Preparation and characterization of electrospun nanofiber membrane from polyvinyl alcohol loaded with Glycyrrhiza glabra extract

Abstract

This study was designed to form an electrospun nanofiber membrane generated from different ratio of polyvinyl alcohol (PVA) and Glycyrrhiza glabra (licorice) extract. The electrospinning process was used to fabricate the nanofiber membrane using maximum amount of licorice extract solution. 90% licorice extract solution was successfully electrospun to fabricate nano membrane with 10% PVA solution. Antibacterial, cytotoxicity, scanning electron microscope (SEM) test were performed on resulted sample. The outstanding performance against Staphylococcus aureus ( S. aureus) by forming inhibition zone of 15 mm was observed. On the vero cell line, cell cytotoxicity was found, with only a few numbers of cells (less than 5%) surviving after exposure to the material. SEM investigation reveals fiber production with a diameter of (245.54 ± 55.19) nm on average. Moreover, Fourier transforms infrared spectroscopy (FT-IR) and moisture management test were performed. FT-IR spectra analysis confirmed the presence of licorice components in the membrane. Moisture analysis proved it as a better absorbing material. Since the fabricated membrane were proven to have bacteriostatic activity, it can be employed as wound healing.

Antibacterial multicomponent electrospun nanofibrous mat through the synergistic effect of biopolymers

“…Because, previous studies regarding natural polymer based nanofibrous mat showed average diameter range 175–245 nm and so on. 9,12,60…”

“…In addition, a previous study was carried out to assess the biocompatibility of PVA/licorice nanofibrous mat and the result showed good biocompatibility of the developed mat. 60 To know the actual activities of the chemical compounds of licorice extracts and chitosan against both bacteria (gram-negative and gram-positive), further studies are required.…”

“…This finding is consistent with previous research on the antibacterial characteristics of licorice and chitosan reported by several researchers. 12,60 Although electrospun nanofibrous mat from PVA/chitosan/neem extract showed ZOI 14.5 mm. 65 But, the addition of licorice extract with PVA and chitosan enhances the ZOI against the bacteria S. aureus which indicates the contribution of licorice extract for enhancing bacterial resistance.…”

“…Because, previous studies regarding natural polymer based nanofibrous mat showed average diameter range 175-245 nm and so on. 9,12,60 Functional groups of licorice, chitosan, collagen and PVA polymer in PCCLNM sample have been identified by infrared spectroscopy. From Figure 2 it is observed that the range of functional group region is 4000-698 cm −1 and the fingerprint region belongs to the range of 1000-698 cm −1 .…”

Electrospun Poly (Vinyl Alcohol) Nanofibrous Mat Loaded with Green Propolis Extract, Chitosan and Nystatin as an Innovative Wound Dressing Material

“…Ziziphus jujube Mill. [13], Aloe vera [14] and Glycyrrhiza glabra [15]), non-steroidal antiinflammatory drug (sodium salicylate, diclofenac sodium, indomethacin and naproxen) for transdermal drug delivery, and to improve the solubility of the poorly water-soluble drug Probucol [16].…”