Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel

Gong, Xiao; Branford-White, Christopher; Tao, Lei; Li, Shubai; Quan, Jing; Nie, Huali; Zhu, Li‐Min

doi:10.1016/j.msec.2015.08.059

Cited by 47 publications

(34 citation statements)

References 48 publications

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“…The FTIR spectra (Figure 4b) showed the broad peak centered around 1,650 cm −1 which is consistent with a βsheet structure (17,20,23). Because of the dominant role of hydrogen bonds and aromatic interactions, Fmoc-FF peptides tend to self-assemble into structures with βsheet patterns, regardless of the IDM incorporation into the hydrogels, in line with the CD spectra.…”

Section: Properties Of Fmoc-ff-idm Hydrogelssupporting

confidence: 60%

“…The biocompatibility, biodegradability, and nonimmunogenicity of dipeptide-based LMWGs make them attractive candidates for biomedical applications. Within this class, Fmoc-modified diphenylalanine (Fmoc-FF) has been widely studied as the simplest and most effective hydrogelator (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26). Fmoc-FF has been shown to form β-sheets that selfassemble laterally through π-π stacking of the fluorenyl groups into nanofibrous networks in which water is entrapped mainly by surface tension (15).…”

Section: Introductionmentioning

confidence: 99%

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Fmoc-diphenylalanine-based hydrogels as a potential carrier for drug delivery

Choe

Yun

2020

E-Polymers

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Self-assembled hydrogels from 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) peptides were evaluated as potential vehicles for drug delivery. During self-assembly of Fmoc-FF, high concentrations of indomethacin (IDM) drugs were shown to be incorporated into the hydrogels. The β-sheet arrangement of peptides was found to be predominant in Fmoc-FF–IDM hydrogels regardless of the IDM content. The release mechanism for IDM displayed a biphasic profile comprising an initial hydrogel erosion-dominated stage followed by the diffusion-controlled stage. Small amounts of polyamidoamine dendrimer (PAMAM) added to the hydrogel (Fmoc-FF 0.5%–IDM 0.5%–PAMAM 0.03%) resulted in a more prolonged IDM release compared with Fmoc-FF 0.5%–IDM 0.5% hydrogel. Furthermore, these IDM-loaded hydrogels demonstrated excellent thixotropic response and injectability, which make them suitable candidates for use as injectable self-healing matrices for drug delivery.

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Section: Properties Of Fmoc-ff-idm Hydrogelssupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Fmoc-diphenylalanine-based hydrogels as a potential carrier for drug delivery

Choe

Yun

2020

E-Polymers

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“…A notable example of Fmoc-based hydrogels is the Fmoc-diphenylalanine (FmocFF) peptide that efficiently assembles to form a rigid nanofibrous hydrogel under physiological conditions [52,53]. FmocFF has been combined with different polysaccharides to form new composite hydrogels for different biomedical applications, including cell culture [31,54,55] and drug delivery [13,54,56,57].…”

Section: Introductionmentioning

confidence: 99%

Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration

2019

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The high demand for tissue engineering scaffolds capable of inducing bone regeneration using minimally invasive techniques prompts the need for the development of new biomaterials. Herein, we investigate the ability of Alginate incorporated with the fluorenylmethoxycarbonyl-diphenylalanine (FmocFF) peptide composite hydrogel to serve as a potential biomaterial for bone regeneration. We demonstrate that the incorporation of the self-assembling peptide, FmocFF, in sodium alginate leads to the production of a rigid, yet injectable, hydrogel without the addition of cross-linking agents. Scanning electron microscopy reveals a nanofibrous structure which mimics the natural bone extracellular matrix. The formed composite hydrogel exhibits thixotropic behavior and a high storage modulus of approximately 10 kPA, as observed in rheological measurements. The in vitro biocompatibility tests carried out with MC3T3-E1 preosteoblast cells demonstrate good cell viability and adhesion to the hydrogel fibers. This composite scaffold can induce osteogenic differentiation and facilitate calcium mineralization, as shown by Alizarin red staining, alkaline phosphatase activity and RT-PCR analysis. The high biocompatibility, excellent mechanical properties and similarity to the native extracellular matrix suggest the utilization of this hydrogel as a temporary three-dimensional cellular microenvironment promoting bone regeneration.

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“…According to the literature, the enhanced hydrogen bonding into the self-assembled peptide strengthen the stability of hydrogels. 54 The Table 1 presents the coefficients obtained with equation 1 adjusted for the G' results of each hydrogel. The values of n and S allow for a quantitative estimation of the hydrogel's strength, whereas for much higher S and G' with a lower ω, a higher strength is exhibited by the hydrogel, indicative of the increase of entanglements resulting from the solvent used.…”

Section: Resultsmentioning

confidence: 99%

Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection

Souza

Kogikoski

Silva

et al. 2016

J. Braz. Chem. Soc.

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Hydrogels based on peptide nanostructures are biological entities that can be applied in a wide range of applications, such as scaffolds for tissue engineering, drug delivery, and biosensors. The aim of this research was to study peptide hydrogels based on N-(9-fluorenylmethoxycarbonyl)-L,L-diphenylalanine (Fmoc-FF) in two different media: water and phosphate buffer. These hydrogels were used for encapsulating Leishmania infantun chagasi soluble proteins. The structure of the matrices was investigated in detail through scanning and transmission electron microscopy, and small angle X-ray scattering (SAXS). The mechanical behavior of the hydrogels were assessed through rheology assays, demonstrating both the physical and chemical stability of the hydrogel scaffolds. The immunogenicity of immobilized antigens was studied using enzyme-linked immunosorbent assay (ELISA) detection after the reaction with positive and negative dog sera for Leishmania infantum chagasi. The hydrogel was efficient to encapsulate antigens, and can promote the development of novel devices that requires the storage of biomolecules under moist environmental conditions.

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Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel

Cited by 47 publications

References 48 publications

Fmoc-diphenylalanine-based hydrogels as a potential carrier for drug delivery

Fmoc-diphenylalanine-based hydrogels as a potential carrier for drug delivery

Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration

Nanostructured Antigen-Responsive Hydrogels Based on Peptides for Leishmaniasis Detection

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