Preparation and Characterization of Protein-Loaded Electrospun Fiber Mat and Its Release Kinetics

Abstract

For the enhancement of protein's bioavailability, a specific delivery system was developed by coaxial electrospinning. Bovine serum albumin (BSA) was used as protein model, and the core-sheath fiber mat was fabricated using sodium alginate as shell layer and the BSA-loaded chitosan nanoparticle that was prepared previously as core layer. By optimizing electrospinning parameters, uniform fibers with diameters ranging from 200-600 nm were obtained, and transmission electron microscopy and confocal laser scanning microscopy revealed their core-sheath structures. Fourier transform infrared spectroscopy (FTIR) analysis demonstrated that there existed molecular interaction between components, which enhanced the mat's thermal stability and mechanic property. It was found that the predominant release mechanism of BSA from fiber mat was erosion, and little change occurred in the secondary structure of encapsulated BSA indicated by FTIR and circular dichroism analysis. The study shows that the obtained fiber mat is a potential delivery system for protein.

Tunable drug release from nanofibers coated with blank cellulose acetate layers fabricated using tri-axial electrospinning

“…The ability to fabricate structures with controllable nanoscale architectures has enabled the development of much new science and technology (Isaacoff & Brown, 2017), and is of vital importance in the development of new kinds of functional nanomaterials, particularly for biomedical fields (Hubbell & Chikoti, 2012;Mehta, et al, 2017;Haider, et al, 2018;Mitragotri, Burke, & Langer, 2014;Khoshnevisan, et al, 2018;Wen, et al, 2017). Beyond simple monolithic structures, where the composition is the same throughout, a range of more complicated nanostructures can be envisaged.…”

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

“…However, based on our literature survey, the scaffolds production with pure natural polymers as a shell and aqueous biological solutions without any additive as a core have not been reported elsewhere. In most cases, the natural polymers are blended with highly spinnable synthetic polymers as shell 29,[39][40][41][42] or the synthetic hydrophilic polymers [42][43][44] are added to aqueous biological solutions to facilitate forming of core-shell fibers. This could be due to the difficulties in matching the parameters that affect electrospining process.…”

Preparation and characterization of an electrospun colon-specific delivery system for salmon calcitonin

“…[18][19][20] It can be even used to develop a colon-targeted delivery system to achieve slow and sustained release of protein. 21 In order to improve its stability and bioavailability, a novel colon-specic delivery system for SCT was developed by electrospinning in this study. Pectin is an anionic polymer which is easily degraded by microbial enzymes in the colon, thus it was coated on the surface of liposomes to further enhance their stability.…”