Preparation and characterization of mycobacterial antigen-specific T-cell clones from mice with genetically different sensitivity to tuberculosis

Еремеев, В. В.; Lyadova, Irina V.; Майоров, К. Б.; Хайдуков, С. В.; Moroz, A. M.; Apt, Alexander S.

doi:10.1007/bf02766191

Cited by 1 publication

(1 citation statement)

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“…Two T cell clones with different antigen specificity were used. A1G10, I-A k -restricted, PPD-specific, T clone was obtained in our laboratory from PPD-immune B10.A (H-2 a ) lymph node cells [21]. D10.G4, I-A k -restricted, conalbumin-specific Th2 clone was a kind gift from Dr V. Yurin (Institute for Genetics of Microorganisms, Moscow, Russia).…”

Section: Antigen Presentation To T Cell Clonesmentioning

confidence: 99%

Influence of the mouse Bcg Tbc-1 and xid genes on resistance and immune responses to tuberculosis infection and efficacy of bacille Calmette–Guérin (BCG) vaccination

Никоненко

Apt

Mezhlumova

et al. 1996

Clinical and Experimental Immunology

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SUMMARYWe have studied the role of three mouse distinct non-H-2 genes (Bcg, Tbc-1, xid ) in several phenomena of antituberculosis immunity and resistance. On the basis of median survival time (MST) of mice following infection with virulent Mycobacterium tuberculosis H37Rv, Bcg gene did not control resistance to the lethal dose of H37Rv infection in non-vaccinated and Myco. bovis (BCG)-vaccinated mice. However, Bcg r allele, in comparison with Bcg s allele, determined more effective suppression of an early multiplication in spleens of H37Rv mycobacteria after a low dose (5 2 10 4 colony-forming units (CFU)) injection. CBA/N mice, which are not protected efficiently against tuberculous challenge by BCG vaccination, were characterized by a decreased in vitro proliferation of immune lymph node cells, both spontaneous and stimulated with mycobacterial antigens. The decreased proliferation was due to immunosuppression caused by interactions between responding T cells and CBA/N antigen-presenting cells (APC). We have confirmed that the defective response to BCG-vaccination in CBA/N mice is linked with the X-chromosome and thus is presumably determined by the xid gene itself. I/St mice (Tbc-1 s ), supersusceptible to H37Rv infection, were not able to restrict the growth of H37Rv mycobacteria in spleens, even following infection with a low dose (5 2 10 4 ), but restricted the growth of Myco. bovis BCG more effectively than Bcg s mice.

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Section: Antigen Presentation To T Cell Clonesmentioning

confidence: 99%