Preparation and characterization of electrospun alginate nanofibers loaded with ciprofloxacin hydrochloride

Abstract

Alginate nanofibers were prepared in the presence of poly(ethylene oxide) (PEO), a surfactant Pluronic F-127, and a model drug (ciprofloxacin hydrochloride, CpHCl), all mixed prior to electrospinning. It was demonstrated that addition of a carrier polymer (PEO) and a small amount of surfactant are necessary to obtain uniform alginate fibers with cylindrical shape and regular morphology. Importantly, PEO was completely removed from the resulting nanofibers during crosslinking and stabilization post-treatment. The stable alginate fibers loaded with CpHCl were examined by scanning electron microscopy and the average diameter of the fibers ranged from 109 nm (unloaded fibers) to 161 nm (loaded fibers). The release of a studied antibiotic from the nanofibers, characterized by a final loading efficiency of 51%, was tested in physiological conditions. It was revealed that ca. 24% of CpHCl is released during first 20h with combined transport mechanism, however with the predominant contribution of Fickian diffusion.

Natural Antibacterial Reagents (Centella, Propolis, and Hinokitiol) Loaded into Poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] Composite Nanofibers for Biomedical Applications

“…This decline might be due to the degradation of hinokitiol in PBS solution by light, heat, or solvent (ethanol). In the study of ciprofloxacin hydrochloride (CpHCl) release from electrospun alginate [42], at around 24% release of total loaded drug, the cross-linking process between CpHCl molecules in the nanofibers happened and affected the release behaviors. This result also suggests the relation between the release capacity and the characteristics of PHBH, which is hydrophobic polymer.…”

Electrospun Alginate Nanofibers Toward Various Applications: A Review

“…The replacement of the co-solvent and surfactant can be achieved by modification of the alginate in order to replace the -OH groups, either via esterification, oxidation, or sulfation [7,21,[42][43][44][45][46]. These processes introduce other functional groups onto the backbone of alginate by replacing the -OH groups, thereby reducing the intra-and inter-molecular hydrogen bonding network, which contributes to the rigidity in chain conformation [18,42].…”

Advances in Electrospinning of Natural Biomaterials for Wound Dressing

“…The obtained macroscopic results Journal of Nanomaterials and histopathological analysis showed that both the colloidal solution and the microfibers had shown statistically equivalent results as the respective commercial products, although the amount of silver applied to the wound was about two orders of magnitude lower. Kyzioł et al [89] prepared alginate nanofibers in the presence of poly(ethylene oxide) (PEO), a surfactant Pluronic F-127, and a model drug (ciprofloxacin hydrochloride, CpHCl), all mixed prior to electrospin. It was demonstrated that the addition of a carrier polymer (PEO) and a small amount of surfactant are necessary to obtain uniform alginate fibers with cylindrical shape and regular morphology.…”