Prenatal progesterone exposure of male rats induces morphometric and histological changes in testes

Ahmed, Samy Ismail; Elsheikh, Adil Salim; Attia, Gamal Ali; Ali, Tahir Osman

doi:10.1016/j.apjr.2016.04.015

Cited by 9 publications

(11 citation statements)

References 32 publications

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“…In obstetrics, the hormone is used for the treatment of abnormal uterine bleeding, threatened miscarriages during the first trimester, primary infertility cases, preterm delivery prevention, and to prevent and treat abnormal endometrial thickening (endometrial hyperplasia). Nonobstetric uses include treatment of mammary pain in women with noncancerous breast disease and as a topical treatment in certain skin diseases [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

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Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

Ahmed

Magzoub

Al-Ayed

et al. 2020

International Journal of Endocrinology

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This study was designed to investigate the effect of prenatal exposure to synthetic sex steroid on sperm quantity and quality, relative testicular and epididymal weights, and reproductive hormones level in adult Wistar rats. Forty male Wistar rats were divided into two groups: a test group (n = 20) that included mature rats that were born to dams exposed to gestational treatment with hydroxyprogesterone and a control group (n = 20) that included mature rats born to untreated dams. Compared to the control group, the test group showed a significant reduction in the sperm count, viability and motility, relative testicular and epididymal weights together with increased abnormal spermatozoa (p<0.001). The reproductive hormonal assay revealed significantly lower serum testosterone and higher levels of FSH and LH among the test groups compared to the control (p<0.05 for all). Prenatal exposure to synthetic progesterone negatively affected sperm production and function, relative testicular and epididymal weights, and reproductive hormone levels.

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Section: Introductionmentioning

confidence: 99%

“…Following exposure to hydroxyprogesterone, recent evidence revealed abnormal morphometric and histological changes, including a reduction in sperm count, damaged seminiferous tubules, and decreased testicular and epididymal weights [7,8,11].…”

Section: Introductionmentioning

confidence: 99%

Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

Ahmed

Magzoub

Al-Ayed

et al. 2020

International Journal of Endocrinology

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“…The dam body weights were taken on each day of injection to adjust the dose. The 40 male rat pups were allowed to grow for 90 days where they reach maturity [2,4,[10][11][12].…”

Section: Methodsmentioning

confidence: 99%

“…The effects of these synthetic hormones on the reproductive functions of the male human and animal cause considerable concerns among researchers who care to improve the male reproductive health [9][10][11][12]. Abnormal changes in the male reproductive health such as abnormal sexual development, alteration in testicular functions and increment of infertility rates were suggested by current studies [10,[12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…The dam body weights were taken on each day of injection to adjust the dose. The 40 male rat pups were allowed to grow for 90 days where they reach maturity [2,4,[10][11][12].Study design: This one factorial experiment study was designed to investigate the effects of lactational exposure to DMPA on suckling male rat's pups' testis histological structure, morphometry, cells quantities at Citation: Ahmed SI, Ali TO, Elsheikh AS, Attia GA, Abdalla AM, et al (2017) Testicular Changes in Male Albino Rat Pups Exposed to MedroxyProgesterone Acetate during Lactational Period. J Steroids Horm Sci 8: 184.…”

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confidence: 99%

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Testicular Changes in Male Albino Rat Pups Exposed to Medroxy- Progesterone Acetate during Lactational Period

Ahmed¹,

To²,

As³

et al. 2017

J Steroids Horm Sci

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Citation: Ahmed SI, Ali TO, Elsheikh AS, Attia GA, Abdalla AM, et al. (2017) AbstractThere are great concerns regarding the use of synthetic progesterone during breastfeeding due to probable negative effects on future fecundity of male infants. Therefore the present study was conducted to evaluate the effects of exposing male rat pups to depot medroxyprogesterone acetate (DMPA) during lactational period on pubertal testicular histology, morphometry and cells quantitation. Twenty male Wistar rat pups reared to dams treated with DMPA (10 mg/ kg BW) every other day during their early lactation period; were employed to achieve the objectives of this study. Other 20 male rat pups reared to untreated dams served as control. The pups were allowed to reach 90 days old, sacrificed, their testes were dissected and weighed and histological sections were prepared.The results showed that exposing male rat pups to DMPA during the lactational period significantly (P<0.001) affected their testicular histology, morphometry and the quantities of testicular cells. The thickness of the germinal epithelium (GE) and the diameter of the seminiferous tubules (ST) were reduced; while the interstitial space (IS) thicknesses were increased. The testicular cells of the rats reared on dams treated with DMPA experienced varying degrees of apoptosis and count reduction. The ST appeared with unusual configuration with detached and/or folded basal lamina, it has few germinal layers, decreased Sertoli cells (SC) and their lumen contained cells debris and very few sperms; while the wide IS contained few Leydig cells (LC). Exposure to DMPA during the lactational period adversely affects the testicular structure. Thus foretells a negative impact on future fertility. Materials and Methods MaterialsExperimental animals: Ten weeks old 8 pregnant female Wistar albino rats were grouped into two groups (4 rats each) and kept separate away from any stress in sterilized polypropylene cages (90 cm × 45 cm × 15 cm) lined with woody husk. They were kept at 28 ± 7°C temperature in light/dark cycle (12:12 h), fed on commercial pellet and offered water ad libitum. After delivery 4 dams with 20 male pups (Group I) were injected with a placebo to serve as controls; while 4 dams with 20 male pups (Group II) were subcutaneously injected with 10 mg/kg BW of depot medroxy-progesterone acetate (DMPA; Depo-Provera®) every other day (on the first fifteen days) of lactational period. The dam body weights were taken on each day of injection to adjust the dose. The 40 male rat pups were allowed to grow for 90 days where they reach maturity [2,4,[10][11][12].Study design: This one factorial experiment study was designed to investigate the effects of lactational exposure to DMPA on suckling male rat's pups' testis histological structure, morphometry, cells quantities at Citation: Ahmed SI, Ali TO, Elsheikh AS, Attia GA, Abdalla AM, et al. (2017) Testicular Changes in Male Albino Rat Pups Exposed to MedroxyProgesterone Acetate during Lactational Period. J Steroids Horm Sc...

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Assessment of reproductive toxicity of gold nanoparticles and its reversibility in male albino rats

Abdulhaq,

Elnady,

Abo El-atta

et al. 2023

Toxicol Res.

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Nanotechnology has become a trending area in science all over the world. Although gold nanoparticles (AuNPs) have been utilized widely in biomedical fields, potential toxicities may arise from their interactions with biological systems. The current study aimed at evaluating the toxic effects of AuNPs on the reproductive system of adult male albino rats and assessing the recovery probability. In this study, AuNPs (13 ± 4 nm in diameter) were synthesized, and the experimental work was conducted on 60 adult male albino rats divided into the following groups: control group (received deionized water daily intraperitoneally (IP) for 28 days), test group, and withdrawal groups I and II (received 570 μg/kg of 13 ± 4 nm AuNPs daily IP for 28 days). Withdrawal groups I and II were left for another 30 and 60 days without sacrification, respectively. The test group showed significant decreases in final body and absolute testicular weights, testosterone hormone level, sperm count and motility, and spermatogenesis score, as well as significant increase in the percentage of sperms of abnormal morphology compared to the control group, associated with significant light and electron microscopic histopathological changes. Partial improvement of all studied reproductive parameters was detected after one month of withdrawal in withdrawal group I, and significant improvement and reversibility of all these parameters were reported after two months of withdrawal in withdrawal group II. So, AuNPs induce male reproductive toxicity, which partially improves after one month of withdrawal and significantly improves and reverses after two months of withdrawal.

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Prenatal progesterone exposure of male rats induces morphometric and histological changes in testes

Cited by 9 publications

References 32 publications

Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

Testicular Changes in Male Albino Rat Pups Exposed to Medroxy- Progesterone Acetate during Lactational Period

Assessment of reproductive toxicity of gold nanoparticles and its reversibility in male albino rats

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