Preparation and Candida rugosa Lipase Immobilization on Nylon-6 Grafted and Aminated (Polyvinyl Benzyl Chloride) Microfibers

Halin, Nur Iilani Abd; Al-Khatib, Ma An Fahmi Rashid; Salleh, Hamzah Mohd.; Nasef, Mohamed Mahmoud

doi:10.9767/bcrec.14.2.2894.369-379

Cited by 4 publications

(1 citation statement)

References 26 publications

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“…This trend was seen only when the lipase activity was measured with the use of p-NPB as a substrate. According to literature data, the immobilization times of CRL were 1-5 h (the optimal incubation time was 2 h) [53], 12 h [54], 5 h [55], 1-6 h (the optimal incubation time was 3.5 h) [56]. Regarding the effect of temperature, it is believed that the increased temperature enhances the accessibility and mobility of enzymes.…”

Section: Effect Of Immobilization Conditions On Crl-of Enzymatic Acti...mentioning

confidence: 99%

The Studies of Sepharose-Immobilized Lipases: Combining Techniques for the Enhancement of Activity and Thermal Stability

et al. 2023

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Thermal stability is one of the essential parameters characterizing biocatalysts with potential applications in the chemical and pharmaceutical industries. Therefore, it is extremely important to develop standardized procedures for enzyme stability studies. The paper attempts to assess the thermal stability of immobilized lipases in aqueous buffers: lipase B from Candida antarctica (CALB) and lipase from Candida rugosa (CRL-OF) immobilized on the Octyl-Sepharose CL-4B carrier. As part of the optimization conditions of the immobilization, the influence of time on the catalytic activity and lipase loading, as well as the effect of temperature on lipase activity (optimal incubation—14 h at 4 °C), was determined. The thermal stability test procedure was carried out for 7 days using a climatic chamber (65 °C) and a refrigerator (4 °C). The studies of immobilized lipases included the assessment of the impact of various solvents (water, citrate buffer, 1,2-dichloropropane—DCP), temperature, light in the visible spectral range (400–800 nm), and additions of calcium ions. The highest value of residual activity (564.5 ± 21.6%) was received by storing the immobilized CALB in citrate buffer (pH 4.0, 500 mM) with the addition of calcium ions (Ca2+). On the other hand, residual activity values for immobilized CRL-OF after storage in the climatic chamber were lower than 5%. A combining of techniques: immobilization onto the support in high ionic strength and low pH, with a technique of extremally high-temperature applied in a climatic chamber, with the addition of Ca2+ allowed to achieve of excellent thermal stability of the immobilized CALB, with increasing of catalytic activity more than five-fold. Additionally, performing studies on the thermal stability of the tested lipases using a climatic chamber seems to be particularly promising in the context of unifying and standardizing storage guidelines, enabling the comparison of results between different laboratories, as well as enhancing catalytic activity.

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Section: Effect Of Immobilization Conditions On Crl-of Enzymatic Acti...mentioning

confidence: 99%

The Studies of Sepharose-Immobilized Lipases: Combining Techniques for the Enhancement of Activity and Thermal Stability

et al. 2023

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Photografting Coating: An Innovative Approach to “Non‐Migratory” Active Packaging

Sadeghi

Seo

2021

Adv Funct Materials

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The increasing trend of sustainability (single‐use packaging), safety (fresh consumption), and convenience (processing and application) in packaging science has created a high demand for packaging materials with advanced functions. Thus, the challenges involved with conventional active packaging, for example, migration, low efficiency, upscaling difficulty, safety, and regulations should be addressed through an industrial scalable method, such as photografting coating. Photografting coating, which involves a strong surface covalent linkage, can be employed for preparing novel “non‐migratory” active packaging systems with strong antimicrobial and self‐cleaning, antifouling and self‐defensive, metal chelating as antioxidant, free‐radical scavenging as antioxidant, biocatalytic, and easy printing properties. Herein, profound insights into the technique of photografting coating are provided, with a focus on its application potential in non‐migratory active packaging. The scientific explanation for the photografting coating technique and its application feasibility are illustrated to introduce its potential to confer new functions onto packaging materials. Furthermore, the recent progress, application functions, process, and safety challenges, as well as future directions of photografting coating in the preparation of non‐migratory active packaging systems are explored in detail.

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Candida rugosa lipase immobilized on hydrophobic support Accurel MP 1000 in the synthesis of emollient esters

et al. 2021

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Preparation and Candida rugosa Lipase Immobilization on Nylon-6 Grafted and Aminated (Polyvinyl Benzyl Chloride) Microfibers

Cited by 4 publications

References 26 publications

The Studies of Sepharose-Immobilized Lipases: Combining Techniques for the Enhancement of Activity and Thermal Stability

The Studies of Sepharose-Immobilized Lipases: Combining Techniques for the Enhancement of Activity and Thermal Stability

Photografting Coating: An Innovative Approach to “Non‐Migratory” Active Packaging

Candida rugosa lipase immobilized on hydrophobic support Accurel MP 1000 in the synthesis of emollient esters

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