Preparation and characterization of the encapsulated myrtle extract nanoliposome and nanoniosome without using cholesterol and toxic organic solvents: A comparative study

Gorjian, Hayedeh; Amiri, Zeynab Raftani; Milani, Jafar Mohammadzadeh; Khaligh, Nader Ghaffari

doi:10.1016/j.foodchem.2020.128342

Cited by 27 publications

(11 citation statements)

References 25 publications

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“…Based on our research, only one study on myrtle extract in vesicle formulation was reported. Despite this, the extract was obtained from myrtle leaves [ 33 ]. In our study, liposomes were prepared by using a simple and fast method based on the sonication of phospholipids and the myrtle berry extract in the dispersant solution.…”

Section: Discussionmentioning

confidence: 99%

Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application

et al. 2022

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Many substances in plant extracts are known for their biological activities. These substances act in different ways, exerting overall protective effects against many diseases, especially skin disorders. However, plant extracts’ health benefits are often limited by low bioavailability. To overcome these limitations, drug delivery systems can be employed. In this study, we evaluated the antioxidant power of an ethanolic extract from Myrtus communis L. (myrtle) berries through colorimetric tests (DPPH and FRAP). The antioxidant activity was also verified by using fibroblast cell culture through cellular Reactive Oxygen Species (ROS) levels measurements. Moreover, the myrtle extract was formulated in phospholipid vesicles to improve its bioavailability and applicability. Myrtle liposomes were characterized by size, surface charge, storage stability, and entrapment efficiency; visualized by using cryo-TEM images; and assayed for cytocompatibility and anti-ROS activity. Our results suggest that myrtle liposomes were cytocompatible and improved the extract’s antioxidant power in fibroblasts, suggesting a potential skin application for these formulations and confirming that nanotechnologies could be a valid tool to enhance plant extracts’ potentialities.

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Section: Discussionmentioning

confidence: 99%

Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application

et al. 2022

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“…Additionally, the polydispersity index (PdI) of lipid-based nanovesicles may affect their stability and is considered a key factor in particle size distribution. PdI values can range from 0 to 1.0 for an adequately monodisperse and polydisperse nanovesicle, respectively [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

“…Zeta potential measurement is a common practice to determine the surface charge of colloidal systems, repulsion forces between them, and their physical stability [ 28 ]. Hence, the stability of nanoliposomes directly increases the increase in the repulsion of particles by either the electrostatic or steric repulsion of vesicles [ 20 ]. Acceptable zeta potential values to consider a system as stable range from lower than −30 mV to higher +30 mV [ 29 ] and are considered suitable for the colloidal stability of nanoliposomes [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

et al. 2021

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The development of greener nano-constructs with noteworthy biological activity is of supreme interest, as a robust choice to minimize the extensive use of synthetic drugs. Essential oils (EOs) and their constituents offer medicinal potentialities because of their extensive biological activity, including the inhibition of fungi species. However, their application as natural antifungal agents are limited due to their volatility, low stability, and restricted administration routes. Nanotechnology is receiving particular attention to overcome the drawbacks of EOs such as volatility, degradation, and high sensitivity to environmental/external factors. For the aforementioned reasons, nanoencapsulation of bioactive compounds, for instance, EOs, facilitates protection and controlled-release attributes. Nanoliposomes are bilayer vesicles, at nanoscale, composed of phospholipids, and can encapsulate hydrophilic and hydrophobic compounds. Considering the above critiques, herein, we report the in-house fabrication and nano-size characterization of bioactive oregano essential oil (Origanum vulgare L.) (OEO) molecules loaded with small unilamellar vesicles (SUV) nanoliposomes. The study was focused on three main points: (1) multi-compositional fabrication nanoliposomes using a thin film hydration–sonication method; (2) nano-size characterization using various analytical and imaging techniques; and (3) antifungal efficacy of as-developed OEO nanoliposomes against Trichophyton rubrum (T. rubrum) by performing the mycelial growth inhibition test (MGI). The mean size of the nanoliposomes was around 77.46 ± 0.66 nm and 110.4 ± 0.98 nm, polydispersity index (PdI) of 0.413 ± 0.015, zeta potential values up to −36.94 ± 0.36 mV were obtained by dynamic light scattering (DLS). and spherical morphology was confirmed by scanning electron microscopy (SEM). The presence of OEO into nanoliposomes was displayed by attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. Entrapment efficiency values of 79.55 ± 6.9% were achieved for OEO nanoliposomes. In vitro antifungal activity of nanoliposomes tested against T. rubrum strains revealed that OEO nanoliposomes exhibited the highest MGI, 81.66 ± 0.86%, at a concentration of 1.5 µL/mL compared to the rest of the formulations. In summary, this work showed that bioactive OEO molecules with loaded nanoliposomes could be used as natural antifungal agents for therapeutical purposes against T. rubrum.

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“…The particle size of prepared liposomes was determined by a particle size analyzer (Zetasizer NanoZS 90, Malvern Company). The encapsulation efficiency (%) of C3G liposomes was measured by previous study to quantify the concentration of encapsulated C3G in the aqueous phase (Gorjian et al., 2021 ). A 2‐ml aliquot of the prepared liposome solution was collected and centrifuged by using a freeze centrifuge (Beckman) at 7104 g for 30 min at 4°C, and the total amount of C3G absorbance of each sample was recorded at 540 nm by a UV spectrophotometry(UV‐1200, China).…”

Section: Methodsmentioning

confidence: 99%

Anti‐inflammatory activity of cyanidin‐3‐O‐glucoside and cyanidin‐3‐O‐glucoside liposomes in THP‐1 macrophages

Hao

Guan

Huang

et al. 2021

Food Science & Nutrition

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Inflammation is a response of the body to stimulation, which may be beneficial or harmful. Inflammation may either be acute or chronic, and is related to the homeostasis of physiological systems in vivo. Inflammation is associated with many diseases and is the basis of most pathological processes. The main causes of inflammation are infection and tissue damage, which can lead to the concentration of leukocytes and plasma proteins (Medzhitov, 2008).Chronic inflammation is involved in many diseases, including diabetes, obesity, atherosclerosis, and cancer (Coussens & Werb, 2002;

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Preparation and characterization of the encapsulated myrtle extract nanoliposome and nanoniosome without using cholesterol and toxic organic solvents: A comparative study

Cited by 27 publications

References 25 publications

Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application

Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application

Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

Anti‐inflammatory activity of cyanidin‐3‐O‐glucoside and cyanidin‐3‐O‐glucoside liposomes in THP‐1 macrophages

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