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Applications in wearable and implantable electronic information products have increased in recent years, and traditional conductive materials have poor flexibility. To meet the trend of flexible, wearable and implantable electronic devices, flexible conductive materials are widely accepted. Here, we report on a method of preparing a flexible conductive textile material. The graphene is finished onto the surface of the silk fabric by multiple impregnation-reduction methods, and the silk fabric retains good flexibility while obtaining conductivity. Flexible conductive silk also has a good temperature and strain response, and its electrical resistance changes with temperature and strain. The obtained flexible conductive silk fabric has the advantages of both silk fabric and graphene, it has broad application prospects in wearable devices, health care monitoring, and human-machine interface.