Preparation and anti-cancer activity of transferrin/folic acid double-targeted graphene oxide drug delivery system

Lu, Tai-Cheng; Nong, Zhenzhen; Wei, Liying; Wei, Mei; Guo, Li; Wu, Nini; Liu, Cheng; Tang, Bingling; Qin, Qixiao; Li, Xuehua; Meng, Fa‐Yan

doi:10.1177/0885328220913976

Cited by 35 publications

(14 citation statements)

References 51 publications

(60 reference statements)

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“…In FTIR analysis of GO, the adsorption peaks around at 3400, 1720, 1620, and 1100 cm −1 are related to hydroxyl (-OH), carboxyl (C=O), aromatic (C=C) and epoxy (C–O–C) bonds, respectively (Lu et al., 2020 ). After the carboxylation of GO, the resulting derivative (CGO) showed stronger absorption band at 1620 cm −1 , a decrease in hydroxyl bond around at 3400 cm −1 , and an increase in hydroxyl bond around at 2800–3200 cm −1 which confirmed the formation of extra carboxyl groups on the surface of GO.…”

Section: Resultsmentioning

confidence: 99%

Enhanced antitumor activity of bovine lactoferrin through immobilization onto functionalized nano graphene oxide: an in vitro / in vivo study

et al. 2020

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This study aims to improve the anticancer activity of bovine lactoferrin through enhancing its stability by immobilization onto graphene oxide. Bovine lactoferrin was conjugated onto graphene oxide and the conjugation process was confirmed by FT-IR, SDS-PAGE, and UV spectrophotometry. Physical characterization was performed by DLS analysis and atomic force microscopy. The cytotoxicity and cellular uptake of the final construct (CGO-PEG-bLF) was inspected on lung cancer TC-1 cells by MTT assay and flow cytometry/confocal microscopy. The anticancer mechanism of the CGO-PEG-bLF was studied by cell cycle analysis, apoptosis assay, and western blot technique. Finally, the anticancer activity of CGO-PEG-bLF was assessed in an animal model of lung cancer. Size and zeta potential of CGO-PEG-bLF was obtained in the optimum range. Compared with free bLF, more cytotoxic activity, cellular uptake and more survival time was obtained for CGO-PEG-bLF. CGO-PEG-bLF significantly inhibited tumor growth in the animal model. Cell cycle arrest and apoptosis were more induced by CGO-PEG-bLF. Moreover, exposure to CGO-PEG-bLF decreased the phospho-AKT and pro-Caspase 3 levels and increased the amount of cleaved caspase 3 in the treated cells. This study revealed the potential of CGO-PEG as a promising nanocarrier for enhancing the therapeutic efficacy of anticancer agents.

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Section: Resultsmentioning

confidence: 99%

Enhanced antitumor activity of bovine lactoferrin through immobilization onto functionalized nano graphene oxide: an in vitro / in vivo study

et al. 2020

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“…Their high stability and non-toxicity nanocarrier construction depict desirable drug loading and drug releasing functions. They also observed a higher cytotoxicity of loaded DOX at high concentrations rather than free DOX [31].…”

Section: Targeted Drug Delivery Against Broblast Cellsmentioning

confidence: 91%

Loading Harmine On Nanographene Changes The Inhibitory Effects of Free Harmine Against MCF7 and Fibroblast Cells

Mortazavi

Heidari

Rabiei

et al. 2021

Preprint

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Today cancer is one of the main causes of death all over the world. Chemotherapy, which is one of the main therapies in the treatment of cancer, causes several side effects by damaging healthy cells. Therefore, carbon nanomaterial systems have been developed to optimize therapeutics procedures with the least negative consequences. Targeting nanographene oxide (NGO) with folic acid (FA) molecules allows the recognition of MCF-7 cells, which are folic acid receptor (FR) positive. Harmine is a pharmacologically active secondary metabolite that is produced by Peganum harmala. It is found that this metabolite induces apoptosis to human breast cancer cell lines by intercalating DNA molecules. In this study, harmine was loaded on FA-NGO (FA-NGO/harmine) via π-π stacking and hydrophobic interactions and the cytotoxicity against MCF-7, as FR positive cancerous cell, and fibroblast cells, as normal FR negative cell, were investigated. The in vitro studies illustrated that FA-NGO/harmine have remarkably higher cytotoxicity against MCF-7 cells, about 60% cell loss, in comparison with free harmine with 40% cell loss (in the concentration of 40 μg. mL-1). However, the released amount of harmine into normal fibroblast cells was considerably low, only 28% cell loss in dose of 40 μg. mL-1. Our results suggest that the controlled release of harmine into FR positive cancerous cells might have a substantially high cytotoxicity effect.

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“…A novel gene vector, pegylated folate-modified GO/polyethylenimine (PEI) nanocomplexes with a mean size of 216.1 ± 2.457 nm, was found to rapidly escape from the lysosome and release the gene with entrapped siRNA gene, resulting in effective inhibition of the growth of ovarian cancer cells and such a nanoformulation can be used for treatment of folate receptor-positive ovarian tumors [207]. DOX-loaded GO, which was modified by Pluronic ® F68, FA and transferrin, allowing dual-targeting, showed controllable drug delivery performance with no toxicity, exhibited a higher inhibitory efficiency against human hepatocellular carcinoma SMMC-7721 cells than a single target drug delivery system without transferrin functionalization, and showed sustained release, being able to decrease the drug release rate in blood circulation over time and enhance drug concentration in or near a targeted tumor [208]. Nanohybrid prepared by functionalization of GO with Pluronic ® F127 molecules via non-covalent interaction practically did not show any toxicity against human astrocytes and human glioma (U251) cells.…”

Section: Functionalized Gomentioning

confidence: 99%

Advances in Drug Delivery Nanosystems Using Graphene-Based Materials and Carbon Nanotubes

2021

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Carbon is one of the most abundant elements on Earth. In addition to the well-known crystallographic modifications such as graphite and diamond, other allotropic carbon modifications such as graphene-based nanomaterials and carbon nanotubes have recently come to the fore. These carbon nanomaterials can be designed to help deliver or target drugs more efficiently and to innovate therapeutic approaches, especially for cancer treatment, but also for the development of new diagnostic agents for malignancies and are expected to help combine molecular imaging for diagnosis with therapies. This paper summarizes the latest designed drug delivery nanosystems based on graphene, graphene quantum dots, graphene oxide, reduced graphene oxide and carbon nanotubes, mainly for anticancer therapy.

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Preparation and anti-cancer activity of transferrin/folic acid double-targeted graphene oxide drug delivery system

Cited by 35 publications

References 51 publications

Enhanced antitumor activity of bovine lactoferrin through immobilization onto functionalized nano graphene oxide: an in vitro / in vivo study

Enhanced antitumor activity of bovine lactoferrin through immobilization onto functionalized nano graphene oxide: an in vitro / in vivo study

Loading Harmine On Nanographene Changes The Inhibitory Effects of Free Harmine Against MCF7 and Fibroblast Cells

Advances in Drug Delivery Nanosystems Using Graphene-Based Materials and Carbon Nanotubes

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