Preparation and Characterization of Fish Skin Collagen Material Modified with β-Glucan as Potential Wound Dressing

Abstract

Collagen possesses unique properties, e.g., biocompatibility, biodegradability, and non-toxicity. However, collagen material degrades too quickly and has low mechanical properties. One of the methods of polymers’ modification is mixing them to obtain blends. In this study, the influence of β-glucan for collagen material was analyzed. The interaction between the functional groups of the polymer was analyzed by ATR-FTIR (attenuated total reflection-fourier transform infrared) spectroscopy. The influence of β-glucan on mechanical properties was evaluated. The surface properties of materials were assessed using contact angle measurements and the topography of materials was evaluated by AFM (atomic force microscope). The structure of materials was analyzed according to SEM (scanning electron microscopy) pictures. Moreover, the DPPH-free radicals’ scavenging ability and biocompatibility against erythrocytes and HaCaT cells were evaluated. Collagen and β-glucan were bound together by a hydrogen bond. β-glucan addition increased the roughness of the surface of the film and resulted in a more rigid character of the materials. A small addition of β-glucan to collagen provided a more hydrophilic character. All the materials could swell in in vitro conditions and showed antioxidant activity. Materials do not cause erythrocyte hemolysis. Finely, our cytotoxicity studies indicated that β-glucan can be safely added at small (10% or less) quantity to collagen matrix, they sufficiently support cell growth, and the degradation products of such matrices may actually provide some beneficial effects to the surrounding cells/tissues.

Characterization of Collagen/Beta Glucan Hydrogels Crosslinked with Tannic Acid

“…Collagen was isolated from the skin of Hypophthalmichthys nobilis— a by-product from the food industry. Collagen was obtained according to the methodology described in our previous work [ 14 ]. Shortly, the skin was cut into small pieces and cleaned with distilled water.…”

“…Collagen and β-glucan solutions were mixed in a 90/10 w/w ratio. This composition was chosen based on our previous study [ 14 ]. The collagen/β-glucan mixture was modified by using 2, 5, and 10% tannic acid addition.…”

“…This method can be used to quantify antioxidants in complex biological systems for both solid and liquid samples [ 18 ]. In the presence of a free radical acceptor, the dark purple color of the solution changes to yellow, thanks to which it is possible to measure the samples with a spectrophotometer [ 14 ]. A solution of 250 µM DPPH with methanol as a solvent was prepared.…”

An important polysaccharide from fermentum

“…This wound dressing allows to simplify the steps of wound care and significantly reduces the pain after an injury. Moreover, it can fully promote skin cell growth during application ( Michalska-Sionkowska et al, 2021 ).…”

Application of marine collagen for stem‑cell‑based therapy and tissue regeneration (Review)

“…In recent years, fish collagen had been increasingly used for wound dressing applications due to their low antigenicity, biodegradability and biocompatibility ( 84-86 ). Recently, collagen extracted from Hypophthalmichthys nobilis skin has been used to serve as the main component for wound dressing, which has been found to have good swelling properties in in vitro settings and exhibits antioxidant activity ( 87 ). In addition, this type of wound dressing does not cause erythrocyte hemolysis and sufficiently supports cell proliferation ( 87 ).…”