Preliminary study on fluorimetric detection of aflatoxins Q1, P1 and B1 using heptakis-di-O-methyl-β-cyclodextrin as post-column HPLC reagent

Vázquez, Beatriz; Fente, C.; Franco, Carlos; Cepeda, Alberto; Mahuzier, G.; Prognon, Patrice

doi:10.1039/a809150a

Cited by 17 publications

(13 citation statements)

References 13 publications

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“…Similarly to the direct methods reported in the previous paragraph, it was demonstrated that DM β-CD gave rise to the best gain in fluorescence as shown for the determination of B 1 and G 1 aflatoxins in food analysis [35] or more recently, for B 1 aflatoxin and its metabolites P 1 in urine [36] as illustrated in figure 3.…”

Section: Detection In Separation Methodsmentioning

confidence: 89%

Usefulness of cyclodextrins for detection in molecular fluorescence. Application to xenobiotics and drugs

Prognon¹,

Kasselouri²,

Desroches³

et al. 2000

Analusis

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Section: Detection In Separation Methodsmentioning

confidence: 89%

Usefulness of cyclodextrins for detection in molecular fluorescence. Application to xenobiotics and drugs

Prognon¹,

Kasselouri²,

Desroches³

et al. 2000

Analusis

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“…They are available commercially, and their physical and chemical properties have been described in the literature (14). These oligomers are able to include a large number of organic and inorganic species in their cavities, and in this work, excitation of the natural fluorescence of aflatoxins has been accomplished using a derivative of these molecules (2,8,16,17).Experimental results obtained in previous work (2, 16, 17) revealed that ␤-cyd and its methylated derivatives have excellent cavities for exciting the fluorescence responses of aflatoxins B 1 and G 1 through the formation of inclusion complexes. In this work, we have exploited this property for the direct visual measurement of aflatoxin production by A. flavus and A. parasiticus strains.…”

mentioning

confidence: 99%

“…Experimental results obtained in previous work (2,16,17) revealed that ␤-cyd and its methylated derivatives have excellent cavities for exciting the fluorescence responses of aflatoxins B 1 and G 1 through the formation of inclusion complexes. In this work, we have exploited this property for the direct visual measurement of aflatoxin production by A. flavus and A. parasiticus strains.…”

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confidence: 99%

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confidence: 99%

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New Additive for Culture Media for Rapid Identification of Aflatoxin-Producing Aspergillus Strains

Fente

Ordaz

Vázquez

et al. 2001

Appl Environ Microbiol

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102

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A new reliable, fast, and simple method for the detection of aflatoxigenic Aspergillus strains, consisting of the addition of a cyclodextrin (a methylated ␤-cyclodextrin derivative) to common media used for testing mycotoxin production ability, was developed. We propose the use of this compound as an additive for fungal culture media to enhance the natural fluorescence of aflatoxins. The production of aflatoxins coincided with the presence of a bright blue or blue-green fluorescent area surrounding colonies when observed under longwavelength (365-nm) UV light after 3 days of incubation at 28°C. The presence of aflatoxins was confirmed by extracting the medium with chloroform and examining the extracts by high-pressure liquid chromatography with fluorescence detection.Aflatoxins are mycotoxins with highly toxic and carcinogenic properties produced by some strains of Aspergillus flavus, Aspergillus parasiticus, and Aspergillus nomius. These fungi are frequently found in foodstuffs and animal feeds. However, not all strains are able to produce aflatoxins, and this has encouraged the use of screening for their aflatoxin production abilities. The methodology commonly used for this survey involves the culture of strains in a suitable liquid or solid medium and their later extraction and analysis for the presence of aflatoxins by chromatographic techniques. Yeast extract-sucrose (YES) medium (3) and natural media with wheat, rice, or peanut (9) have been used for this purpose. Testing large numbers of isolates on a variety of substrates with this procedure is tedious and time-consuming. For this reason, several screening methods for direct visual determination of aflatoxin production have been developed. These methods use more or less complicated culture media containing additives to enhance the production of aflatoxins in order to achieve direct visual determination of a bright blue or blue-green fluorescent area surrounding colonies under UV radiation. Thus, a complex agar medium containing sucrose, various salts, and an aqueous extract of aflatoxin-free peanuts (5); a modified Czapek agar medium containing corn steep liquor, named aflatoxin-producing ability (APA) medium (9); media containing coconut, named coconut agar medium (4, 13), coconut extract agar (11,12), and coconut cream agar (6); the synthetic liquid medium of Adye and Mateles (1); and a silica gel medium (15) are currently in use.The natural fluorescence of aflatoxins arises from their oxygenated pentaheterocyclic structure. The cyclodextrins (cyd) are molecules formed by the action of the enzyme cyd-transglycolase on dextrans and have different sizes [they contain from six to eight units of glucose in an ␣(1-4) configuration, according to which they are called ␣-, ␤-, or ␥-cyd]. They are available commercially, and their physical and chemical properties have been described in the literature (14). These oligomers are able to include a large number of organic and inorganic species in their cavities, and in this work, excitation of the natural fluorescen...

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“…The effects of cyclodextrins on substituted coumarins, such as the aflatoxins, have also been known for some time (Francis et al 1988, Vázquez et al 1991 and have been applied in several different ways. Among these include the use of CDs in fungal culture media as an aid in the identification of toxinproducing isolates (Fente et al 2001, Abbas et al 2004, Jaimez et al 2004, Rojas et al 2005, and the use of CDs as reagents in chemical analyses for the aflatoxins (Francis et al 1988, Cepeda et al 1996, Franco et al 1998, Vázquez et al 1999, Dall'Asta et al 2003. The latter include pre-column (Chiavaro et al 2001) and post-column (Francis et al 1988, Cepeda et al 1996, Vázquez et al 1999) HPLC assays.…”

Section: Applications Of Cds To the Analysis Of Mycotoxins Having A Nmentioning

confidence: 99%

Use of cyclodextrins as modifiers of fluorescence in the detection of mycotoxins

Maragos

Appell

Lippolis

et al. 2008

Food Additives & Contaminants: Part A

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Cyclodextrins, cyclic oligosaccharides composed of amylose subunits, are known to interact with mycotoxins. The interactions may be useful to analytical chemists by altering the properties of the mycotoxin of interest, namely the chromatographic properties, electrophoretic properties, fluorescence, or absorption of these fungal metabolites. Practical applications of these effects have been the incorporation of cyclodextrins into high-performance liquid chromatography and capillary electrophoresis methods for mycotoxin detection. Specific mycotoxins include those with a native fluorescence such as the aflatoxins, ochratoxin A (OTA) and zearalenone (ZEN) as well as those that can be rendered fluorescent through derivatization, such as T-2 toxin. The literature describing the applications of cyclodextrins in mycotoxin analysis is reviewed and an attempt to extend the use of cyclodextrins to the detection of labelled T-2 toxin is presented. Twenty cyclodextrins were evaluated for their ability to enhance the fluorescence emission of T-2 toxin derivatized with pyrene-1-carbonyl cyanide (T2-Pyr). This evaluation revealed that heptakis (2,6-di-O-methyl)--cyclodextrin (DIMEB), in particular, enhanced T2-Pyr fluorescence. DIMEB was used as a buffer modifier in a capillary electrophoresis-laser-induced fluorescence (CE-LIF) method for detecting T-2 in maize. Because of the effects that certain cyclodextrins have, especially under aqueous conditions, they may make useful additives for a variety of mycotoxin analytical methods.

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Preliminary study on fluorimetric detection of aflatoxins Q1, P1 and B1 using heptakis-di-O-methyl-β-cyclodextrin as post-column HPLC reagent

Cited by 17 publications

References 13 publications

Usefulness of cyclodextrins for detection in molecular fluorescence. Application to xenobiotics and drugs

Usefulness of cyclodextrins for detection in molecular fluorescence. Application to xenobiotics and drugs

New Additive for Culture Media for Rapid Identification of Aflatoxin-Producing Aspergillus Strains

Use of cyclodextrins as modifiers of fluorescence in the detection of mycotoxins

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