Preparation and characterization of temperature‐responsive chromatographic column containing poly(<i>N</i>‐isopropylacrylamide) and poly([2‐(methacryloyloxy)‐ ethyl]trimetylammonium chloride)

Dai, Rongji; Chen, Lei; Liu, Zongjian; Wang, Haihui; Hu, Dawei; Deng, Yulin

doi:10.1002/app.33830

Cited by 16 publications

(10 citation statements)

References 25 publications

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“…Alongside columns based on homo-PNIPAAm, several co-polymers including NIPAAm have been tested as well, as they lead to a modification of the LCST and induce slightly different retention properties. [35][36][37][38][39][40][41][42] Other polymers that have successfully been used in TR columns include polyoxazoline (LCST controllable) and polyvinylcaprolactam (LCST 35ºC). 43,44 The possibility of applying TRLC in heart-cutting 2D-LC (LC-LC) has recently been illustrated by Kanazawa et al, where a TR column was used as a pretreatment column.…”

Section: Temperature-responsive Chromatographymentioning

confidence: 99%

Enhancing the Possibilities of Comprehensive Two-Dimensional Liquid Chromatography through Hyphenation of Purely Aqueous Temperature-Responsive and Reversed-Phase Liquid Chromatography

Baert¹,

Martens²,

Desmet

et al. 2018

Anal. Chem.

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Comprehensive two-dimensional liquid chromatography (LC × LC) allows for substantial gains in theoretical peak capacity in the field of liquid chromatography. However, in practice, theoretical performance is rarely achieved due to a combination of undersampling, orthogonality, and refocusing issues prevalent in many LC × LC applications. This is intricately linked to the column dimensions, flow rates, and mobile-phase compositions used, where, in many cases, incompatible or strong solvents are introduced in the second-dimension (D) column, leading to peak broadening and the need for more complex interfacing approaches. In this contribution, the combination of temperature-responsive (TR) and reversed-phase (RP) LC is demonstrated, which, due to the purely aqueous mobile phase used in TRLC, allows for complete and more generic refocusing of organic solutes prior to the second-dimension RP separation using a conventional 10-port valve interface. Thus far, this was only possible when combining other purely aqueous modes such as ion exchange or gel filtration chromatography with RPLC, techniques which are limited to the analysis of charged or high MW solutes, respectively. This novel TRLC × RPLC combination relaxes undersampling constraints and complete refocusing and therefore offers novel possibilities in the field of LC × LC including temperature modulation. The concept is illustrated through the TRLC × RPLC analysis of mixtures of neutral organic solutes.

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Section: Temperature-responsive Chromatographymentioning

confidence: 99%

Enhancing the Possibilities of Comprehensive Two-Dimensional Liquid Chromatography through Hyphenation of Purely Aqueous Temperature-Responsive and Reversed-Phase Liquid Chromatography

Baert¹,

Martens²,

Desmet

et al. 2018

Anal. Chem.

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“…Four steroids were used to evaluate the temperature sensitivity and the separation performance of the silica materials at 10, 30, and 50 °C [56,57]. As shown in Figure 4, the retention time of the steroids and the number of peaks increased with increasing temperature.…”

Section: Resultsmentioning

confidence: 99%

Combination of Three Functionalized Temperature-Sensitive Chromatographic Materials for Serum Protein Analysis

Sun

Dai

et al. 2019

Molecules

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We have developed a methodology to capture acidic proteins, alkaline proteins, and glycoproteins separately in mouse serum using a combination of three functionalized temperature-responsive chromatographic stationary phases. The temperature-responsive polymer poly(N-isopropylacrylamide) was attached to the stationary phase, silica. The three temperature-responsive chromatographic stationary phase materials were prepared by reversible addition–fragmentation chain transfer polymerization. Alkaline, acidic, and boric acid functional groups were introduced to capture acidic proteins, alkaline proteins, and glycoproteins, respectively. The protein enrichment and release properties of the materials were examined using the acidic protein, bovine serum albumin; the alkaline protein, protamine; and the glycoprotein, horseradish peroxidase. Finally, the three materials were used to analyze mouse serum. Without switching the mobile phase, the capture and separation of mouse serum was achieved by the combination of three temperature-responsive chromatographic stationary phase materials. On the whole, 313 proteins were identified successfully. The number of different proteins identified using the new method was 1.46 times greater than the number of proteins that has been identified without applying this method. To our knowledge, this method is the first combinatorial use of three functionalized temperature-responsive chromatographic stationary phase silica materials to separate proteins in mouse serum.

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“…The separation effect of grafted stationary phase was significantly enhanced and retention time was extended with the increase in temperature . The separation of compounds on the thermally responsive column could be performed with an aqueous mobile phase without any organic solvent , which will be beneficial for reducing the environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

“…The separation of compounds on the thermally responsive column could be performed with an aqueous mobile phase without any organic Abbreviations: LCST, lower critical solution temperature; NIPAAm, N-isopropylacrylamide; PMDETA, N,N,N',N'',N'',-pentamethylenetriamine; TGA, thermogravimetric analysis Conflict of Interest: The authors have declared no conflict of interest. solvent [13][14][15][16], which will be beneficial for reducing the environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

Effect of the grafting ratio of poly(N‐isopropylacrylamide) on thermally responsive polymer brush surfaces

Shen

Dong

Liang

et al. 2016

J of Separation Science

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Four kinds of poly(N-isopropylacrylamide)-grafted silicas with different grafting ratios and the same grafting density were prepared by atom transfer radical polymerization. The chemical groups in the stationary phase was verified by FTIR spectroscopy, and the content of elements was measured by elemental analysis. The grafting ratio of thermoresponsive chromatographic materials was measured by thermogravimetric analysis and was 2.36-21.10% mg/m . The retention behaviors of the stationary phase with different grafting ratios were evaluated by separating five kinds of steroids and ten kinds of different hydrophobic properties compounds. With the increase in grafting ratio, the retention time of analytes was prolonged in high-performance liquid chromatography. The results showed that grafting ratio had a significant influence on the separation effect under the same grafting density. And the optimal grafting ratio of poly(N-isopropylacrylamide) was 5.81-13.15%.

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Preparation and characterization of temperature‐responsive chromatographic column containing poly(N‐isopropylacrylamide) and poly([2‐(methacryloyloxy)‐ ethyl]trimetylammonium chloride)

Cited by 16 publications

References 25 publications

Enhancing the Possibilities of Comprehensive Two-Dimensional Liquid Chromatography through Hyphenation of Purely Aqueous Temperature-Responsive and Reversed-Phase Liquid Chromatography

Enhancing the Possibilities of Comprehensive Two-Dimensional Liquid Chromatography through Hyphenation of Purely Aqueous Temperature-Responsive and Reversed-Phase Liquid Chromatography

Combination of Three Functionalized Temperature-Sensitive Chromatographic Materials for Serum Protein Analysis

Effect of the grafting ratio of poly(N‐isopropylacrylamide) on thermally responsive polymer brush surfaces

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