Preoperative transarterial chemoembolization for laparoscopic liver resection in Child A cirrhotic patients with hepatocellular carcinoma

López-López, Víctor; Brusadín, Roberto; López-Conesa, Asunción; Capel, Antonio; Navarro-Barrios, Álvaro; Cayuela, V.; Lopez-Banet, Elena; Garzón-Arana, Alejandro; Parrilla-Paricio, Pascual; Robles, R

doi:10.1007/s00423-020-02056-x

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…Data showed the two submitted sample profiles are from human, but not a match for any profile in the DSMZ STR database. The DNA typing results support that the two submitted samples are from the same individual with a likelihood ratio of 3.3868×10 21 . In other words, the tested cells are newly established.…”

Section: Successful Establishment Of Phhsmentioning

confidence: 52%

“…Some scholars believed that liver cancer patients who were resected after preoperative would have better long-term survival. 21 However, there were also report found preoperative TACE did not improve the patient’s survival. 22 These reports give support to our results.…”

Section: Discussionmentioning

confidence: 97%

“…However, differences in the genotypes of three loci between the PHH and its matched tissue were observed. Interestingly, the genotypes of the three loci were D13S317 (8,13) in the cell, D13S317(8, -) in the tissue, D18S51 (12,16) in the cell, D18S51 (12, -) in the tissue, FGA (21,27) in the cell, and FGA (21, -) in the tissue. This is a good demonstration of the tumor heterogeneity: genotypes showed differences in some loci between the tissue used for STR detection and the tissue used to establish PHHs.…”

Section: Successful Establishment Of Phhsmentioning

confidence: 99%

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New and effective method to develop primary hepatocytes from liver cancer patients

Wang

Fan

et al. 2022

Exp Biol Med (Maywood)

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Liver cancer (LC) is one of the most common malignant tumors worldwide. Since the mechanism of LC pathogenesis and metastasis cannot be carried out directly on the human body, it is particularly important to establish human liver cancer cell lines for research in vitro. In this study, tissue block adherence method combined with cell clumps digestion method was used to establish primary human hepatocytes (PHHs) with a successful rate of 60% (45/75). Short tandem repeat (STR) analysis proved the cells were derived from its paired tissues. These cells from hepatocellular carcinoma (HCC) expressed NTCP and secreted ALB and AAT as detected by western blot, and expressed hepatocyte-specific membrane protein ASGR1 as detected by flow cytometry. Liver cancer biomarkers like CK7 in ICC (intrahepatic cholangiocarcinoma), AFP, and GPC3 in HCC expressed of different degree as detected by immunohistochemical analysis. These cells displayed typical liver cancer cell morphological characteristics and can passage stably. In conclusion, we developed an effective method to establish PHHs. Further studies are necessary to study if these cells maintaining other liver function and reproduce the physiology of the tumors and how these cells behavior in the drug development.

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Section: Successful Establishment Of Phhsmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 97%

Section: Successful Establishment Of Phhsmentioning

confidence: 99%

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New and effective method to develop primary hepatocytes from liver cancer patients

Wang

Fan

et al. 2022

Exp Biol Med (Maywood)

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“…TACE is the first-line treatment for patients with intermediate-stage HCC. 3 It has been recently reported that patients in their early stages of TACE-based treatment could potentially benefit from the method, 26 , 27 resulting in an improved survival rate. However, complete embolism could only be observed in approximately 20% of the patients with HCC who were subjected to conditions of TACE.…”

Section: Discussionmentioning

confidence: 99%

Effect of Transarterial Chemoembolization Plus Percutaneous Ethanol Injection or Radiofrequency Ablation for Liver Tumors

Chen¹,

Zhang²,

Sun³

et al. 2022

JHC

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Background The efficacy of the transarterial chemoembolization (TACE) process combined with percutaneous ethanol injection (PEI, TACE-P) or the radiofrequency ablation (RFA, TACE-R) process was found to be good when used for the treatment of patients suffering from early or intermediate hepatocellular carcinoma (eiHCC). The study was conducted to compare the efficacy and safety of the TACE-P with TACE-A processes followed during the treatment of patients with eiHCC. Methods A total of 241 patients suffering from eiHCC, subjected to TACE-P (147 patients) or TACE-R (94 patients) processes from January 1, 2014, to December 31, 2018, were retrospectively reviewed and included. The propensity score matching (PSM) method was used to reduce selection bias. Results The median overall survival (mOS) and progression-free survival (mPFS) of the TACE-P group were similar to those recorded for the TACE-R group ( P >0.05) before using the PSM technique. Similar results were obtained post the use of the PSM technique. In the subgroup analysis after PSM, patients with single tumor (dimension: ≤5 cm), who were subjected to TACE-P-based treatment methods, exhibited worse tumor response than patients subjected to TACE-R-based methods (HR: 1.804, 95% CI: 1.083–3.005, P =0.023). Seven adverse events were reported. A statistically significant difference for all grades of adverse events (and grade III or IV adverse events) between the two groups (all P >0.05) was not reported. Conclusion The benefits and advantages of using the TACE-P based method was similar was those obtained using the TACE-R in patients with eiHCC, especially for patients with a single large tumor or multiple tumors.

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“…e final segmentation result was selected manually, but the effect was not good. Many relevant studies suggested that although HCC lesion segmentation is realized based on traditional machine learning algorithm, its high dependence on specified tasks and nonautomatic disadvantages cannot be ignored [9,10].…”

Section: Introductionmentioning

confidence: 99%

Automatic Segmentation Algorithm of Magnetic Resonance Image in Diagnosis of Liver Cancer Patients under Deep Convolutional Neural Network

Zhang

Zhao

2021

Scientific Programming

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To study the influence of different sequences of magnetic resonance imaging (MRI) images on the segmentation of hepatocellular carcinoma (HCC) lesions, the U-Net was improved. Moreover, deep fusion network (DFN), data enhancement strategy, and random data (RD) strategy were introduced, and a multisequence MRI image segmentation algorithm based on DFN was proposed. The segmentation experiments of single-sequence MRI image and multisequence MRI image were designed, and the segmentation result of single-sequence MRI image was compared with those of convolutional neural network (FCN) algorithm. In addition, RD experiment and single-input experiment were also designed. It was found that the sensitivity (0.595 ± 0.145) and DSC (0.587 ± 0.113) obtained by improved U-Net were significantly higher than the sensitivity (0.405 ± 0.098) and DSC (0.468 ± 0.115, P < 0.05 ) obtained by U-Net. The sensitivity of multisequence MRI image segmentation algorithm based on DFN (0.779 ± 0.015) was significantly higher than that of FCN algorithm (0.604 ± 0.056, P < 0.05 ). The multisequence MRI image segmentation algorithm based on the DFN had higher indicators for liver cancer lesions than those of the improved U-Net. When RD was added, it not only increased the DSC of the single-sequence network enhanced by the hepatocyte-specific magnetic resonance contrast agent (Gd-EOB-DTPA) by 1% but also increased the DSC of the multisequence MRI image segmentation algorithm based on DFN by 7.6%. In short, the improved U-Net can significantly improve the recognition rate of small lesions in liver cancer patients. The addition of RD strategy improved the segmentation indicators of liver cancer lesions of the DFN and can fuse image features of multiple sequences, thereby improving the accuracy of lesion segmentation.

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Preoperative transarterial chemoembolization for laparoscopic liver resection in Child A cirrhotic patients with hepatocellular carcinoma

Cited by 8 publications

References 29 publications

New and effective method to develop primary hepatocytes from liver cancer patients

New and effective method to develop primary hepatocytes from liver cancer patients

Effect of Transarterial Chemoembolization Plus Percutaneous Ethanol Injection or Radiofrequency Ablation for Liver Tumors

Automatic Segmentation Algorithm of Magnetic Resonance Image in Diagnosis of Liver Cancer Patients under Deep Convolutional Neural Network

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