Preparation and characterization of gold nanoparticles capped by peptide–biphenyl hybrids

Pérez, Yolanda; Mann, Enrique; Herradón, Bernardo

doi:10.1016/j.jcis.2011.04.029

Cited by 13 publications

(9 citation statements)

References 111 publications

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“…After that, de-alloying was done by nitric acid [36]. Gold nanoparticles was prepared by microwave irradiation method in which aqueous extracts of Cissus quadrangularis (CQE) was used as capping agent or reducing agent [37].…”

Section: Physical Methodsmentioning

confidence: 99%

Gold Nanoparticles: Synthesis and Applications in Drug Delivery

Ak¹,

Rashid²,

Murtaza³

et al. 2014

Trop. J. Pharm Res

385

163

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This review is focused essentially on the synthesis and applications of gold nanoparticles in the field of medicine and targeted drug delivery. Nanotechnology has become one of the most interesting and advanced areas of research in this field. Among nanoparticles, gold nanoparticles demonstrate special advantages in this field due to their unique properties, small size and high surface area-to-volume ratio.These particles have been widely used in various biomedical applications and drug delivery systems due to their inert nature, stability, high dispersity, non-cytotoxicity and biocompatibility.

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Section: Physical Methodsmentioning

confidence: 99%

Gold Nanoparticles: Synthesis and Applications in Drug Delivery

Ak¹,

Rashid²,

Murtaza³

et al. 2014

Trop. J. Pharm Res

385

163

View full text Add to dashboard Cite

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“…In a previous study [9], we used PBHs containing cysteine (Cys), tyrosine (Tyr) and glycine (Gly) amino acids to form stable AuNPs: Au[(TrCys) 2 B] and [(Gly-Tyr-TrCys) 2 B]. In the present study, we demonstrate that the amino acids methionine (Met) and tryptophan (Trp) are also useful to prepare stable functionalised AuNPs such as Au[(Met) 2 B], Au[(Gly-Tyr-Met) 2 B] and Au[(Gly-Trp-Met) 2 B].…”

Section: Resultsmentioning

confidence: 99%

“…Five AuNPs, (Au[(Gly-Trp-Met) 2 B], Au[(Gly-Tyr-TrCys) 2 B], Au[(Gly-Tyr-Met) 2 B], Au[(Met) 2 B] and Au[(TrCys) 2 B]) (Figure 1), were synthesised following the methodology described by Pérez et al [9] (see Additional file 1). Thus, each PBH (50 μmol) was dissolved in ethanol (20 ml, 2.5 mmol/l) and was added to a solution of HAuCl 4 (50 ml, 0.5 mmol/l) in 2-propanol under stirring.…”

Section: Methodsmentioning

confidence: 99%

“…These observations highlight the use of capping agents as an approach to achieve safer NPs. We recently proposed the use of peptide-biphenyl hybrid (PBH) ligands as capping agents [9]. PBHs have a biphenyl system and two amino acid/peptide fragments, and they present key characteristics, such as dynamic properties in solution [10], ordered structures in the solid phase [11] and biological activity as calpain inhibitors [12].…”

Section: Introductionmentioning

confidence: 99%

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Peptide-biphenyl hybrid-capped AuNPs: stability and biocompatibility under cell culture conditions

et al. 2013

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In this study, we explored the biocompatibility of Au nanoparticles (NPs) capped with peptide-biphenyl hybrid (PBH) ligands containing glycine (Gly), cysteine (Cys), tyrosine (Tyr), tryptophan (Trp) and methionine (Met) amino acids in the human hepatocellular carcinoma cell line Hep G2. Five AuNPs, Au[(Gly-Tyr-Met)2B], Au[(Gly-Trp-Met)2B], Au[(Met)2B], Au[(Gly-Tyr-TrCys)2B] and Au[(TrCys)2B], were synthesised. Physico-chemical and cytotoxic properties were thoroughly studied. Transmission electron micrographs showed isolated near-spherical nanoparticles with diameters of 1.5, 1.6, 2.3, 1.8 and 2.3 nm, respectively. Dynamic light scattering evidenced the high stability of suspensions in Milli-Q water and culture medium, particularly when supplemented with serum, showing in all cases a tendency to form agglomerates with diameters approximately 200 nm. In the cytotoxicity studies, interference caused by AuNPs with some typical cytotoxicity assays was demonstrated; thus, only data obtained from the resazurin based assay were used. After 48-h incubation, only concentrations ≥50 μg/ml exhibited cytotoxicity. Such doses were also responsible for an increase in reactive oxygen species (ROS). Some differences were observed among the studied NPs. Of particular importance is the AuNPs capped with the PBH ligand (Gly-Tyr-TrCys)2B showing remarkable stability in culture medium, even in the absence of serum. Moreover, these AuNPs have unique biological effects on Hep G2 cells while showing low toxicity. The production of ROS along with supporting optical microscopy images suggests cellular interaction/uptake of these particular AuNPs. Future research efforts should further test this hypothesis, as such interaction/uptake is highly relevant in drug delivery systems.

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“…Investigations of citrate capped gold nanoparticles showed that they have a negatively charged surface and preferentially bind to thiol, amine and cyanide functional groups [11]. In order to further stabilize and modulate their physico-chemical properties, gold nanoparticles have been functionalized with a wide range of simple and complex molecules by means of their reactive functional groups [12]. For example, peptides [13], proteins [14], DNA [15], polymers [16], and dendrimers [17] were conjugated to the gold nanoparticles surface by means of non-covalent electrostatic interaction, hydrophobic interaction and covalent binding [12, 14].…”

Section: Introductionmentioning

confidence: 99%

Rational design of gold nanocarrier for the delivery of JAG-1 peptide

et al. 2015

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BackgroundUnique properties exhibited by nanoparticles makes them great candidates for applications in physics, chemistry, biology, material science and medicine. The biological applications of water-soluble gold nanoparticles range from contrast agents, delivery vehicles to therapeutics. Notch signaling is a complex network that orchestrates cell fate decisions, which involves proliferation, migration, differentiation and cell death in organisms ranging from insects to humans. Studies have showed that a correct orientation of the Jag-1 signalling protein on the substrates proves to be of great importance when promoting Jagged-1 Notch interactions, also the availability of the ligands, super cedes the importance of their concentration.ResultsThe aim of the present study was to synthetize a Jag-1 functionalized nanocarrier, which would promote an efficient interaction between the Jag-1 peptide and the Notch receptor. To this end, two routes for gold nanoparticle-peptide assembly were investigated, and the synthetized bio-nanostructures were characterized and compared by means of UV–Vis, FT-IR, DLS and AFM techniques.ConclusionsWe have obtained a stable, monodisperse, hetero-functionalized GNP-PEG-JAG-1 bio-nanostructure for Notch pathway activation applications.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-015-0100-x) contains supplementary material, which is available to authorized users.

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Preparation and characterization of gold nanoparticles capped by peptide–biphenyl hybrids

Cited by 13 publications

References 111 publications

Gold Nanoparticles: Synthesis and Applications in Drug Delivery

Gold Nanoparticles: Synthesis and Applications in Drug Delivery

Peptide-biphenyl hybrid-capped AuNPs: stability and biocompatibility under cell culture conditions

Rational design of gold nanocarrier for the delivery of JAG-1 peptide

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