Preparation and characterization of blended Bombyx mori silk fibroin scaffolds

Tiyaboonchai, Waree; Chomchalao, Pratthana; Pongcharoen, Sutatip; Sutheerawattananonda, Manote; Sobhon, Prasert

doi:10.1007/s12221-011-0324-9

Cited by 27 publications

(16 citation statements)

References 56 publications

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“…Three dimensional scaffolds of silk fibroin (SF), silk fibroin/collagen (SF/C), and silk fibroin/gelatin (SF/G) were prepared according to the procedure described in our previous study using a freeze-drying technique [30]. Briefly, SF solutions were prepared from 6% w/v silk fibroin aqueous solution.…”

Section: Methodsmentioning

confidence: 99%

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Fibroin and fibroin blended three-dimensional scaffolds for rat chondrocyte culture

et al. 2013

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BackgroundIn our previous study, we successfully developed 3-D scaffolds prepared from silk fibroin (SF), silk fibroin/collagen (SF/C) and silk fibroin/gelatin (SF/G) using a freeze drying technique. The blended construct showed superior mechanical properties to silk fibroin construct. In addition, collagen and gelatin, contain RGD sequences that could facilitate cell attachment and proliferation. Therefore, in this study, the ability of silk fibroin and blended constructs to promote cell adhesion, proliferation and production of extracellular matrix (EMC) were compared.MethodsArticular chondrocytes were isolated from rat and cultured on the prepared constructs. Then, the cell viability in SF, SF/C and SF/G scaffolds was determined by MTT assay. Cell morphology and distribution were investigated by scanning electron microscopy (SEM) and histological analysis. Moreover, the secretion of extracellular matrix (ECM) by the chondrocytes in 3-D scaffolds was assessed by immunohistochemistry.ResultsResults from MTT assay indicated that the blended SF/C and SF/G scaffolds provided a more favorable environment for chondrocytes attachment and proliferation than that of SF scaffold. In addition, scanning electron micrographs and histological images illustrated higher cell density and distribution in the SF/C and SF/G scaffolds than that in the SF scaffold. Importantly, immunohistochemistry strongly confirmed a greater production of type II collagen and aggrecan, important markers of chondrocytic phenotype, in SF blended scaffolds than that in the SF scaffold.ConclusionAddition of collagen and gelatin to SF solution not only improved the mechanical properties of the scaffolds but also provided an effective biomaterial constructs for chondrocyte growth and chondrocytic phenotype maintenance. Therefore, SF/C and SF/G showed a great potential as a desirable biomaterial for cartilage tissue engineering.

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Section: Methodsmentioning

confidence: 99%

“…In our previous study, we successfully developed 3-D scaffolds prepared from SF, SF/C and SF/G using a freeze drying technique with methanol treatment [30]. These scaffolds exhibited sponge-like structure with homogeneous interconnecting pores and high water adsorption.…”

Section: Introductionmentioning

confidence: 99%

Fibroin and fibroin blended three-dimensional scaffolds for rat chondrocyte culture

et al. 2013

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“…ACCEPTED MANUSCRIPT 20 to a high frequency region [16,42]. The absorption of amide Ⅱ in chitosan decreased in both RHC-chitosan composite and PA cross-linked scaffold also suggests that the -NH 2 groups were changed into N-H [31].…”

Section: Accepted Manuscriptmentioning

confidence: 98%

HPLC detection of loss rate and cell migration of HUVECs in a proanthocyanidin cross-linked recombinant human collagen-peptide (RHC)–chitosan scaffold

Zhang

Deng

Yang

et al. 2015

Materials Science and Engineering: C

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“…The SF/CS/n H A scafford was prepa red as previously described (10). SF, CS and nHA were prepared into 2% solutions and then combined in three proportions, namely 1:1:0.5, 1:1:1 and 1:1:1.5.…”

Section: Methodsmentioning

confidence: 99%

Application of silk fibroin/chitosan/nano‑hydroxyapatite composite scaffold in the repair of rabbit radial bone defect

Deng

et al. 2017

Exp Ther Med

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Preparation and characterization of blended Bombyx mori silk fibroin scaffolds

Cited by 27 publications

References 56 publications

Fibroin and fibroin blended three-dimensional scaffolds for rat chondrocyte culture

Fibroin and fibroin blended three-dimensional scaffolds for rat chondrocyte culture

HPLC detection of loss rate and cell migration of HUVECs in a proanthocyanidin cross-linked recombinant human collagen-peptide (RHC)–chitosan scaffold

Application of silk fibroin/chitosan/nano‑hydroxyapatite composite scaffold in the repair of rabbit radial bone defect

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