Preparation and Characterization of Coaxial Electrospinning rhBMP2-Loaded Nanofiber Membranes

Abstract

DEX and rhBMP2-loaded core-shell nanofiber membranes were synthesized by electrospinning method in one step. Zein/PLLA, Zein-DEX/PLLA, Zein/PLLA-rhBMP2, and Zein-DEX/PLLA-rhBMP2 were fabricated; and morphology, hydrophilicity, mechanics properties, in vitro drug release behavior, cell proliferation, and osteogenic differentiation were investigated. The results showed that the dual-release system containing rhBMP2 and DEX prepared by electrospinning had rough surface, constant drug release behavior, and could also significantly promote cell proliferation and osteogenic differentiation of RMSCs, indicating that the scaffolds we fabricated might be suitable for bone tissue engineering.

A Review of Zein as a Potential Biopolymer for Tissue Engineering and Nanotechnological Applications

“…The recombinant human bone morphogenetic protein 2 (rhBMP-2) and dexamethasone (DEX) enhance the TE differentiation efficacy. Herein, it is necessary to maintain the release of the stable factor [80].…”

Agro-Industrial Plant Proteins in Electrospun Materials for Biomedical Application

“…The recombinant human bone morphogenetic protein 2 and dexamethasone enhance tissue differentiation efficacy. Herein, it is necessary to maintain the stable release of the factor [ 54 ]. In a technically more complex approach, researchers designed a bilayer membrane with a unique drug delivery property combined with coaxial electrospinning and 3D printing aimed at periodontal tissue regeneration [ 55 ].…”

Optimized Loading of TiO2 Nanoparticles into Electrospun Polyacrylonitrile and Cellulose Acetate Polymer Fibers

“…The morphology and diameter of the electrospun nanofibers depend on the solution parameters such as viscosity, molecular weight, surface tension, and conductivity [17]. Electrospinning is a fast developing technique with many facets ranging from a single-fluid process [18] to other modified coaxial processes [19][20][21]. Electrospinning provides opportunity to create novel nanomaterials and endow fibers new functional properties.…”

“…A sol-gel method has been an attractive method for the synthesis of nanoparticles among other methods due to its simplicity, low cost, and the production of desirable structural characteristics of the nanoparticles [19]. Most products obtained via the sol-gel method are amorphous; hence, posttreatment such as the calcination method is preferred to modify the crystal phase, particle size, specific surface area, and morphology of TiO 2 nanoparticles for different applications [25,26].…”