Preparation and characterisation of composites based on biodegradable polymers for “in vivo” application

Calandrelli, Luigi; Immirzi, Barbara; Malinconico, Mario; Volpe, Maria Grazia; Oliva, Adriana; Ragione, Fulvio Della

doi:10.1016/s0032-3861(00)00165-8

Cited by 63 publications

(58 citation statements)

References 12 publications

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“…The mechanical properties obtained by this technique were about one-third that of trabecular bone. In a comparative study, PCL and biological apatite were mixed in the ratio 19:1 in an extruder [361]. At the end of the preparation, the mixture was cooled in an atmosphere of nitrogen.…”

Section: Apatite-based Biocompositesmentioning

confidence: 99%

“…Besides, an increase in apatite concentration was found to increase both the modulus and yield stress of the composite, which indicated to good interfacial interactions between the biological apatite and PCL. It was also observed that the presence of biological apatite stimulated osteoblasts attachment to the biomaterial and cell proliferation [361]. In another study, a PCL/HA biocomposite was prepared by blending in melt form at 120°C until the torque reached equilibrium in the rheometer that was attached to the blender [362].…”

Section: Apatite-based Biocompositesmentioning

confidence: 99%

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Calcium orthophosphate-based biocomposites and hybrid biomaterials

Dorozhkin¹

2009

J Mater Sci

285

146

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Section: Apatite-based Biocompositesmentioning

confidence: 99%

Section: Apatite-based Biocompositesmentioning

confidence: 99%

Calcium orthophosphate-based biocomposites and hybrid biomaterials

Dorozhkin¹

2009

J Mater Sci

285

146

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“…PLGA and PLLA have been combined with HAP and β-TCP to form biodegradable polymer/ceramic composites with the goal of increasing osteoconductivity, buffering acidic byproducts and increasing the compressive modulus of the pure polymeric materials [76][77][78]. One such porous scaffold comprised of PLGA and β-TCP, seeded with mesenchymal stem cells, was cultivated in osteogenic medium over a 40 day period resulting in increased collagen type I and osteocalcin in the scaffold as compared…”

Section: Compositesmentioning

confidence: 99%

“…This study confirmed that cell attachment, proliferation and ECM production was increased with addition of β-TCP to the PLGA scaffold. In a similar study, PLLA was combined with ossein, a biological HAP, seeded with osteoblast precursor cells, and was tested to determine thermal, morphological, mechanical and osteoconductivity properties of the composites [77]. Compressive modulus of the composite scaffold was greater than the PLLA scaffold and a greater osteoblast phenotypic response was seen with the composite as compared to the PLLA scaffold [77].…”

Section: Compositesmentioning

confidence: 99%

Osteoblast response to zirconia‐hybridized pyrophosphate‐stabilized amorphous calcium phosphate

Whited

Škrtić

Love

et al. 2005

J Biomedical Materials Res

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(Abstract)Biodegradable polyesters, such as poly(DL-lactic-co-glycolic acid) (PLGA), have been used to fabricate porous bone scaffolds to support bone tissue development. These scaffolds allow for cell seeding, attachment, growth and extracellular matrix production in vitro and are replaced by new bone tissue when implanted into bone sites in vivo.Hydroxyapatite (HAP) and β-tricalcium phosphate (β-TCP) ceramics have been incorporated into PLGA bone scaffolds and have been shown to increase their osteoconductivity (support cell attachment). Although HAP, β-TCP, and biodegradable polyesters are osteoconductive, there is no evidence that these scaffold materials are osteoinductive (support cell differentiation). Calcium and phosphate ions, in contrast, have been postulated to be osteogenic factors that enhance osteoblast differentiation and mineralization. Recently, a zirconia-hybridized pyrophosphate stabilized amorphous calcium phosphate (Zr-ACP) has been synthesized which permits controlled release of calcium and phosphate ions and thus is hypothesized to be osteoinductive. Incorporation of Zr-ACP into a highly porous poly(DL lactic-co-glycolic acid) (PLGA) scaffold could potentially increase the osteoinductivity of the scaffold and therefore promote osteogenesis when implanted in vivo.To determine the osteoinductivity of Zr-ACP, a MC3T3-E1 mouse calvarialderived osteoprogenitor cell line was used to measure cell response to Zr-ACP. To accomplish this objective, Zr-ACP was added to cell culture at different stages in cell maturation (days 0, 4 and 11). DNA synthesis, alkaline phosphatase (ALP) activity, iii osteopontin synthesis and collagen synthesis were determined. Results indicate that culture in the presence of Zr-ACP significantly increased cell proliferation, ALP activity and osteopontin synthesis but not collagen synthesis. To determine the feasibility of incorporating Zr-ACP into a PLGA scaffold, PLGA/Zr-ACP composite foams (5% or 10% (w/v) polymer:solvent with 25 wt% or 50 wt% Zr-ACP) were fabricated using a thermal phase inversion technique. Scanning electron microscopy revealed a highly porous structure with pores ranging in size from a few microns to about 100 µm. The amorphous structure of the Zr-ACP was maintained during composite fabrication as confirmed by X-ray diffraction measurements. Composite scaffolds also showed significantly greater compressive yield strengths and moduli as compared to pure polymer scaffolds.The results of this study indicate that Zr-ACP enhances the osteoblastic phenotype of MC3T3-E1 cells in vitro and can be incorporated into a porous PLGA scaffold. Porous PLGA/Zr-ACP composites are promising for use as bone scaffolds to heal bone defects.iv

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“…These biopolymers also have low to xicity reactions with the body and their degradation rate can be easily controlled. Examp les of synthetic biodegradable polymers include Poly (lact ic acid), PLA [57][58][59][60][61][62], Po ly (L-lactic acid), PLLA [63][64][65][66], Po ly (lactic-co-glycolic acid), PLGA [67][68][69][70], Poly-capro lactone PCL [71][72][73][74] and Poly (g lycolic acid ), PGA [75][76][77][78]. These biopolymers are generally poly-α-hydro x esters that de-esterifies in the body as the polymer degrades to simp le metabolites [79].…”

Section: Introductionmentioning

confidence: 99%

Biomedical Magnesium Alloys: A Review of Material Properties, Surface Modifications and Potential as a Biodegradable Orthopaedic Implant

Poinern¹,

Brundavanam²,

Fawcett³

2013

AJBE

283

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Magnesium and magnesium based alloys are lightweight metallic materials that are extremely biocompatib le and have similar mechanical properties to natural bone. These materials have the potential to function as an osteoconductive and biodegradable substitute in load bearing applicat ions in the field of hard t issue engineering. However, the effects of corrosion and degradation in the physiological environ ment of the body has prevented their wide spread applicat ion to date. The aim o f this review is to examine the properties, chemical stability, degradation in situ and methods of improving the corrosion resistance of magnesium and its alloys for potential application in the orthopaedic field. To be an effective imp lant, the surface and sub-surface properties of the material needs to be carefully selected so that the degradation kinetics of the implant can be efficiently controlled. Several surface modification techniques are presented and their effectiveness in reducing the corrosion rate and methods of controlling the degradation period are discussed. Ideally, balancing the gradual loss of material and mechanical strength during degradation, with the increasing strength and stability of the newly forming bone tissue is the ultimate goal. If this goal can be achieved, then orthopaedic implants manufactured fro m magnesium based alloys have the potential to deliver successful clinical outcomes without the need for revision surgery.

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Preparation and characterisation of composites based on biodegradable polymers for “in vivo” application

Cited by 63 publications

References 12 publications

Calcium orthophosphate-based biocomposites and hybrid biomaterials

Calcium orthophosphate-based biocomposites and hybrid biomaterials

Osteoblast response to zirconia‐hybridized pyrophosphate‐stabilized amorphous calcium phosphate

Biomedical Magnesium Alloys: A Review of Material Properties, Surface Modifications and Potential as a Biodegradable Orthopaedic Implant

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