Preparation and biological evaluation of [67Ga]-labeled-superparamagnetic nanoparticles in normal rats

Abstract

Gallium-67 / Superparamagnetic iron oxide nanoparticles / Radiolabeling / Stability / BiodistributionSummary. Gallium-67 labeled superparamagnetic iron oxide nanoparticles ([ 67 Ga]-SPION were prepared and evaluated for their altered biodistribution in normal rats. Superparamagnetic iron oxide nanoparticles (SPION) with narrow size distribution were synthesized by a co-precipitation technique using ferrous salts at Fe 3+ /Fe 2+ = 2 molar ratio followed by structure identification using XRD, TGA, DSC, VSM, HRSEM, TEM and FT-IR techniques (≈ 5 nm diameter). In order to trace SPION bio-distribution, the radiolabeled iron oxide nanoparticles were prepared using 67 Ga with a high labeling efficiency (over 96%, RTLC method) and they also showed an excellent stability at room temperature for at least 4 d. The biodistribution of the radiolabeled SPION was checked in normal male rats up to 24 h compared to free Ga 3+ cation biodistribution. The data strongly support the alteration of the tracer accumulation in reticuluendothelial system while the stability of the complex is highly retained.

Recent advances in surface engineering of superparamagnetic iron oxide nanoparticles for biomedical applications

“…More specifically, in the human clinical usage, the SPIONs are being utilized as delivery systems for drugs [25], genes [26], bio-molecules [27] *Corresponding author. E-mail: simchi@sharif.edu and radio-drugs [28], stem cell tracking [29], diabetes [30,31] and cancer detection [32]. There are also numerous in vitro applications for SPIONs in medical diagnostics, such as immune magnetic separation of cells, proteins, DNA/RNA, bacteria, viruses and other biomolecules [33][34][35].…”

Influence of two structural phases of Fe3O4and γ-Fe2O3on the properties of polyimide/iron oxide composites

“…Some studies indicate the specific saturation magnetization of particles corresponding to bulk magnetite and maghemite as 92 and 76 emu g −1 , respectively . Compared with the literature data, the starting magnetite used in present study has a lower saturation magnetization ( M s = 81 emu g −1 , Table ) than the bulk value, generated by the small‐size effect.…”

“…37 Some studies indicate the specific saturation magnetization of particles corresponding to bulk magnetite and maghemite as 92 and 76 emu g −1 , respectively. 12 Compared with the literature data, the starting magnetite used in present study has a lower saturation magnetization (M s = 81 emu g −1 , Table 2) than the bulk value, generated by the small-size effect. The sharp decrease of the saturation magnetization from an initial value of 81 emu g −1 to lower values (between 2.6 and 14.4 emu g −1 ) is probably due to the dielectric matrix, which hinders the strong magnetic interactions.…”

“…Besides its applications in the electronics industry, magnetite could be used in biomedicine as a therapeutic agent for drug delivery, 8 in hyperthermia cancer treatments, 9,10 tumour embolization 11 or in magnetic-field-assisted radionuclide therapy. 12 Therefore, the use of -Fe 2 O 3 and Fe 3 O 4 for biomedical applications requires some properties such as high magnetization values, a narrow particle size distribution and high chemical stability in the environment. 13 In addition, maghemite can be applied in magnetic recording media and ferrofluids.…”

Superparamagnetic Iron Oxide Nanoparticles: Promises for Diagnosis and Treatment of Multiple Sclerosis

“…Very recently, SPIONs have been labeled with radiotracers (183,195), hence offering a great opportunity to probe MS with multimodal equipment (e.g., combination of MRI, PET, and SPECT) (85).…”