Preparation and characterization of magnetic gene vectors for targeting gene delivery

Zheng, Songming; Liu, G.; Hong, Ruoyu; Li, H.Z.; Li, Y.G.; Wei, Dongguang

doi:10.1016/j.apsusc.2012.07.019

Cited by 20 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When MNPs radius is larger than R max in equation ( 6), magnetic force is strong enough by itself to overcome the membrane deformation cost, which means that internalized pathway becomes magnetically controlled. Interestingly, the modeling results are well consistent with recent experimental observations [26,29,[41][42][43][44]. Our theoretical results show the optimal radius of spherical NPs at 26 nm without magnetic field (as shown in figure 4), which is in good agreement with experimental detections that the NPs with radius about 25 nm have a maximal endocytic rate [41,42].…”

Section: Resultssupporting

confidence: 91%

Towards a better understanding of the effects of the magnetic nanoparticles size and magnetic field on cellular endocytosis

Sun²,

Chen

et al. 2020

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

We present a thermodynamic approach to elucidate the effects of the size of magnetic nanoparticles (MNPs), intensity of magnetic field and biologic parameters on endocytosis under a magnetic field. It is found that the external magnetic field not only results in the increase of endocytic rate, but also can alter the internalized pathway. The minimum and maximum radius for receptor-mediated endocytosis can be calculated by simple straightforward expressions. Furthermore, we constructed a phase diagram to clarify the interrelated effects of MNP radius and magnetic field on endocytosis rate. We can identify from the phase the relations among the size of MNPs, intensity of magnetic field and endocytosis rate. Our theoretical findings can provide useful guidance to the design of MNPs for diagnostic agents and drug delivery applications.

show abstract

Section: Resultssupporting

confidence: 91%

Towards a better understanding of the effects of the magnetic nanoparticles size and magnetic field on cellular endocytosis

Sun²,

Chen

et al. 2020

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

show abstract

“…As shown in Figure 10, with the assistance of a magnetic field, more green fluorescence dots were observed in cells, which confirmed that the external magnetic field could increase the cellular uptake. As in previous studies, the cellular endocytosis of magnetic nanoparticles can be enhanced in the presence of an external magnetic field [37,39]. Our result is in accordance with the literatures.…”

Section: In Vitro Cytotoxicitysupporting

confidence: 94%

Synthesis, Characterization, and Evaluation of Disulfide-Containing Polyethylenimine Derivative Functionalized Magnetic Carbon Nanotubes as an Efficient Gene Vector

Qiao

Lei

et al. 2019

Journal of Nanomaterials

View full text Add to dashboard Cite

Magnetic nanoparticles have been widely developed as vectors in targeting drug and gene delivery. Disulfide-containing polyethylenimine derivative- (SSPEI-) functionalized magnetic carbon nanotubes (CNTs/Fe3O4-SSPEI) were synthesized as gene vector. Fourier transform infrared, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and thermogravimetric analysis were used to characterize CNTs/Fe3O4-SSPEI nanoparticles. The magnetic nanoparticles displayed typical superparamagnetic behavior and excellent dispersibility in water. Plasmid DNA could be bound by CNTs/Fe3O4-SSPEI to form the complexes. The sizes of complexes are about 400 nm, and the zeta potentials are positive at the w/w ratio over 6. CNTs/Fe3O4-SSPEI nanoparticles displayed higher transfection activity than did PEI (25 kDa), whereas the cytotoxicity was rather lower. Moreover, the transfection efficiency was further increased with the assistance of an external magnetic field. These results indicate that CNTs/Fe3O4-SSPEI nanoparticles would be a promising vector in targeted gene delivery.

show abstract

“…Similarly, Chen et al [147] combined double ligand targeting (c(RGDyK) and DSPE-PEG2000) with magnetic targeting to meet the clinical application needs. The results showed that the nanoparticles effectively overcome the problem that magnetic fluid cannot be injected intravenously; this is because of the fact that the dual targeted ligands can effectively locate the nanoparticles to the tumor site [148].…”

Section: Other Classesmentioning

confidence: 99%

Progress in magnetic Fe₃O₄nanomaterials in magnetic resonance imaging

Yang

Hui

et al. 2020

Nanotechnology Reviews

View full text Add to dashboard Cite

At present, high-sensitivity, high-penetration-depth, and accurate tissue resolution clinical imaging effect are required, while computer transverse scanning, microwave imaging, and fluorescence imaging (FL) cannot meet the requirements of clinical imaging, but the magnetic resonance imaging (MRI) can meet the requirements of clinical dissecting details. The effect of MRI imaging is closely related to the contrast agent (CA). As an important type of CA, Fe3O4 and its analogues have been widely concerned because of their low toxicity and relatively low price. In this review, we summarize the development and improvement of CAs based on Fe3O4 and its analogues from T 2 imaging mode and development limitation in the initial single modulus imaging mode, to T 1 imaging mode overcoming the limitations of T 2 imaging and the limitations of its own in application, to the later development of dual modulus imaging form, and to the current multi-modulus imaging form. Simultaneously, we demonstrate the research progress, preparation methods, and future trends based on Fe3O4 and its analogues CAs for MRI, the current application status is preliminarily summarized, and the future development trend is prospected.

show abstract

Preparation and characterization of magnetic gene vectors for targeting gene delivery

Cited by 20 publications

References 21 publications

Towards a better understanding of the effects of the magnetic nanoparticles size and magnetic field on cellular endocytosis

Towards a better understanding of the effects of the magnetic nanoparticles size and magnetic field on cellular endocytosis

Synthesis, Characterization, and Evaluation of Disulfide-Containing Polyethylenimine Derivative Functionalized Magnetic Carbon Nanotubes as an Efficient Gene Vector

Progress in magnetic Fe₃O₄nanomaterials in magnetic resonance imaging

Contact Info

Product

Resources

About

Preparation and characterization of magnetic gene vectors for targeting gene delivery

Cited by 20 publications

References 21 publications

Towards a better understanding of the effects of the magnetic nanoparticles size and magnetic field on cellular endocytosis

Towards a better understanding of the effects of the magnetic nanoparticles size and magnetic field on cellular endocytosis

Synthesis, Characterization, and Evaluation of Disulfide-Containing Polyethylenimine Derivative Functionalized Magnetic Carbon Nanotubes as an Efficient Gene Vector

Progress in magnetic Fe3O4nanomaterials in magnetic resonance imaging

Contact Info

Product

Resources

About

Progress in magnetic Fe₃O₄nanomaterials in magnetic resonance imaging