Preparation and characterization of human keratinocyte–fibroblast cell sheets constructed using PNIAM-co-AM grafted surfaces for burn wound healing

Benchaprathanphorn, Kanokaon; Sakulaue, Phongphot; Siriwatwechakul, Wanwipa; Muangman, Pornprom; Chinaroonchai, Kusuma; Viravaidya-Pasuwat, Kwanchanok

doi:10.1007/s10856-020-06469-4

Cited by 8 publications

(12 citation statements)

References 40 publications

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“…The major obstacle to using skin cell sheets in clinical practices is their size limit 5 . In real applications, the skin cell sheets need to be at least ten times larger than their current size for full wound area coverage to promote effective treatment 6,7 .…”

Section: Discussionmentioning

confidence: 99%

“…In the previous work, we had developed an in-house method to fabricate temperature-responsive surfaces for cell sheet construction using PNIAM-co-AM graft-copolymer by UV irradiation 3,4 . The cell sheets fabricated by PNIAM-co-AM coated plates possessed the physical and biological properties similar to those constructed by using UpCell™ plates including the cell viability, the size of the harvested sheets and the protein expression 5 . Besides signi cant cost reduction in the cultureware, by using the co-polymer method, it allowed us to construct various shapes and sizes of cell sheets.…”

Section: Introductionmentioning

confidence: 92%

“…However, a signi cantly large number of cells are required for the cell sheet construction, leading to a long period of cell expansion, so increasing the risks and complications during hospitalization. When the cell sheets are detached from the temperature-responsive culture surfaces, they undergo cytoskeletal re-organization 8, 9 , causing the cell sheet shrinkage resulting in practically a 40% size reduction 5 .…”

Section: Introductionmentioning

confidence: 99%

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Expansion of Fibroblast Cell Sheets Using a Modified MEEK Micrografting Technique for Wound Healing Applications

Benchaprathanphorn

Sakulaue

Siriwatwechakul

et al. 2022

Preprint

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One of many challenges in wound treatment is to provide sufficient wound area coverage to protect against pathogens and speed up the wound healing process. Recently, cell sheet engineering has shown promise in treating deep wounds. Unfortunately, the cell sheet size is too small for practical use. To overcome this limitation, the MEEK micrografting technique, currently being used in the treatment of extensive deep burns, was investigated. This technique was modified and applied to enlarge dermal fibroblast cell sheets, constructed using temperature-responsive PNIAM-co-AM graft copolymer, by transforming the intact cell sheets into small cell islands at expansion ratios of 1:3, 1:6 and 1:9. Afterwards, cell mechanisms essential for wound healing, including reattachment, proliferation, and migration were investigated. The fibroblast cells on MEEK gauzes possessed high cell viability, capable of reattachment and migration. The optimal expansion ratio having the highest migration rate was 1:6, possibly due to an effective intercellular communication distance. Therefore, the combination of cell sheet engineering with the MEEK micrografting technique could provide high quality cells with a large coverage area, which would be particularly beneficial in wound care applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Expansion of Fibroblast Cell Sheets Using a Modified MEEK Micrografting Technique for Wound Healing Applications

Benchaprathanphorn

Sakulaue

Siriwatwechakul

et al. 2022

Preprint

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“…Other research, such as that reported by Jyoti et al, focused on burn wounds for which chitosan has been widely employed as an assistant for fusidic acid [ 19 ]. Benchaprathanphorn et al also tested poly(N-isopropryl acrylamide- co -acrylamide) (PNIPAAm- co -Am) to fabricate a keratinocyte-fibroblast tissue for treating burn wounds, which showed cell migration and an organization similar to skin tissue’s structure [ 20 ]. Both approaches are considered as positive and improve wound healing rate.…”

Section: Introductionmentioning

confidence: 99%

Exploration of Bioengineered Scaffolds Composed of Thermo-Responsive Polymers for Drug Delivery in Wound Healing

Henríquez

Castro-Alpízar

Lopretti-Correa

et al. 2021

IJMS

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Innate and adaptive immune responses lead to wound healing by regulating a complex series of events promoting cellular cross-talk. An inflammatory response is presented with its characteristic clinical symptoms: heat, pain, redness, and swelling. Some smart thermo-responsive polymers like chitosan, polyvinylpyrrolidone, alginate, and poly(ε-caprolactone) can be used to create biocompatible and biodegradable scaffolds. These processed thermo-responsive biomaterials possess 3D architectures similar to human structures, providing physical support for cell growth and tissue regeneration. Furthermore, these structures are used as novel drug delivery systems. Locally heated tumors above the polymer lower the critical solution temperature and can induce its conversion into a hydrophobic form by an entropy-driven process, enhancing drug release. When the thermal stimulus is gone, drug release is reduced due to the swelling of the material. As a result, these systems can contribute to the wound healing process in accelerating tissue healing, avoiding large scar tissue, regulating the inflammatory response, and protecting from bacterial infections. This paper integrates the relevant reported contributions of bioengineered scaffolds composed of smart thermo-responsive polymers for drug delivery applications in wound healing. Therefore, we present a comprehensive review that aims to demonstrate these systems’ capacity to provide spatially and temporally controlled release strategies for one or more drugs used in wound healing. In this sense, the novel manufacturing techniques of 3D printing and electrospinning are explored for the tuning of their physicochemical properties to adjust therapies according to patient convenience and reduce drug toxicity and side effects.

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“…3 The cell sheets consisting of various types of dense populations of cells are formed on special surfaces without the need for scaffolding material and the preliminary results reported very successful regeneration capacity. [4][5][6] In this sense, cell sheets have shown tremendous promise in the last decade for the repair or restoration of weakened tissues or damaged organs. 7 The promising pre-clinical and clinical applications of cell sheets in corneal reconstruction, cardiac graft, osteochondral regeneration, wound healing, and oesophageal mucosa graft have been reported up to date.…”

Section: Introductionmentioning

confidence: 99%

Multi-layered in vitro 3D-bone model via combination of osteogenic cell sheets with electrospun membrane interlayer

Tevlek

Aydın

2021

J Biomater Appl

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In this study, it was aimed to present an approach for the development of multi-layered tissue engineering constructs by using cell sheet engineering. Briefly, MC3T3-E1 mouse pre-osteoblast cells were cultured in temperature-responsive plates (Nunc Upcell®) in the presence of osteogenic medium and the resulting cell sheets were laminated with electrospun poly(L-lactic acid) (PLLA) membranes to obtain viable three-dimensional, thick constructs. The constructs prepared without PLLA membranes were used as control. The cell viability and death in the resulting structures were investigated by microscopic and colorimetric methods. The in vitro performance of the structures was discussed comparatively. Alkaline phosphatase (ALP) activity, collagen and sulfated glycosaminoglycan (sGAG) content values were calculated. The presented approach shows potential for engineering applications of complex tissues with at least two or more microenvironments such as osteochondral, corneal or vascular tissues.

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Preparation and characterization of human keratinocyte–fibroblast cell sheets constructed using PNIAM-co-AM grafted surfaces for burn wound healing

Cited by 8 publications

References 40 publications

Expansion of Fibroblast Cell Sheets Using a Modified MEEK Micrografting Technique for Wound Healing Applications

Expansion of Fibroblast Cell Sheets Using a Modified MEEK Micrografting Technique for Wound Healing Applications

Exploration of Bioengineered Scaffolds Composed of Thermo-Responsive Polymers for Drug Delivery in Wound Healing

Multi-layered in vitro 3D-bone model via combination of osteogenic cell sheets with electrospun membrane interlayer

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