Preparation and degradation of l-lactide and ε-caprolactone homo and copolymer films

Molinuevo

2008

J Tissue Eng Regen Med

Biodegradable and biocompatible polymeric scaffolds have been recently introduced for tissue regeneration purpose. In the present study we aimed to develop polymeric-based scaffolds for bone regeneration. Two polyesters, poly-β-propiolactone (PBPL), poly-ε-caprolactone (PCPL) and two polyfumarates, polydiisopropyl fumarate (PDIPF), polydicyclohexyl fumarate (PDCF) were chosen to prepare films which can support osteoblastic growth. Scanning electron microscopy and water contact angle were used to characterize the matrices. Biodegradation studies were performed both in PBS buffer and using an in vitro macrophage degradation assay. Mouse calvaria-derived MC3T3E1 cells and UMR106 rat osteosarcoma cell lines were used to perform biocompatibility and cytotoxicity studies. The polyesters, the most hydrophilic polymers studied, showed a rougher and more porous surfaces than the polyfumarates. Under acellular conditions, only PBPL was degraded by hydrolytic mechanisms. However, macrophages performed an active degradation of all polymeric films. Osteoblasts developed well-defined actin fibres without evidence of cytotoxicity when growing on the films. The number of UMR106 osteoblasts that adhered to the PBPL-based film was higher than that of the cells attached to the PECL and polyfumarates (PDIPF and PDCF) matrices. Both UMR106 and MC3T3E1 osteoblastic lines showed protein levels comparable to control conditions, demonstrating that they grew well on all surfaces. However, UMR106 cells showed a significant increase in proliferation on polyester-derived scaffolds (PBPL and PECL). The alkaline phosphatase activity of UMR106, an osteoblastic marker, was significantly higher than that of control plastic dishes. MC3T3E1 cells expressed similar levels of this differentiation marker in all polymeric matrices. We found similar collagen protein content after 48 h culture of UMR106 cells on all surfaces. However, important differences were evident in the MC3T3E1 line. In conclusion, the synthetic polymeric-based scaffold we have developed and studied supports adhesion, growth and differentiation of two osteoblastic cell lines, suggesting that they could be useful in bone tissue regeneration.

Section: Discussionmentioning

confidence: 99%

Biocompatibility and biodegradation of polyester and polyfumarate based-scaffolds for bone tissue engineering

Molinuevo

2008

J Tissue Eng Regen Med

Braz. arch. biol. technol.

“…The annual disposal of plastics has raised the demand for nondegradable plastic materials. Selective use of biodegradable polymers in certain applications might help to reduce the environmental impact of plastic materials (Yavuz et al, 2002). These materials became a waste concern, because of their persistence in the environment (Aboud-Zeid et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Biotreatment effects in films and blends of PVC/PCL previously treated with heat

Campos

Franchetti

2005

“…For example, Huang et al 51 studied the degradation of a PLA-PCL-PLA triblock polymer in phosphate buffer solution (PBS). Other authors have used different enzyme solutions such as lipase 52 or proteinase, 53 as well as in the presence of microorganisms 54 to determine the degradation kinetics of materials. We used DMEM + FBS because we believe this medium is more representative of physiological conditions than with lipase or PBS.…”

Section: Fernández Et Almentioning

confidence: 99%

Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility

Fernández¹,

Cortizo²,

Cortizo³

2014

j biomater tissue eng

The present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly--caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco's modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL + HAP or Blend + HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer-and HAP-based scaffolds could potentially be used as bone substitute materials.