Premeiotic DNA synthesis in fission yeast

Egel, Richard; Egel-Mitani, Michiko

doi:10.1016/0014-4827(74)90626-0

Cited by 249 publications

(181 citation statements)

References 10 publications

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“…MCB has been suggested to be the recognition sequence for ResI -CdclO in S.pombe (Caligiuri and Beach, 1993 Table IV. Media were prepared as described (Egel and Egel-Mitani, 1974;Gutz et al, 1974;Nurse, 1975;Moreno et al, 1990;Okazaki et al, 1990). …”

Section: Discussionmentioning

confidence: 99%

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

Miyamoto¹,

Tanaka²,

Okayama³

1994

The EMBO Journal

135

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In the fission yeast Schizosaccharomyces pombe, the cdc10+ and res1+ genes play a crucial role in the start of mitotic and meiotic cycles. They encode structurally related transcriptional complex proteins and regulate some S phase‐specific genes. Here we report the identification of a new member of this family named as res2+. res2+ has been isolated as a multicopy suppressor of a res1‐ null mutant and specifies a 73 kDa protein, which has two copies of the Swi/ankyrin motif and shares the highest sequence and structure similarity with the Res1 protein. res2+ is largely redundant in function with res1+ and is required for the initiation of mitotic and premeiotic DNA synthesis, but has an additional role in meiotic division. Unlike res1+, res2+ is highly induced during conjugation and strongly depends on cdc10+ for its activity. We conclude that the fission yeast contains two functionally overlapping parallel ‘start’ systems, Res1‐Cdc10 and Res2‐Cdc10, the former of which plays a major role in mitotic cycle whereas the latter in meiotic cycle.

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Section: Discussionmentioning

confidence: 99%

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

Miyamoto¹,

Tanaka²,

Okayama³

1994

The EMBO Journal

135

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“…The media used have been described previously (Egel and Egel-Mitani, 1974;Gutz et al, 1974;Moreno et al, 1990). S. pombe cells were grown at 30°C and sporulated at 28°C.…”

Section: Yeast Strains Media and Transformationmentioning

confidence: 99%

“…Schizosaccharomyces pombe cells initiate a sporulation program when challenged by nutrient starvation, particularly starvation for nitrogen (Egel and Egel-Mitani, 1974;Yamamoto et al, 1997). Sporulation consists of two overlapping processes, meiosis and spore morphogenesis.…”

Section: Introductionmentioning

confidence: 99%

Live Observation of Forespore Membrane Formation in Fission Yeast

Nakamura

Asakawa

Nakase

et al. 2008

MBoC

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Sporulation in the fission yeast Schizosaccharomyces pombe is a unique biological process in that the plasma membrane of daughter cells is assembled de novo within the mother cell cytoplasm. A double unit membrane called the forespore membrane (FSM) is constructed dynamically during meiosis. To obtain a dynamic view of FSM formation, we visualized FSM in living cells by using green fluorescent protein fused with Psy1, an FSM-resident protein, together with the nucleus or microtubules. The assembly of FSM initiates in prophase II, and four FSMs in a cell expand in a synchronous manner at the same rate throughout meiosis II. After the meiosis II completes, FSMs continue to expand until closure to form the prespore, a spore precursor. Prespores are initially ellipsoidal, and eventually become spheres. FSM formation was also observed in the sporulation-deficient mutants spo3, spo14, and spo15. In the spo15 mutant, the initiation of FSM formation was completely blocked. In the spo3 mutant, the FSM expanded normally during early meiosis II, but it was severely inhibited during late and postmeiosis, whereas in the spo14 mutant, membrane expansion was more severely inhibited throughout meiosis II. These observations suggest that FSM expansion is composed of two steps, early meiotic FSM expansion and late and post meiotic FSM expansion. Possible regulatory mechanisms of FSM formation in fission yeast are discussed.

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“…However, if haploid cells of the two mating types (h and h ÿ ) meet under nitrogen-starved conditions, they enter the pathway for sexual development. They arrest transiently at G1, mate to form diploid zygotes, and then undergo meiosis and sporulation (Egel 1973;Egel & Egel-Mitani 1974). Furthermore, the mating pheromones M-factor and Pfactor have the ability to arrest the cell cycle of the mating partner at G1 phase (Davey & Nielsen 1994;.…”

Section: Introductionmentioning

confidence: 99%

Schizosaccharomyces pombe gad7⁺ encodes a phosphoprotein with a bZIP domain, which is required for proper G1 arrest and gene expression under nitrogen starvation

et al. 1996

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Premeiotic DNA synthesis in fission yeast

Cited by 249 publications

References 10 publications

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

Live Observation of Forespore Membrane Formation in Fission Yeast

Schizosaccharomyces pombe gad7⁺ encodes a phosphoprotein with a bZIP domain, which is required for proper G1 arrest and gene expression under nitrogen starvation

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Premeiotic DNA synthesis in fission yeast

Cited by 249 publications

References 10 publications

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

res2+, a new member of the cdc10+/SWI4 family, controls the ‘start’ of mitotic and meiotic cycles in fission yeast.

Live Observation of Forespore Membrane Formation in Fission Yeast

Schizosaccharomyces pombe gad7+ encodes a phosphoprotein with a bZIP domain, which is required for proper G1 arrest and gene expression under nitrogen starvation

Contact Info

Product

Resources

About

Schizosaccharomyces pombe gad7⁺ encodes a phosphoprotein with a bZIP domain, which is required for proper G1 arrest and gene expression under nitrogen starvation