Preparation and evaluation of 2-methylimidazolium-functionalized silica as a mixed-mode stationary phase for hydrophilic interaction and anion-exchange chromatography

Yang, Bo; Liu, Houmei; Chen, Jia; Guan, Ming; Qiu, Hongdeng

doi:10.1016/j.chroma.2016.09.021

Cited by 45 publications

(15 citation statements)

References 35 publications

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“…S4), and the corresponding theoretical plates were ∼25 513, 31 013, and 56 355 N/m on the silica‐poly(St‐ b ‐Nass), self‐packed ODS, and commercial ODS columns, respectively. Obviously, the theoretical plate number of silica‐poly(St‐ b ‐Nass) column was lower than the commercial ODS column, but it was close to that of the reported mixed‐mode columns that employed the same silica gel as our prepared diblock copolymer column .…”

Section: Resultssupporting

confidence: 80%

“…This feature suggested the potential application of our proposed method in the preparation of mixed‐mode stationary phase for the analysis of complex samples. Finally, it should be pointed out that the peaks of the test solutes showed a bit broad and tailing on our prepared column, which is frequently found on the silica‐based mixed‐mode stationary phases , and was ascribed to the packing reason of polymer materials and the incomplete suppression of the interaction between negatively charged residual silanol groups and basic analytes.…”

Section: Resultsmentioning

confidence: 73%

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Preparation and evaluation of diblock copolymer‐grafted silica by sequential surface initiated‐atom transfer radical polymerization for reverse‐phase/ion‐exchange mixed‐mode chromatography

Wang

Wei

2017

J of Separation Science

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A novel approach that involved the grafting of diblock copolymer with two types of monomer onto substrate by sequential surface initiated-atom transfer radical polymerization was proposed to prepare a mixed-mode chromatographic stationary phase. The distinguishing feature of this method is that it can be applied in the preparation of various mixed-mode stationary phases. In this study, a new reverse-phase/ion-exchange stationary phase was prepared by grafting hydrophobic styrene and cationic sodium 4-styrenesulfonate by the proposed approach onto silica surface. The chromatographic properties of the prepared stationary phase were evaluated by the separation of benzene derivatives, anilines, and β-agonists, and by the effect of pH values and acetonitrile content on the retention. Compared with typical RP columns, the prepared stationary phase achieved the better resolution and higher selectivity at a shorter separation time and lower organic content. Moreover, the application of the prepared column was proved by separating widely distributed polar and charged compounds simultaneously.

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 73%

Preparation and evaluation of diblock copolymer‐grafted silica by sequential surface initiated‐atom transfer radical polymerization for reverse‐phase/ion‐exchange mixed‐mode chromatography

Wang

Wei

2017

J of Separation Science

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“…2‐Methylimidazoilum‐modified silica microparticles as a new ILs type stationary phase intended for application in HILIC/anion‐exchange regime has been proposed, synthesized, and characterized . Porous spherical silica was suspended in toluene and 2‐methylimidazole and 3‐chloropropyltrimethoxysilane were added.…”

Section: Silica‐based Mixed‐mode Stationary Phasesmentioning

confidence: 99%

Recent advances in mixed‐mode chromatographic stationary phases

Sýkora

Řezanka

Záruba

et al. 2018

J of Separation Science

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Mixed‐mode phases have become very popular in the last decade, and the number of new mixed/multi‐mode sorbents is growing fast. Unlike single‐mode stationary phases, perfectly suited for the separation of the analytes possessing similar physicochemical properties, for instance reversed‐phase chromatography for hydrophobic solutes, mixed‐mode sorbents providing multimodal interactions can render better separation selectivity for complex mixtures of solutes differing significantly in their physicochemical characteristics. The most frequent modern mixed‐mode stationary phases are di/tri‐mode sorbents embracing the following interactions, hydrophobic, electrostatic (coulombic), and hydrophilic. According to their structures, it is possible to distinguish silica‐based, polymer‐based, hybrid, and monolithic mixed‐mode stationary phases. Herewith, newly synthesized mixed‐mode sorbents developed within the last two and half years are categorized, discussed, and summarized. The main attention is devoted to the description of the synthetic routes and characterization methods applied for the new stationary phases.

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“…They evaluated its chromatographic performance with RPLC, HILIC and anion exchange modes. In Qiu and co-workers' report, 21 2-methylimidazolium-functionalized silica stationary phase was prepared and further used for hydrophilic interaction and anion-exchange mixed-mode chromatography.…”

Section: Introductionmentioning

confidence: 99%

Study on the Mixed-Mode HPLC Separation of Shuang-Huang-Lian and Xue‑Bi‑Jing Injections Based on an Ionic Liquid Column

Yang¹,

Zhang²,

Zhang³

et al. 2018

J. Braz. Chem. Soc.

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Both Shuang-Huang-Lian and Xue-Bi-Jing injections are clinically proven efficient and low cost medicines. However, the studies of their chromatographic separation are limited. In this study, a column based on ionic liquid stationary phase was applied for the first time for the analysis of these two injections. For Shuang-Huang-Lian injection, U-shaped plots of retention factors vs. mobile phase constitutions were obtained. This unique kind of separation behavior allowed a flexible adjustment of retention by tuning the mobile phase constituents. Then acids group was analyzed with hydrophilic chromatography mode, while glycosides group was analyzed with reversed phase chromatography mode. Similarly, constituents in Xue-Bi-Jing injection were analyzed with mixed mode high performance liquid chromatography (HPLC). Higher, medium and lower polarity groups were analyzed with hydrophilic interaction, reversed phase and normal phase liquid chromatography modes, respectively. Results from this work provided valuable information for the separation of complicated systems such as traditional Chinese medicine injections.Keywords: ionic liquid stationary phase, mixed-mode chromatography, Shuang-Huang-Lian injection, Xue-Bi-Jing injection IntroductionThe formulations of traditional Chinese medicines (TCMs) are becoming increasingly popular worldwide, mainly because of their high efficiency and low cost. However, TCM formulations are usually based on a whole plant and/or combination of several herbs, which contain more than dozens to hundreds of components. 1 Therefore, in order to ensure both the safety and efficacy of TCM formulations, it is necessary to establish simple and effective methods to determine as many constituents as possible.2,3 Liquid chromatography (LC) has been applied as a rapid and economic method for the determination of multi-component in TCMs. 4 Among the liquid chromatography analytical methods, high performance liquid chromatography (HPLC) is the most popular one, due to its easy operation, wide suitability and high accuracy for the qualitative and quantitative analysis of TCMs. [5][6][7] However, in practice, some factors, such as efficiency, selectivity and sensitivity have been found to affect the analysis of complicated systems with HPLC. 8Chromatographic stationary phase is the core of HPLC system. Therefore, the development of novel stationary phases with high separation efficiency and excellent selectivity has always been the focus point of improving the separating ability of HPLC. Accordingly, in this work novel ionic liquid (IL) bonded stationary phase has been studied. IL is a kind of novel material composed of ions and at the same time remains in liquid state at a temperature below 100 °C.9 ILs have demonstrated the versatility and tuneability in separation science. 10 Many types of ILs were immobilized on a support like silica or monolith as stationary phases for liquid chromatography. 11,12 Due to the designable character of the IL molecule, multi interactions between the IL stationary phas...

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Preparation and evaluation of 2-methylimidazolium-functionalized silica as a mixed-mode stationary phase for hydrophilic interaction and anion-exchange chromatography

Cited by 45 publications

References 35 publications

Preparation and evaluation of diblock copolymer‐grafted silica by sequential surface initiated‐atom transfer radical polymerization for reverse‐phase/ion‐exchange mixed‐mode chromatography

Preparation and evaluation of diblock copolymer‐grafted silica by sequential surface initiated‐atom transfer radical polymerization for reverse‐phase/ion‐exchange mixed‐mode chromatography

Recent advances in mixed‐mode chromatographic stationary phases

Study on the Mixed-Mode HPLC Separation of Shuang-Huang-Lian and Xue‑Bi‑Jing Injections Based on an Ionic Liquid Column

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