Preparation and Biological Activity of Molecular Probes to Identify and Analyze Jasmonic Acid-binding Proteins

Transmembrane transport of plant hormones is required for plant growth and development. Despite reports of a number of proteins that can transport the plant hormone gibberellin (GA), the mechanistic basis for GA transport and the identities of the transporters involved remain incomplete. Here, we provide evidence that Arabidopsis SWEET proteins, AtSWEET13 and AtSWEET14, which are members of a family that had previously been linked to sugar transport, are able to mediate cellular GA uptake when expressed in yeast and oocytes. A double sweet13 sweet14 mutant has a defect in anther dehiscence and this phenotype can be reversed by exogenous GA treatment. In addition, sweet13 sweet14 exhibits altered long distant transport of exogenously applied GA and altered responses to GA during germination and seedling stages. These results suggest that AtSWEET13 and AtSWEET14 may be involved in modulating GA response in Arabidopsis.

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“…JA, JA-Ile and 13 C 6 -JA-Ile (ref. 50) were gifted from Dr. Yusuke Jikumaru (RIKEN). D 2 -JA was purchased from Tokyo Kasei.…”

Section: Methodsmentioning

confidence: 99%

AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes

et al. 2016

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“…Stable isotope-labeled compounds used as internal standards were: d2-IAA (Sigma-Aldrich); d6-iP, d5-tZ, d2-GA 1 , d2-GA 4 (Olchemim Ltd, Olomouc, Czech Republic); D6- ABA (Icon Isotopes, Summit, NJ, USA); d6-SA (Sigma-Aldrich); and d2-JA (Tokyo Kasei, Tokyo, Japan). 13C6-JA-Ile was synthesized as described in a previous study [34].…”

Section: Methodsmentioning

confidence: 99%

Phytohormones Related to Host Plant Manipulation by a Gall-Inducing Leafhopper

et al. 2013

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The maize orange leafhopper Cicadulina bipunctata (Hemiptera: Cicadellidae) induces galls characterized by growth stunting and severe swelling of leaf veins on various plants of Poaceae. Previous studies revealed that galls are induced not on feeding site but on distant, newly extended leaves during the feeding, and strongly suggested that some chemicals injected by the leafhopper affect at the leaf primordia. To approach the mechanism underlying gall induction by C. bipunctata, we examined physiological response of plants to feeding by the leafhopper. We performed high-throughput and comprehensive plant hormone analyses using LC-ESI-MS/MS. Galled maize leaves contained higher contents of abscisic acid (ABA) and trans-Zeatin (tZ) and lower contents of gibberellins (GA1 and GA4) than ungalled maize leaves. Leafhopper treatment significantly increased ABA and tZ contents and decreased GA1 and GA4 contents in extending leaves. After the removal of leafhoppers, contents of tZ and gibberellins in extending leaves soon became similar to the control values. ABA content was gradually decreased after the removal of leafhoppers. Such hormonal changes were not observed in leafhopper treatment on leaves of resistant maize variety. Water contents of galled leaves were significantly lower than control leaves, suggesting water stress of galled leaves and possible reason of the increase in ABA content. These results imply that ABA, tZ, and gibberellins are related to gall induction by the leafhopper on susceptible variety of maize.

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“…Kasei, and synthesized 13 C 6 -JA-Ile (Jikumaru et al, 2004) were used as internal standards. Aerial parts of Arabidopsis grown for 4 weeks were frozen with liquid nitrogen, ground with 10-mm ceramic beads, and extracted with 5 volumes of 80% (v/v) methanol containing 1% (v/v) acetic acid and internal standards for 1 h. Extracts were centrifuged at 48C, 14,000g, 10 min, and the supernatant was collected.…”

Section: Quantification Of Phytohormonesmentioning

confidence: 99%

Autophagy Negatively Regulates Cell Death by Controlling NPR1-Dependent Salicylic Acid Signaling during Senescence and the Innate Immune Response inArabidopsis

et al. 2009

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Autophagy is an evolutionarily conserved intracellular process for vacuolar degradation of cytoplasmic components. In higher plants, autophagy defects result in early senescence and excessive immunity-related programmed cell death (PCD) irrespective of nutrient conditions; however, the mechanisms by which cells die in the absence of autophagy have been unclear. Here, we demonstrate a conserved requirement for salicylic acid (SA) signaling for these phenomena in autophagydefective mutants (atg mutants). The atg mutant phenotypes of accelerated PCD in senescence and immunity are SA signaling dependent but do not require intact jasmonic acid or ethylene signaling pathways. Application of an SA agonist induces the senescence/cell death phenotype in SA-deficient atg mutants but not in atg npr1 plants, suggesting that the cell death phenotypes in the atg mutants are dependent on the SA signal transducer NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1. We also show that autophagy is induced by the SA agonist. These findings imply that plant autophagy operates a novel negative feedback loop modulating SA signaling to negatively regulate senescence and immunity-related PCD.

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Preparation and Biological Activity of Molecular Probes to Identify and Analyze Jasmonic Acid-binding Proteins

Cited by 33 publications

References 11 publications

AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes

AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes

Phytohormones Related to Host Plant Manipulation by a Gall-Inducing Leafhopper

Autophagy Negatively Regulates Cell Death by Controlling NPR1-Dependent Salicylic Acid Signaling during Senescence and the Innate Immune Response inArabidopsis

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