Preliminary study on haemocyte response to white spot syndrome virus infection in black tiger shrimp Penaeus monodon

Braak, C.B.T. van de; Botterblom, M.H.A.; Huisman, E.A.; Rombout, J.H.W.M.; Knaap, W.P.W. van der

doi:10.3354/dao051149

Cited by 87 publications

(47 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The low specificity for E2 enzymes by WSSV249 could also be one of the factors contributing to the broad host range observed with WSSV infections, though further experimental evidence is needed to prove this hypothesis. Similar to other in situ hybridization studies on WSSVinfected shrimps (9,16,52), expression of WSSV249 and PvUbc was detected in the epithelial lining of the stomach, subcuticular epidermis of the cephalothorax, and in aggregating hemocytes in the connective tissues. In a review by Lo and Kou (37), the epidermis and stomach are likely targets for WSSV infection, and the expression of viral genes could be detected in shrimp with low-level infection.…”

Section: Discussionsupporting

confidence: 64%

“…Crustaceans lack a true adaptive immune system and instead rely on an innate immune response, in which hemocytes are one of the cellular effectors employed by crustaceans in a defense against pathogenic invasion (1,22). As suggested by van de Braak et al (52), WSSV infection could have induced the aggregation of hemocytes to the sites of infection and may themselves be targets for WSSV infection (58). Our previous electron microscopy study also demonstrated that WSSV infects two types of shrimp hemocytes (55).…”

Section: Discussionmentioning

confidence: 88%

See 1 more Smart Citation

RING-H2 Protein WSSV249 from White Spot Syndrome Virus Sequesters a Shrimp Ubiquitin-Conjugating Enzyme, PvUbc, for Viral Pathogenesis

Wang

Chua

Gusti

et al. 2005

J Virol

View full text Add to dashboard Cite

Modification of proteins by ubiquitin is essential for numerous cellular processes. The RING-H2 finger motif has been implicated in ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Four proteins, WSSV199, WSSV222, WSSV249, and WSSV403, from white spot syndrome virus (WSSV) contain the RING-H2 motif. Here we report that WSSV249 physically interacts with a shrimp ubiquitin-conjugating enzyme, PvUbc, and mediates ubiquitination through its RING-H2 motif in the presence of E1 and PvUbc. Mutations of the putative zinc coordination residues in the RING-H2 domain of WSSV249, however, ablate ubiquitination efficiency. In addition, the RING-H2 domain of WSSV249 is capable of ubiquitination with UbcH1, UbcH2, UbcH5a, UbcH5b, UbcH5c, UbcH6, and UbcH10, respectively, exhibiting a low degree of E2 specificity. Significantly, the expression of WSSV249 and PvUbc increased during infection, as revealed by real-time PCR. Furthermore, in situ hybridization showed that WSSV249 and PvUbc display similar expression patterns in infected shrimps, and immunofluorescence and immunohistochemistry assays showed an increase of PvUbc in infected shrimp cells. These results suggest that the RING-H2 protein WSSV249 from WSSV may function as an E3 ligase via sequestration of PvUbc for viral pathogenesis in shrimp.

show abstract

Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 88%

RING-H2 Protein WSSV249 from White Spot Syndrome Virus Sequesters a Shrimp Ubiquitin-Conjugating Enzyme, PvUbc, for Viral Pathogenesis

Wang

Chua

Gusti

et al. 2005

J Virol

View full text Add to dashboard Cite

show abstract

“…WSSV-antigens that accumulate in the connective tissue attract haemocytes from circulation and subsequently degranulate rapidly [31]. This degranulation of haemocytes was detected with the WSH8 antibody that stains the content of granules [18].…”

Section: Haemocyte Reactionmentioning

confidence: 99%

“…It is also noteworthy that WSSV-antigens exiting the gut pass through the connective tissue, probably finding its way to the haemolymph that surrounds the external part of the gut, and subsequently may be transported to the lymphoid organ for their clearance [31,38].…”

Section: Haemocyte Reactionmentioning

confidence: 99%

Truncated VP28 as oral vaccine candidate against WSSV infection in shrimp: An uptake and processing study in the midgut of Penaeus monodon

Kulkarni

Rombout

Singh

et al. 2013

Fish & Shellfish Immunology

View full text Add to dashboard Cite

a b s t r a c tSeveral oral vaccination studies have been undertaken to evoke a better protection against white spot syndrome virus (WSSV), a major shrimp pathogen. Formalin-inactivated virus and WSSV envelope protein VP28 were suggested as candidate vaccine components, but their uptake mechanism upon oral delivery was not elucidated. In this study the fate of these components and of live WSSV, orally intubated to black tiger shrimp (Penaeus monodon) was investigated by immunohistochemistry, employing antibodies specific for VP28 and haemocytes. The midgut has been identified as the most prominent site of WSSV uptake and processing. The truncated recombinant VP28 (rec-VP28), formalin-inactivated virus (IVP) and live WSSV follow an identical uptake route suggested as receptor-mediated endocytosis that starts with adherence of luminal antigens at the apical layers of gut epithelium. Processing of internalized antigens is performed in endo-lysosomal compartments leading to formation of supra-nuclear vacuoles. However, the majority of WSSV-antigens escape these compartments and are transported to the inter-cellular space via transcytosis. Accumulation of the transcytosed antigens in the connective tissue initiates aggregation and degranulation of haemocytes. Finally the antigens exiting the midgut seem to reach the haemolymph. The nearly identical uptake pattern of the different WSSV-antigens suggests that receptors on the apical membrane of shrimp enterocytes recognize rec-VP28 efficiently. Hence the truncated VP28 can be considered suitable for oral vaccination, when the digestion in the foregut can be bypassed.

show abstract

“…The number of circulating hemocytes in various crustaceans has been observed to decrease after viral infection (20,21), or after challenge with bacterial components (34,39), but this leads to a rapid recovery in the circulating hemocyte level by the induction of hemocytic maturation in hematopoietic tissue and the release of new hemocytes into the circulation (32,34). From our results in P. monodon shrimp primary cell culture, the up-regulation of PmHHAP in WSSV-infected cultured hemocytes (Fig.…”

Section: Discussionmentioning

confidence: 66%

A Novel Viral Responsive Protein Is Involved in Hemocyte Homeostasis in the Black Tiger Shrimp, Penaeus monodon

Prapavorarat

Vatanavicharn

Söderhäll

et al. 2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

A novel viral responsive protein, namely hemocyte homeostasis-associated protein (HHAP), was characterized for its role in the response of shrimp to white spot syndrome virus infection. The full-length cDNAs of HHAP from the black tiger shrimp (PmHHAP), Penaeus monodon, and the fresh water crayfish (PlHHAP), Pacifastacus leniusculus, were obtained and showed high sequence identity to a hypothetical protein from various organisms, with the highest identity to the hypothetical protein TcasGA2_TC006773 from the red flour beetle, Tribolium castaneum (54% amino acid sequence identity). Transcripts of PmHHAP were expressed in various shrimp tissues with the highest expression in hematopoietic tissue, whereas the transcripts of PlHHAP were found in the hematopoietic and nerve tissues. Upon white spot syndrome virus infection, a high upregulation level of shrimp hemocytic HHAP mRNA and protein was observed by real-time reverse transcription-PCR and immunofluorescence microscopy, respectively. Gene silencing of PmHHAP by RNA interference resulted in a significant decrease in the number of circulating hemocytes and 100% shrimp mortality within 30 h of the double-stranded PmHHAP RNA injection (but not in control shrimp), indicating that HHAP is essential for shrimp survival. Interestingly, severe damage of hemocytes was observed in vivo in the PmHHAP knockdown shrimp and in vitro in shrimp primary hemocyte cell culture, suggesting that PmHHAP plays an important role in hemocyte homeostasis. Thus, it is speculated that the up-regulation of PmHHAP is an important mechanism to control circulating hemocyte levels in crustaceans during viral infection.

show abstract

Preliminary study on haemocyte response to white spot syndrome virus infection in black tiger shrimp Penaeus monodon

Cited by 87 publications

References 34 publications

RING-H2 Protein WSSV249 from White Spot Syndrome Virus Sequesters a Shrimp Ubiquitin-Conjugating Enzyme, PvUbc, for Viral Pathogenesis

RING-H2 Protein WSSV249 from White Spot Syndrome Virus Sequesters a Shrimp Ubiquitin-Conjugating Enzyme, PvUbc, for Viral Pathogenesis

Truncated VP28 as oral vaccine candidate against WSSV infection in shrimp: An uptake and processing study in the midgut of Penaeus monodon

A Novel Viral Responsive Protein Is Involved in Hemocyte Homeostasis in the Black Tiger Shrimp, Penaeus monodon

Contact Info

Product

Resources

About