Preparation And Characterization Of Natural Nano Hydroxyapatite From Eggshell And Seashell And Its Effect On Bone Healing

Abstract

The Objectives of the present study were undertaken to prepared hydroxyapatite powder from eggshell and seashell and convert them in Nano size. Hydroxyapatite (HA) is a biomaterial with osteoconductive properties and is prepared by the precipitation method. The properties of HA can be enhanced when the material is synthesized in the Nanoscale range to use in the mandibular bone of the rabbit to examine the influence of (HA) on bone healing.The eggshell and seashell were cleaned, and the phosphoric acid was added. The product inserts in the oven then calcined at 700˚C for 1 hour in the muffled furnace to evaporate CO 2 and to get the white crystalline powder, which indicated the presence of HA, this powder was converting to nanoparticle by attraction method. The product was applied in the groove of the rabbit's mandibular bone after surgical procedures to show the bone healing in histological examination. The results of IR Spectroscopy for the eggshell and seashell hydroxyapatite powder showed that the two compounds showed the same IR spectrum and the location of bands is approximately the same in the two compounds compared with the commercial hydroxyapatite powder by using FT-IR spectroscopy after dried at 100˚C. Histologically show that eggshell and seashell hydroxyapatite powder accelerated the bone healing in the mandibular bone of the rabbits. It can be concluded that, hydroxyapatite nanoparticles can successfully be produced by chemical precipitation techniques from eggshells and seashells with a phosphoric acid solution. Eggshell and seashell hydroxyapatite powder was used successfully in bone healing ‫ـــــــــــــــــــــــــــــــــــــــــ‬

Novel Trends into the Development of Natural Hydroxyapatite-Based Polymeric Composites for Bone Tissue Engineering

“…Some trace elements do not modify the elementary crystallographic properties of HA but may improve the general biological performance of the material used for implants [ 141 ]. Alhussary et al applied nano-HA from seashells and eggshells to the damaged mandibular bone of rabbits and reasoned that HA obtained from eggshells has an improved osteoinductivity and porosity than the one obtained from seashells [ 142 ]. Furthermore, Waheed recommended that eggshell scaffolds have superior compressive strength, increased mineralization, and improved osteogenic differentiation potential compared to commercial HA [ 143 ].…”

Biogenic calcium carbonate derived from waste shells for advanced material applications: A review

“…However, plenty of research is available in the literature that used and reported the calcium carbonate derived biogenic waste in foods, cosmetics, and pharmaceutical industry by a simple direct method, which is out of the interest of the current review paper. There is also plenty of research in the literature that used the calcium carbonate derived biogenic waste as starting materials to obtain other calcium derivatives such as calcium citrate (Palacios et al, 2021), hydroxyapatite (Alhussary et al, 2020), and calcium lactate (Daengprok et al, 2002) for pharmaceutical and food industry applications.…”

Recent advances in green processing technologies for valorisation of eggshell waste for sustainable construction materials

“…Consequently, in recent times the valorisation of eggshells waste as an alternative raw material for production of value added-products is upsurging. For instance, eggshell waste has been used as an additive in calcium phosphate bio-ceramics [ 75 , 76 , 77 ], as calcium supplement [ 78 , 79 , 80 , 81 ], adsorbent material [ 57 , 82 , 83 ], as a solid catalyst for biodiesel production [ 84 , 85 , 86 , 87 ], bone graft substitute [ 28 , 88 , 89 , 90 ], environmental remediation [ 91 , 92 ], heavy metal removal [ 36 , 93 ]. However, the sole application of eggshells in food and industrial rely main on the calcite component for making fertilizer, medicines and building materials.…”