Preparation and evaluation of a polymer-based sulfobetaine zwitterionic stationary phase

Simultaneous chromatographic separation of the anomers of saccharides on a polymer sulfobetaine zwitterionic stationary phase

“…The chromatographic analysis was performed on a LC‐20AD system (Shimadzu) coupled with an evaporative light scattering detector (Wayeal Corp.). A self‐made PSZ column was used here, as described previously [22]. In addition, two zwitterionic columns were also tested, including a silica‐based sulfobetaine functionalized zwitterionic column (SSZ), prepared by the same grafting method as PSZ, and a polymer‐based ester‐type sulfobetaine functionalized zwitterionic column (PESZ), prepared by the same substrate as PSZ except that ester‐type sulfobetaine monomer was used to replace amide‐type one used in PSZ.…”

“…The chromatographic analysis was performed on a LC-20AD system (Shimadzu) coupled with an evaporative light scattering detector (Wayeal Corp.). A self-made PSZ column was used here, as described previously [22].…”

“…Hetrick et al also reported the separation of dextrose anomers via a commercial zwitterionic columns [16], while only one pair dextrose anomers were provided. More recently, we have developed a polymerbased sulfobetaine functionalized zwitterionic stationary phase (PSZ), which showed good hydrophilic property and good chromatographic performance [22]. Initial tests showed that it exhibited excellent separation performance toward the anomer of saccharides.…”

Application of chromatography in purification and structural analysis of natural polysaccharides: A review

“…In 1975, the first generation of HILIC mode on the aminosilica phase was used for the separation of saccharides [62]. With the development of HILIC, different types of stationary phases with various ligands emerged in saccharide separation studies, including diol bonded [63][64][65][66], amino-bonded [67,68], amide-bonded [69][70][71][72], and polymer-bonded phases [73,74], etc. Several published experimental cases of the separation of saccharides on different types of stationary phases are listed in Table 1.…”

Rapid fabrication of zwitterionic sulfobetaine vinylimidazole-based monoliths via photoinitiated copolymerization for hydrophilic interaction chromatography

“…For instance, poly(sulfobetaine), employed as an implant material and device, can reduce protein adsorption and limit cellular adhesion and inflammatory response [ 3 ]. Various sulfobetaine-based packing columns, including commercial zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) [ 5 ], N , N -dimethyl- N -methacryloxyethyl- N -(3-sulfopropyl)ammonium betaine (SPE)- [ 6 ], 3-[2-(acrylamido)-ethyldimethylammonio] propane sulfonate- [ 7 ], and acrylamide-type sulfobetaine-based [ 8 ] stationary phases, have been developed for the separation of polar analytes or enrichment of glycopeptides via hydrophilic or electrostatic interactions [ 9 ]. Although good chromatographic performance was obtained, the preparation process of the packed column was very complicated, which limits its further application [ [10] , [11] , [12] ].…”