Preparation and Characterization of Chitosan/mPEG-PCL Blended Membranes for Wound Dressing and Controlled Gentamicin Release

Brianezi, Samira Faleiros Silva; Castro, Karine Cappuccio de; Piazza, Rodolfo Debone; Melo, Maria do Socorro Fernandes; Pereira, Rafael Matsumoto; Marques, Rodrigo Fernando Costa; Campos, Maria Gabriela Nogueira

doi:10.1590/1980-5373-mr-2017-0951

Cited by 11 publications

(8 citation statements)

References 23 publications

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“…The PCL fibers' spectra indicate a strong sharp peak around 1717 cm −1 , which is due to C=O vibration and at 2942 cm −1 which represented a C-H peak [50]. Moreover, several characteristic bands of PCL were found at 2949 cm -1 (asymmetric -CH 2 stretching), 2865 cm −1 (symmetric -CH 2 stretching), 1726 cm −1 (carbonyl stretching), 1293 cm −1 (C-O and C-C stretching), 1240 cm −1 (asymmetric C-O-C stretching), and 1170 cm −1 (symmetric C-O-C stretching) [35,36] (Figure S1).…”

Section: Fourier Transform Infrared Spectroscopymentioning

confidence: 98%

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Bacterial Biofilm Formation Using PCL/Curcumin Electrospun Fibers and Its Potential Use for Biotechnological Applications

et al. 2020

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Electrospun nanofibers are used for many applications due to their large surface area, mechanical properties, and bioactivity. Bacterial biofilms are the cause of numerous problems in biomedical devices and in the food industry. On the other hand, these bacterial biofilms can produce interesting metabolites. Hence, the objective of this study is to evaluate the efficiency of poly (Ɛ- caprolactone)/Curcumin (PCL/CUR) nanofibers to promote bacterial biofilm formation. These scaffolds were characterized by scanning electron microscopy (SEM), which showed homogeneous fibers with diameters between 441–557 nm; thermogravimetric analysis and differential scanning calorimetry (TGA and DSC) demonstrated high temperature resilience with degradation temperatures over >350 °C; FTIR and 1H-NMR serve as evidence of CUR incorporation in the PCL fibers. PCL/CUR scaffolds successfully promoted the formation of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa biofilms. These results will be valuable in the study of controlled harvesting of pathogenic biofilms as well as in metabolites production for biotechnological purposes.

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Section: Fourier Transform Infrared Spectroscopymentioning

confidence: 98%

“…PCL and PCL/CUR casting films were prepared using PCL and PCL/CUR solutions obtained in Section 2.2. The membranes were obtained by casting 1.5 mL of each solution in a circular mold (diameter 2.5 cm), followed by the evaporation of solvent at room temperature according to Brianezi et al [35].…”

Section: Preparation Of Pcl/cur Cast Filmsmentioning

confidence: 99%

Bacterial Biofilm Formation Using PCL/Curcumin Electrospun Fibers and Its Potential Use for Biotechnological Applications

et al. 2020

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“…All gentamicin-loaded membranes inhibited S. aureus and E. coli growth, and demonstrated color, moisture and thermal stability. Therefore, mPEG-PCL/CHI-genipin membranes showed important features for potential wound dressing and drug delivery applications [132] . An interesting tissue-engineered skin substitute, based on gelatin, collagen, and PCL, was fabricated by impregnation of lyophilized gelatin/collagen (GC) mats with PCL solutions, followed by solvent evaporation.…”

Section: Biomaterials For Wound Managementmentioning

confidence: 99%

Recent trends on wound management: New therapeutic choices based on polymeric carriers

Okur

Karantas

Şenyiğit

et al. 2020

Asian Journal of Pharmaceutical Sciences

224

115

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Wound healing is an unmet therapeutic challenge among medical society since wound assessment and management is a complex procedure including several factors playing major role in healing process. Wounds can mainly be categorized as acute or chronic. It is well referred that the acute wound displays normal wound physiology while healing, in most cases, is seemed to progress through the normal phases of wound healing. On the other hand, a chronic wound is physiologically impaired. The main problem in wound management is that the majority of wounds are colonized with microbes, whereas this does not mean that all wounds will be infected. In this review, we address the problems that clinicians face to manage while treat acute and chronic wounds. Moreover, we demonstrate the pathophysiology, etiology, prognosis and microbiology of wounds. We further introduce the state of art in pharmaceutical technology field as part of wound management aiming to assist health professionals to overcome the current implications on wound assessment. In addition, authors review researches which included the use of gels and dermal films as wound healing agents. It can be said that natural and synthetic drugs or carriers provide promising solutions in order to meet the wound management standards. However, are the current strategies as desirable as medical society wish?

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“…Another alternative to improve the miscibility of gentamicin with PVA and chitosan is by chemically modifying the polymer backbone or adding molecules that consists of both hydrophobic and hydrophilic moieties (i.e. surfactant) [31]. The diameter of the nanofibers and the drug's physical properties such as solubility and drug-polymer interactions may significantly affect the drug release rate [28].…”

Section: In Vitro Drug Release Studymentioning

confidence: 99%

In Vitro Evaluation of Crosslinked Polyvinyl Alcohol/Chitosan – Gentamicin Sulfate Electrospun Nanofibers

Hamdan¹,

Susanti²,

Khodir³

2021

MJCHEM

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Polymeric nanofibers with good antimicrobial properties are a promising option to thwart wound infection and accelerate wound healing. Although PVA/Chitosan possesses many useful properties, its antibacterial activity is insufficient for effective wound dressing. Therefore, using a hydrophilic drug such as Gentamicin Sulfate (GS) that has a broad-spectrum activity against a wide range of bacteria can enhance the nanofibers' performance. In this study, polyvinyl alcohol (PVA) with chitosan nanofibers loaded with gentamicin sulfate was prepared using an electrospinning technique and crosslinked with glutaraldehyde for better loading efficiency and controlled drug release at the site of interest. Morphological investigation carried out using scanning electron microscopy showed smooth and homogeneous nanofibers. FT-IR was used to confirm the structure of the nanofibers. In situ crosslinking enabled penetration of the crosslinking agent into the nanofibers and improved the thermal stability and drug release performance. The thermal stability of PVA/Chitosan nanofibers was reduced with the addition of gentamicin sulfate. The kinetic release of gentamicin sulfate followed the Korsmeyer-Peppas model with release exponent, n < 0.5. Antibacterial testing of crosslinked nanofibers against Escherichia coli and Staphylococcus aureus showed good inhibition of bacterial growth. Crosslinked PVA/Chitosan nanofibers loaded with gentamicin sulfate showed multifunctional characteristics and thus may be a suitable material for controlled drug delivery and tissue engineering applications.

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Preparation and Characterization of Chitosan/mPEG-PCL Blended Membranes for Wound Dressing and Controlled Gentamicin Release

Cited by 11 publications

References 23 publications

Bacterial Biofilm Formation Using PCL/Curcumin Electrospun Fibers and Its Potential Use for Biotechnological Applications

Bacterial Biofilm Formation Using PCL/Curcumin Electrospun Fibers and Its Potential Use for Biotechnological Applications

Recent trends on wound management: New therapeutic choices based on polymeric carriers

In Vitro Evaluation of Crosslinked Polyvinyl Alcohol/Chitosan – Gentamicin Sulfate Electrospun Nanofibers

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