Preparation and Characterization of Electrospun Collagen Based Composites for Biomedical Applications

Drobotă, Mioara; Grădinaru, Luiza Madalina; Vlad, Stelian; Bargan, Alexandra; Butnaru, Maria; Angheloiu, Marian; Aflori, Magdalena

doi:10.3390/ma13183961

Cited by 18 publications

(12 citation statements)

References 65 publications

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“…The amide I band correlated to the stretching vibration of the carbonyl groups was recorded at 1634.4 cm −1 (HK) and 1634.5 cm −1 (VALKE) for the β keratin sheets and at 1640.6 cm −1 (PRO), 1644.8 cm −1 (VALKE), and 1645 cm −1 (ESPE) for α keratin [ 19 , 20 ]. The amide II band was attributed to the N–H bending vibration and C–N stretching vibrations and was recorded at 1543.6–1558.6 cm −1 .…”

Section: Resultsmentioning

confidence: 99%

“…Amide I band’s deconvolution was performed in order to identify the ordered structures affected by enzymatic hydrolyses and to correlate with the bioactive properties. Thus, β-sheets, random coils, and α-helix were examined in the ranges of 1613–1637 cm − 1 , 1637–1645 cm − 1 , and 1645–1662 cm − 1 , respectively [ 19 ]. The deconvolution parameters were settled in the spectra range of 1700–1600 cm − 1 using a Lorrentian function.…”

Section: Methodsmentioning

confidence: 99%

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Wool Keratin Hydrolysates for Bioactive Additives Preparation

et al. 2021

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The aim of this paper was to select keratin hydrolysate with bioactive properties by using the enzymatic hydrolysis of wool. Different proteolytic enzymes such as Protamex, Esperase, and Valkerase were used to break keratin molecules in light of bioactive additive preparation. The enzymatic keratin hydrolysates were assessed in terms of the physico-chemical characteristics related to the content of dry substance, total nitrogen, keratin, ash, cysteic sulphur, and cysteine. The influence of enzymatic hydrolysis on molecular weight and amino acid composition was determined by gel permeation chromatography (GPC) and gas chromatography-mass spectrometry (GC-MS) analyses. Antimicrobial activity of keratin hydrolysates was analysed against Fusarium spp., a pathogenic fungus that can decrease the quality of plants. The bioactivity of enzymatic hydrolysates was tested on maize plants and allowed us to select the keratin hydrolysates processed with the Esperase and Valkerase enzymes. The ratio of organised structures of hydrolysate peptides was analysed by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) deconvolution of the amide I band and may explain the difference in their bioactive behaviour. The most important modifications in the ATR spectra of maize leaves in correlation with the experimentally proven performance on maize development by plant length and chlorophyll index quantification were detailed. The potential of enzymatic hydrolysis to design additives with different bioactivity was shown in the case of plant growth stimulation.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Wool Keratin Hydrolysates for Bioactive Additives Preparation

et al. 2021

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“…Inclusion was determined by relevance to the topic (i.e., must examine collagen-containing electrospun materials for tissue engineering/regenerative medicine applications) ( Figure 1 ). After assessment [ 16 , 17 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ,…”

Section: Methodsmentioning

confidence: 99%

Collagen-Based Electrospun Materials for Tissue Engineering: A Systematic Review

Blackstone

Powell

2021

Bioengineering

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Collagen is a key component of the extracellular matrix (ECM) in organs and tissues throughout the body and is used for many tissue engineering applications. Electrospinning of collagen can produce scaffolds in a wide variety of shapes, fiber diameters and porosities to match that of the native ECM. This systematic review aims to pool data from available manuscripts on electrospun collagen and tissue engineering to provide insight into the connection between source material, solvent, crosslinking method and functional outcomes. D-banding was most often observed in electrospun collagen formed using collagen type I isolated from calfskin, often isolated within the laboratory, with short solution solubilization times. All physical and chemical methods of crosslinking utilized imparted resistance to degradation and increased strength. Cytotoxicity was observed at high concentrations of crosslinking agents and when abbreviated rinsing protocols were utilized. Collagen and collagen-based scaffolds were capable of forming engineered tissues in vitro and in vivo with high similarity to the native structures.

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“…Although the cell viability slightly decreased after 48 h, the percentages were maintained above 80% (non-cytotoxic effect) for all samples, except Alg-Col-ZnONPs (3.98%) (p < 0.01). The coated Alg-Col nanostructure shows the absorption peaks at 1205 cm −1 (amide III, stretching vibration of C-H), 1538 cm −1 (amide II band) and 1643 cm −1 associated with the amide I (random coils) (Figure 4b) [51]. The absorption peaks at 1404 and 1449 cm −1 correspond to pyrrolidine ring vibration of hydroxyproline and proline [52].…”

Section: In Vitro Cytotoxicity Evaluationmentioning

confidence: 99%

Sustainable Coated Nanostructures Based on Alginate and Electrospun Collagen Loaded with Antimicrobial Agents

et al. 2021

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In this study, sodium alginate film (Alg) was coated with electrospun collagen glue (Col) extracted from rabbit skin waste, loaded with different commercial antimicrobial agents (chitosan, AG425K and ZnONPs) and investigated in terms of morphological, structural and biological properties. The coated nanostructures were characterized using scanning electron microscopy coupled with the energy-dispersive X-ray (SEM/EDS), Attenuated Total Reflectance Fourier-Transform Infrared spectroscopy (ATR FT-IR), and Atomic Force Microscopy (AFM) tests. The cytotoxicity was investigated on murine L929 fibroblasts using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide salt (MTT) and lactate dehydrogenase (LDH) assays. Microbiological tests were performed against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 27853 standard strains. In vitro cell culture tests showed a good cytocompatibility of the coated nanostructured systems, except the sample loaded with ZnONPs, which exhibited a highly cytotoxic effect. Alg-Col-ZnONPs nanostructure inhibited the growth and multiplication of the Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 bacterial strains. The results of new coated nanostructures may be useful for the development of sustainable biomaterials in a circular economy, with bioactive properties for medical wound dressings.

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Preparation and Characterization of Electrospun Collagen Based Composites for Biomedical Applications

Cited by 18 publications

References 65 publications

Wool Keratin Hydrolysates for Bioactive Additives Preparation

Wool Keratin Hydrolysates for Bioactive Additives Preparation

Collagen-Based Electrospun Materials for Tissue Engineering: A Systematic Review

Sustainable Coated Nanostructures Based on Alginate and Electrospun Collagen Loaded with Antimicrobial Agents

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