals was detected. The antioxidant activity of the extracts of flowers (95%) and roots (94%) on DPPH radicals was comparable with that of ascorbic acid (98.9%) and BHT (97.6%). GC-MS study revealed the presence of myoinositol, hexadecanoic acid, linoleic acid, palmitic acid and oleic acid in the flower extracts.ConclusionThese data suggest that P. venusta is a natural source of antioxidants. The extracts of flowers and roots of P. venusta contain significant amounts of phytochemicals with antioxidative properties and could serve as inhibitors or scavengers of free radicals. P. venusta could be exploited as a potential source for plant-based pharmaceutical products. These results could form a sound basis for further investigation in the potential discovery of new natural bioactive compounds.

Evaluation of anticandidal and antioxidant activities of phenolic compounds from Pyrostegia venusta (Ker Gawl.) Miers

“…Miers, family Bignoniaceae, is traditionally used to treat erysipelas as well as pulmonary and vaginal infections [9,10]. Compounds isolated from the extracts of this species present antioxidant and antimicrobial activities and elicit hyperpigmentation [11,12]. Veloso et al [13] have reported that this species is useful to treat flu and cold symptoms induced by lipopolysaccharides.…”

Antioxidant and anti-inflammatory activities of ethyl acetate extract of Gynura formosana (Kitam) leaves

“…Previous studies have suggested that inflammatory tissue damage may be due to the liberation of reactive oxygen species from the phagocytes that invade inflammation sites (29,30). DPPH and ABTS radical scavenging assays, based on electron transfer and hydrogen atom transfer reaction, are analytical methods for the reliable determination of antioxidant activities of biological samples in vitro (31). In this study, samples from the EAEG treatment groups exhibited significant DPPH and ABTS radical scavenging activities and these results suggested that free radicals scavenging activities contribute to the antioxidant effects of EAEG in vitro, which may be responsible for its anti-inflammatory activities (32).…”

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“…Antioxidant potential of extract was estimated by determining its DPPH radical scavenging capability by adopting the method of Roy et al [15] with minor modifications. The dried methanol extract was diluted from 0.02 mg/ml to 0.12 mg/ ml.…”