The data reported in literature demonstrate that carbon paste electrodes (CPEs) are very suitable for a variety of applications and many works have thus been devoted in the development of new sensitive and selective electrode surfaces based on carbon paste as the electrode material of choice. The application of novel and promising carbonaceous materials, as electrode surfaces, is an issue of great concern. In this work the experimental results of the characterization and comparison of electrode surfaces based on alternatively prepared carbonaceous materials (activated carbon (B), HNO 3 oxidized activated carbon (B5), Ag impregnated activated carbon (B-Ag) and graphite oxide (GO), are being demonstrated. Scaning Electron Microscopy SEM), surface acidity, FTIR spectroscopy, XRD diffractometry and electrochemical techniques (cyclic voltammetry, differential pulse voltammetry) were applied in the characterization of novel carbonaceous materials aimed at electrochemical DNA sensing.