Preparation and characterization of an injectable dexamethasone-cyclodextrin complexes-loaded gellan gum hydrogel for cartilage tissue engineering

Choi, Joo Hee; Park, Ain; Lee, Wonchan; Youn, Jina; Rim, Min A.; Kim, Wooyoup; Kim, Namyeong; Song, Jeong Eun; Khang, Gilson

doi:10.1016/j.jconrel.2020.08.049

Cited by 43 publications

(17 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The encapsulated cells in both the experimental groups were uniformly dispersed in the porous structure and did not display any abnormal cells. The morphology of the cells was spherical, which is a favorable structure for chondrogenesis [7]. A greater number of cells were observed on 21 days of culture in both groups, which may be due to the proliferation of cells in the matrix.…”

Section: Morphology and Histological Observationmentioning

confidence: 87%

“…The emergence of cartilage tissue engineering has led to a surge in biomaterial demand. The objective in designing biomaterials is to provide a good environment for cell growth and mechanically support regenerated tissue [6,7]. As a kind of biomaterial, hydrogel has been widely studied as an alternative to cartilage tissue due to its inherent water content, high hydrophilicity, and viscoelasticity similar to cartilage tissue [8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

et al. 2021

Self Cite

View full text Add to dashboard Cite

Hydrogel is in the spotlight as a useful biomaterial in the field of drug delivery and tissue engineering due to its similar biological properties to a native extracellular matrix (ECM). Herein, we proposed a ternary hydrogel of gellan gum (GG), silk fibroin (SF), and chondroitin sulfate (CS) as a biomaterial for cartilage tissue engineering. The hydrogels were fabricated with a facile combination of the physical and chemical crosslinking method. The purpose of this study was to find the proper content of SF and GG for the ternary matrix and confirm the applicability of the hydrogel in vitro and in vivo. The chemical and mechanical properties were measured to confirm the suitability of the hydrogel for cartilage tissue engineering. The biocompatibility of the hydrogels was investigated by analyzing the cell morphology, adhesion, proliferation, migration, and growth of articular chondrocytes-laden hydrogels. The results showed that the higher proportion of GG enhanced the mechanical properties of the hydrogel but the groups with over 0.75% of GG exhibited gelling temperatures over 40 °C, which was a harsh condition for cell encapsulation. The 0.3% GG/3.7% SF/CS and 0.5% GG/3.5% SF/CS hydrogels were chosen for the in vitro study. The cells that were encapsulated in the hydrogels did not show any abnormalities and exhibited low cytotoxicity. The biochemical properties and gene expression of the encapsulated cells exhibited positive cell growth and expression of cartilage-specific ECM and genes in the 0.5% GG/3.5% SF/CS hydrogel. Overall, the study of the GG/SF/CS ternary hydrogel with an appropriate content showed that the combination of GG, SF, and CS can synergistically promote articular cartilage defect repair and has considerable potential for application as a biomaterial in cartilage tissue engineering.

show abstract

Section: Morphology and Histological Observationmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the other hand, the morphology of the C28/I2 cells was analyzed after being seeded on the surface of the hydrogels for 72 h. Cell shape plays an important role in the phenotypic expression of chondrocytes. The rounded morphology indicates characteristics of the chondrogenic phenotype [ 57 , 58 ]. Figure 3 B shows the SEM micrographs of the cells seeded in the materials, (a) CS1-PF25 and (b) CS1-PF25-TPP.…”

Section: Resultsmentioning

confidence: 99%

Chitosan/Pluronic F127 Thermosensitive Hydrogel as an Injectable Dexamethasone Delivery Carrier

García-Couce

Tomás²,

Fuentes

et al. 2022

Gels

View full text Add to dashboard Cite

Intra-articular administration of anti-inflammatory drugs is a strategy that allows localized action on damaged articular cartilage and reduces the side effects associated with systemic drug administration. The objective of this work is to prepare injectable thermosensitive hydrogels for the long-term application of dexamethasone. The hydrogels were prepared by mixing chitosan (CS) and Pluronic-F127 (PF) physically. In addition, tripolyphosphate (TPP) was used as a crosslinking agent. Chitosan added to the mix increased the gel time compared to the pluronic gel alone. The incorporation of TPP into the material modified the morphology of the hydrogels formed. Subsequently, MTS and Live/Dead® experiments were performed to investigate the toxicity of hydrogels against human chondrocytes. The in vitro releases of dexamethasone (DMT) from CS-PF and CS-PF-TPP gels had an initial burst and took more time than that from the PF hydrogel. In vivo studies showed that hydrogels retained the fluorescent compound longer in the joint than when administered in PBS alone. These results suggest that the CS-PF and CS-PF-TPP hydrogels loaded with DMT could be a promising drug delivery platform for the treatment of osteoarthritis.

show abstract

“…It was attributed to migration of β‐CD‐HDA, a small building unit, that has the benefit of transporting hydrophobic drugs. [ 55,66 ] This demonstrated that the hydrogel had the potential to be used in a drug delivery system; thus, the drug release behavior and biocompatibility will be investigated further.…”

Section: Resultsmentioning

confidence: 99%

Stimuli‐Responsive, Self‐Healing, and Injectable Hydrogels with Dual‐Crosslinked Design from Phenolphthalein‐Grafted N‐Carboxyethyl Chitosan

Savetsakulanont

Chalitangkoon

Monvisade

2021

Macro Materials & Eng

View full text Add to dashboard Cite

A new biomaterial, a hydrogel, with dual‐crosslinked design, has been created with enhanced mechanical performance. The hydrogels are fabricated based on water‐soluble chitosan, with dual‐crosslinking of imine linkages and host–guest interactions. Phenolphthalein‐grafted N‐carboxyethyl chitosan (CECS‐g‐PHP), as a guest polymer, is synthesized and structurally characterized and complexed with hexamethylenediamine modified β‐cyclodextrin (β‐CD‐HDA), as a host molecule. Oxidized sodium alginate (OSA) is added to form crosslinking networks via imine linkages with the existing amino groups. The hydrogels show significantly shorter gelation times and higher compressive stresses, compared with single‐crosslinked hydrogels. The phenolphthalein units in the hydrogel change color with pH and other added chemicals. Moreover, the hydrogels can be injected and are self‐healing with >80% recovery within 4 h. Thus, these dual‐crosslinked hydrogels, which respond to pH and other stimuli, are promising designs for new multifunctional biomaterials.

show abstract

Preparation and characterization of an injectable dexamethasone-cyclodextrin complexes-loaded gellan gum hydrogel for cartilage tissue engineering

Cited by 43 publications

References 65 publications

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

Chitosan/Pluronic F127 Thermosensitive Hydrogel as an Injectable Dexamethasone Delivery Carrier

Stimuli‐Responsive, Self‐Healing, and Injectable Hydrogels with Dual‐Crosslinked Design from Phenolphthalein‐Grafted N‐Carboxyethyl Chitosan

Contact Info

Product

Resources

About