Preparation and cytotoxicity of N,N,N-trimethyl chitosan/alginate beads containing gold nanoparticles

Abstract

Polyelectrolyte complex beads based on N,N,N-trimethyl chitosan (TMC) and sodium alginate (ALG) were obtained. This biomaterial was characterised by FTIR, TGA/DTG, DSC and SEM analysis. The good properties of polyelectrolyte complex hydrogel beads were associated, for the first time, with gold nanoparticles (AuNPs). Through a straightforward methodology, AuNPs were encapsulated into the beads. The in vitro cytotoxicity assays on the Caco-2 colon cancer cells and healthy VERO cells showed that the beads presented good biocompatibility on both cell lines, whereas the beads loaded with gold nanoparticles (beads/AuNPs) was slightly cytotoxic on the Caco-2 and VERO cells.

Polyelectrolyte complex containing silver nanoparticles with antitumor property on Caco-2 colon cancer cells

“…Sodium alginate (ALG) was purchased from Across Organics (NJ, USA) and the ratio of mannuronic acid to guluronic acid (M/G) of the ALG was 1.56, as stated by the manufacturer. It has already been reported that the values of the average number (Mn) and average-weight (Mw) molecular weights for this alginate are 339,000 g mol −1 and 1,073,000 g mol −1 , respectively [22]. Silver nitrate, sodium citrate, potassium dihydrogen phosphate and acetic acid were purchased from Sigma-Aldrich.…”

“…The methodology employed for the preparation of beads was based on the method published by Martins et al [21,22,24]. Scheme 1 depicts the preparation of beads based on TMC/ALG.…”

Liposome as nanocarrier: Site targeted delivery in lung cancer

“…For the cancerous cells, AuNPs are being utilized as salutary and incongruous agent for cancer disclosure. NPs have charged surface that can fasten arbitrarily to biological molecules through the electrostatic interactions [25]. For the configuration of electrochemical and other types of biosensors, nanoparticle-based materials are used as they give the great sensitivity and specificity.…”

N,N-Dimethyl chitosan/heparin polyelectrolyte complex vehicle for efficient heparin delivery

“…The resonance signal at ı 2.05 ppm presented in the CHT 1 H NMR spectrum (Fig. 1a) was assigned to the hydrogen atoms of acetylated residues; the resonance at ı 3.16 ppm was assigned to the hydrogen atoms (H2); the resonances observed between ı 3.61 and ı 4.45 ppm were assigned to the hydrogen atoms (H3-H6) and resonances of low intensity that occur in the spectral range between ı 4.5 and ı 5.5 ppm were assigned to the hydrogen atoms (H1) [28][29][30]. However, the intense resonance in the spectral range ı 4.72 to ı 5.00 was attributed to hydrogen resonance of water molecules.…”

“…DMC was obtained with dimethylation degree (DD) of ca. 86% [30,31]. The DD was obtained using Eq (1), from the area attributed to resonance of hydrogen atoms on N-dimethylated sites N(CH 3 ) 2 , at ı 3.10 ppm, and from area of H2 resonance at ı 3.43 ppm (Fig.…”