Preoperative cobalt60 irradiation delays the healing of rectal anastomoses in rats

Abstract

The healing of colorectal anastomoses after irradiation therapy continues to be a major concern. The authors evaluated the healing of rectal anastomoses in a rat model after a preoperative 500-cGy dose of cobalt 60 irradiation. Thirty-six male Wistar rats were divided into two equal groups: control (group A), and irradiation group (group B). Group B received a single 500-cGy dose of irradiation, and a rectal resection and end-to-end anastomosis was performed in both groups on the 7th day after irradiation. Parameters of the healing process included bursting pressure and collagen content on the 5th, 7th, and 14th days after surgery. In the irradiation group, the mean bursting pressure on the 5th, 7th, and 14th days was 116, 218, and 273 mmHg, respectively. The collagen content assessed by histomorphometry was 9.0, 20.8, and 32%, respectively. In contrast, the control group had a mean bursting pressure of 175, 225 and 263 mmHg, and a collagen content of 17.8, 28.1, and 32.1%, respectively. The adverse effect of irradiation on healing was detectable only on the 5th postoperative day, as demonstrated by lower bursting pressure (P < 0.013) and collagen content (P < 0.008). However, there was no failure of anastomotic healing such as leakage or dehiscence due to irradiation. We conclude that a single preoperative 500-cGy dose of irradiation delays the healing of rectal anastomosis in rats.

Improvement of the breaking strength of wound by combined treatment with recombinant human G‐CSF, recombinant human M‐CSF, and a TGF‐β1 receptor kinase inhibitor in rat skin

“…( 7,8 ) Previous studies have demonstrated impaired wound healing and the development of fibrosis after irradiation in both patients and animal models. ( 8,9 ) Although the TGF‐b signal is essential for cutaneous wound healing, ( 10 ) radiation‐induced increases in the TGF‐b signal promote fibrosis and retard cutaneous wound healing. ( 11,12 ) Wound healing in irradiated skin has been reported to be accelerated in the Smad3‐deficient mouse in comparison with the wild‐type mouse, ( 13 ) suggesting that excessive activation of the TGF‐b signal may suppress wound healing in irradiated tissues.…”

Ginsenoside rich fraction of Panax ginseng C.A. Meyer improve feeding behavior following radiation-induced pica in rats

“…The group of rats treated with KG showed moderate positive staining for Masson trichrome, compared with the pica group. Consistently, gastric distention and motility are triggered with decreased collagen content in the submucosal region [35,36]. However, the association between acute emesis and collagen content upon KG treatment requires further detailed investigation.…”

Decellularized gastric matrix as a mesh for gastric perforation repair

“…Cobalt‐60 irradiation is a common sterilization method for biological materials. Transmission electron microscopy showed that sterilization with 12 kGy Cobalt‐60 irradiation did not significantly change the ultrastructure or the biomechanical strength of the PDGM and IDGM meshes 33 . CCK8 determined that the leachate through the PDGM mesh showed no significant cytotoxicity.…”