“…48 According to these spectra, free microbial consortium contained functional groups such as -NH, C-N, P]O, -CH 2 , P-OH, P-O-C and -COOH which indicated that bacterial cells contain polysaccharides, nucleotide and protein; 40 the immobilized control had -NH, -CH 2 , C]C, -COOH, mineral crystal compounds and Fe 3 O 4 which suggested that semi-coke, sodium alginate and lactose included mineral crystal and metallic compounds, and organism substance; the immobilized microbial consortium had -NH, -OH, C-N, C]C, -COOH, P]O, mineral crystal compounds and Fe 3 O 4 which was not the merged one. 48 Compared to them, through immobilizationtreatment, asymmetric and symmetric deformation of -CH 2 groups was absent in the spectrum which probably proved that -CH 2 groups changed into carbonate; P-OH and P-O-C translated into P]O; -OH present in the spectrum. Xu et al 40 thought immobilization prevents loss or damage of some molecular groups.…”