Prenatal and newborn paternity testing with DNA analysis

Csete, Klára; Beer, Zs.; Varga, Tibor V.

doi:10.1016/j.forsciint.2004.09.101

Cited by 7 publications

(2 citation statements)

References 8 publications

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“…The present study has shown that a battery of 15 polymorphic STR loci offers a discriminating power sufficient to exclude or include an alleged father in disputed paternity cases. This is consistent with many reports which described the usefulness and discrimination power of STR markers [35,20,15,11]. In paternity and identity testing, the allele frequency database obtained from the population to which the person in question belongs should be used in the calculations for forensic evaluations.…”

Section: Discussionsupporting

confidence: 87%

Paternity testing and forensic DNA typing by multiplex STR analysis using ABI PRISM 310 Genetic Analyzer

El-Alfy

El-Hafez²

2012

Journal of Genetic Engineering and Biotechnology

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Short tandem repeats (STRs) are widespread throughout the human genome and are a rich source of highly polymorphic markers which can be detected by PCR. To gain a better appreciation for how the polymorphism at a particular locus impacts the individual identity, the present study was undertaken to explore the use of 15 STR loci in forensic investigation and paternity testing. Multiplex STR typing was used to study the 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA) in addition to a gender identification marker, amelogenin, by capillary electrophoresis on 310 Genetic Analyzer. Samples from 85 trio and duo cases of disputed paternity were investigated. The data were analyzed to give information on paternity index, probability of paternity, frequency of number of exclusions and rate of mismatch at each STR locus. The method was also successfully applied to forensic personal identification in theft and murder cases. The results demonstrated that the STR typing is a reliable and robust tool for analyzing the forensic practice as well as for paternity testing. The advantages of using multiplex STR analysis over other conventional methods are discussed.

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Section: Discussionsupporting

confidence: 87%

Paternity testing and forensic DNA typing by multiplex STR analysis using ABI PRISM 310 Genetic Analyzer

El-Alfy

El-Hafez²

2012

Journal of Genetic Engineering and Biotechnology

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“…Consequently, a compulsory part of the autopsy is the examination of the uterus, or in the case where pregnancy is detected, the securing of a biological sample linked to the fetus. This sample may be-depending on the age of the fetus and the postmortem interval-from uterine wall scrapings, placenta, the fetus, or any tissue, organ, blood, umbilical cord, bone, or any other fetal internal organs which contain the DNA of the terminated fetus [23]. In the case of uterine wall scrapings, avoiding the mixture of the mother-fetus samples requires special care, especially in the case of family-genetically related-violence since the mixed genetic profile can influence the statistical interpretation of putative fatherhood [24,25].…”

Section: Introductionmentioning

confidence: 99%

Most Common Medico-Legal Autopsy-Related Human and Nonhuman Biological Samples for DNA Analysis

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The identification and individualization of biological evidences is crucial to actual criminal investigations. In spite of the differences at the national level, all the legal processes attribute particular importance to forensic DNA analysis. However, none of the qualified results from any professional laboratory can produce substantive, valuable evidence with insufficient quality of samples and/or problems with provision of a pristine and controlled environment. The methodology and efficiency of sampling are distinct in case of living persons and in medico-legal autopsy and crime scenes. This chapter is a short overview from the basic introductory information up to ongoing research, and in accordance with constraints on the chapter size, it briefly discusses the important topics of sample collection at medico-legal autopsy for DNA analysis. The content sorts the major types of samples, reviews the common methods of sampling and the potential risk of poor sampling or contamination transfer. The corpses can be more or less degraded, which in special cases (e.g., paraffin embedded tissues, drowned, burning and/or buried cadaver) allow only for analysis of highly degraded samples. The samples can be associated with tissues of a corpse (e.g., blood, soft tissues, bone, tooth, hair) and/or additional extraneous tissues and remains, which are often mixed (e.g., blood, saliva, semen, vaginal fluid, debris of fingernails) on the corpse.

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Forensic Aspects of Perinatal Pathology

Malcomson

2022

Keeling's Fetal and Neonatal Pathology

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Prenatal and newborn paternity testing with DNA analysis

Cited by 7 publications

References 8 publications

Paternity testing and forensic DNA typing by multiplex STR analysis using ABI PRISM 310 Genetic Analyzer

Paternity testing and forensic DNA typing by multiplex STR analysis using ABI PRISM 310 Genetic Analyzer

Most Common Medico-Legal Autopsy-Related Human and Nonhuman Biological Samples for DNA Analysis

Forensic Aspects of Perinatal Pathology

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